Abstract
Different tests for the identification of Gardnerella (Haemophilus) vaginalis and for its differentiation from catalase-negative unclassified coryneforms from the vagina were evaluated on over 200 bacterial strains, with special emphasis on optimal test conditions. A presumptive identification of G. vaginalis in the clinical laboratory can be made on the basis of colonial morphology, clear beta-hemolysis with diffuse edges on human blood bilayer-Tween agar, a negative catalase test, and typical cell morphology in the Gram stain. This procedure will correctly identify 90 to 98% of suspect colonies of G. vaginalis with human blood bilayer-Tween agar as primary isolation medium. Useful additional reactions for the confirmation of G. vaginalis include positive hippurate and starch hydrolysis, positive alpha-glucosidase but negative beta-glucosidase tests, the production of acid from glucose and maltose but not from mannitol, and susceptibility to disks containing metronidazole, nitrofurantoin, sulfonamides, and bile.
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Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bailey R. K., Voss J. L., Smith R. F. Factors affecting isolation and identification of Haemophilus vaginalis (Corynebacterium vaginale). J Clin Microbiol. 1979 Jan;9(1):65–71. doi: 10.1128/jcm.9.1.65-71.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dunkelberg W. E., Jr, McVeigh I. Growth requirements of Haemophilus vaginalis. Antonie Van Leeuwenhoek. 1969;35(2):129–145. doi: 10.1007/BF02219124. [DOI] [PubMed] [Google Scholar]
- Dunkelberg W. E., Jr, Skaggs R., Kellogg D. S., Jr A study and new description of Corynebacterium vaginale (Haemophilus vaginalis). Am J Clin Pathol. 1970 Mar;53(3):370–377. doi: 10.1093/ajcp/53.3.370. [DOI] [PubMed] [Google Scholar]
- Dunkelberg W. E., Jr, Skaggs R., Kellogg D. S., Jr Method for isolation and identification of Corynebacterium vaginale (Haemophilus vaginalis). Appl Microbiol. 1970 Jan;19(1):47–52. doi: 10.1128/am.19.1.47-52.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Greenwood J. R., Pickett M. J. Salient features of Haemophilus vaginalis. J Clin Microbiol. 1979 Feb;9(2):200–204. doi: 10.1128/jcm.9.2.200-204.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Harvey S. M. Hippurate hydrolysis by Campylobacter fetus. J Clin Microbiol. 1980 Apr;11(4):435–437. doi: 10.1128/jcm.11.4.435-437.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Malone B. H., Schreiber M., Schneider N. J., Holdeman L. V. Obligately anaerobic strains of Corynebacterium vaginale (Haemophilus vaginalis). J Clin Microbiol. 1975 Sep;2(3):272–275. doi: 10.1128/jcm.2.3.272-275.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Piot P., van Dyck E., Goodfellow M., Falkow S. A taxonomic study of Gardnerella vaginalis (Haemophilus vaginalis) Gardner and Dukes 1955. J Gen Microbiol. 1980 Aug;119(2):373–396. doi: 10.1099/00221287-119-2-373. [DOI] [PubMed] [Google Scholar]
- Smith R. F. New medium for isolation of Corynebacterium vaginale from genital specimens. Health Lab Sci. 1975 Jul;12(3):219–224. [PubMed] [Google Scholar]
- Smith R. F., Voss J. L., Bailey R. K. Tellurite reduction test to aid in the recognition of Corynebacterium vaginale. J Clin Microbiol. 1977 Mar;5(3):375–377. doi: 10.1128/jcm.5.3.375-377.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Totten P. A., Amsel R., Hale J., Piot P., Holmes K. K. Selective differential human blood bilayer media for isolation of Gardnerella (Haemophilus) vaginalis. J Clin Microbiol. 1982 Jan;15(1):141–147. doi: 10.1128/jcm.15.1.141-147.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Vecchio T. J. Predictive value of a single diagnostic test in unselected populations. N Engl J Med. 1966 May 26;274(21):1171–1173. doi: 10.1056/NEJM196605262742104. [DOI] [PubMed] [Google Scholar]