Skip to main content
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1982 Jan;15(1):181–183. doi: 10.1128/jcm.15.1.181-183.1982

Production of K88, K99, and 987P antigens by Escherichia coli cultured on synthetic and complex media.

D H Francis, G A Remmers, P S DeZeeuw
PMCID: PMC272050  PMID: 6764769

Abstract

A synthetic medium (E agar) containing glucose and citric acid as the only organic nutrients was found superior to blood agar, Minca-IsoVitaleX (BBL Microbiology Systems, Cockeysville, Md.) agar and broth, and tryptic soy broth (without glucose) for promoting expression of K99 antigen by Escherichia coli strains isolated from diarrheic calves and piglets.

Full text

PDF
181

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Guinée P. A., Veldkamp J., Jansen W. H. Improved minca medium for the detection of K99 antigen in calf enterotoxigenic strains of Escherichia coli. Infect Immun. 1977 Feb;15(2):676–678. doi: 10.1128/iai.15.2.676-678.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Isaacson R. E., Moon H. W., Schneider R. A. Distribution and virulence of Escherichia coli in the small intestines of calves with and without diarrhea. Am J Vet Res. 1978 Nov;39(11):1750–1755. [PubMed] [Google Scholar]
  3. Middeldorp J. M., Witholt B. K88-mediated binding of Escherichia coli outer membrane fragments to porcine intestinal epithelial cell brush borders. Infect Immun. 1981 Jan;31(1):42–51. doi: 10.1128/iai.31.1.42-51.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Moon H. W., Kohler E. M., Schneider R. A., Whipp S. C. Prevalence of pilus antigens, enterotoxin types, and enteropathogenicity among K88-negative enterotoxigenic Escherichia coli from neonatal pigs. Infect Immun. 1980 Jan;27(1):222–230. doi: 10.1128/iai.27.1.222-230.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Stirm S., Orskov F., Orskov I., Mansa B. Episome-carried surface antigen K88 of Escherichia coli. II. Isolation and chemical analysis. J Bacteriol. 1967 Feb;93(2):731–739. doi: 10.1128/jb.93.2.731-739.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. VOGEL H. J., BONNER D. M. Acetylornithinase of Escherichia coli: partial purification and some properties. J Biol Chem. 1956 Jan;218(1):97–106. [PubMed] [Google Scholar]
  7. de Graaf F. K., Wientjes F. B., Klaasen-Boor P. Production of K99 antigen by enterotoxigenic Escherichia coli strains of antigen groups o8, o9, o20, and o101 grown at different conditions. Infect Immun. 1980 Jan;27(1):216–221. doi: 10.1128/iai.27.1.216-221.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES