Abstract
The AutoMicrobic system (AMS) Enterobacteriaceae-plus Biochemical Card was developed to identify a select group of 10 species of glucose-nonfermentative and oxidase-positive fermentative gram-negative bacilli. In this study, 159 nonenteric clinical isolates were identified by the AMS and conventional tube biochemicals based on E. O. King's (Centers for Disease Control) identification schema. The AMS properly identified 96.7% (117 of 121) of isolates whose taxa were included in the AMS data base. Of 38 isolates (94.7%) in which taxa were not included in the data base, 36 were correctly called unidentified organisms. A principal advantage of the AMS is the automated identification of frequently isolated nonenterics in a period of only 8 to 13 h. The AMS, with the use of the Enterobacteriaceae-plus Biochemical Card appears to be a rapid and accurate system for the identification of the most commonly isolated nonfermentative and oxidase-positive fermentative gram-negative bacilli.
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Selected References
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- Aldridge C., Jones P. W., Gibson S., Lanham J., Meyer M., Vannest R., Charles R. Automated microbiological detection/identification system. J Clin Microbiol. 1977 Oct;6(4):406–413. doi: 10.1128/jcm.6.4.406-413.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Burdash N. M., Bannister E. R., Manos J. P., West M. E. A comparison of four commercial systems for the identification of nonfermentative gram-negative bacilli. Am J Clin Pathol. 1980 Apr;73(4):564–569. doi: 10.1093/ajcp/73.4.564. [DOI] [PubMed] [Google Scholar]
- Ferraro M. J., Edelblut M. A., Kunz L. J. Accurate automated identification of selected Enterobacteriaceae at four hours. J Clin Microbiol. 1981 Jan;13(1):151–157. doi: 10.1128/jcm.13.1.151-157.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hasyn J. J., Cundy K. R., Dietz C. C., Wong W. Clinical laboratory evaluation of the Auto-Microbic system for rapid identification of Enterobacteriaceae. J Clin Microbiol. 1981 Mar;13(3):491–497. doi: 10.1128/jcm.13.3.491-497.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Isenberg H. D., Gavan T. L., Smith P. B., Sonnenwirth A., Taylor W., Martin W. J., Rhoden D., Balows A. Collaborative investigation of the AutoMicrobic System Enterobacteriaceae biochemical card. J Clin Microbiol. 1980 Jun;11(6):694–702. doi: 10.1128/jcm.11.6.694-702.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Warwood N. M., Blazevic D. J., Hofherr L. Comparison of the API 20E and Corning N/F systems for identification of nonfermentative gram-negative rods. J Clin Microbiol. 1979 Aug;10(2):175–179. doi: 10.1128/jcm.10.2.175-179.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]