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. 1982 Feb;15(2):216–219. doi: 10.1128/jcm.15.2.216-219.1982

Differentiation of Haemophilus spp. in Respiratory isolate cultures by an indole spot test.

D F Welch, P A Ahlin, J M Matsen
PMCID: PMC272063  PMID: 7040445

Abstract

Indole spot tests using isolated, nonhemolytic colonies of Haemophilus species were positive for 90 of 151 (60%) respiratory isolates of Haemophilus influenzae, whereas 67 to 72 (93%) isolates of H. influenzae from cerebrospinal fluid and blood specimens were indole positive. Only 4 of 117 (3%) Haemophilus parainfluenzae isolates were positive for indole spot tests. Thus, indole-positive, nonhemolytic Haemophilus isolates in respiratory cultures can be presumptively identified as H. influenzae.

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Selected References

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  1. Albritton W. L., Penner S., Slaney L., Brunton J. Biochemical characteristics of Haemophilus influenzae in relationship to source of isolation and antibiotic resistance. J Clin Microbiol. 1978 Jun;7(6):519–523. doi: 10.1128/jcm.7.6.519-523.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Edberg S. C., Melton E., Singer J. M. Rapid biochemical characterization of Haemophilus species by using the micro-ID. J Clin Microbiol. 1980 Jan;11(1):22–26. doi: 10.1128/jcm.11.1.22-26.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Eng R. H., Corrado M. L., Cleri D., Sierra M. F. Non-type b Haemophilus influenzae infections in adults with reference to biotype. J Clin Microbiol. 1980 Jun;11(6):669–671. doi: 10.1128/jcm.11.6.669-671.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Gandelman A. L. The use of reagent impregnated paper strips as an aid in the identification of certain gram negative organisms. Am J Med Technol. 1966 Mar-Apr;32(2):85–87. [PubMed] [Google Scholar]
  5. Golberg R., Washington J. A., 2nd The taxonomy and antimicrobial susceptibility of Haemophilus species in clinical specimens. Am J Clin Pathol. 1978 Dec;70(6):899–904. doi: 10.1093/ajcp/70.6.899. [DOI] [PubMed] [Google Scholar]
  6. Hicks M. J., Ryan K. J. Simplified scheme for identification of prompt lactose-fermenting members of the Enterobacteriaceae. J Clin Microbiol. 1976 Dec;4(6):511–514. doi: 10.1128/jcm.4.6.511-514.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Kilian M. A rapid method for the differentiation of Haemophilus strains. The porphyrin test;. Acta Pathol Microbiol Scand B Microbiol Immunol. 1974 Dec;82(6):835–842. doi: 10.1111/j.1699-0463.1974.tb02381.x. [DOI] [PubMed] [Google Scholar]
  8. Kilian M. A taxonomic study of the genus Haemophilus, with the proposal of a new species. J Gen Microbiol. 1976 Mar;93(1):9–62. doi: 10.1099/00221287-93-1-9. [DOI] [PubMed] [Google Scholar]
  9. Kilian M., Sørensen I., Frederiksen W. Biochemical characteristics of 130 recent isolates from Haemophilus influenzae meningitis. J Clin Microbiol. 1979 Mar;9(3):409–412. doi: 10.1128/jcm.9.3.409-412.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Klein M., Blazevic D. J. Evaluation of a selective medium for isolation of Haemophilus from respiratory cultures. Am J Med Technol. 1970 Mar;36(3):97–106. [PubMed] [Google Scholar]
  11. Lowrance B. L., Reich P., Traub W. H. Evaluation of two spot-indole reagents. Appl Microbiol. 1969 Jun;17(6):923–924. doi: 10.1128/am.17.6.923-924.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Lund M. E., Blazevic D. J. Rapid speciation of Haemophilus with the porphyrin production test versus the satellite test for X. J Clin Microbiol. 1977 Feb;5(2):142–144. doi: 10.1128/jcm.5.2.142-144.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Oberhofer T. R., Back A. E. Biotypes of Haemophilus encountered in clinical laboratories. J Clin Microbiol. 1979 Aug;10(2):168–174. doi: 10.1128/jcm.10.2.168-174.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Shurin P. A., Pelton S. I., Scheifele D., Klein J. O. Otitis media caused by non-typable, ampicillin-resistant strains of Haemophilus influenzae. J Pediatr. 1976 Apr;88(4 Pt 1):646–649. doi: 10.1016/s0022-3476(76)80028-5. [DOI] [PubMed] [Google Scholar]
  15. Sutter V. L., Carter W. T. Evaluation of media and reagents for indole-spot tests in anaerobic bacteriology. Am J Clin Pathol. 1972 Sep;58(3):335–338. doi: 10.1093/ajcp/58.3.335. [DOI] [PubMed] [Google Scholar]
  16. Sutter V. L., Finegold S. M. Haemophilus aphrophilus infections: clinical and bacteriologic studies. Ann N Y Acad Sci. 1970 Oct 30;174(2):468–487. doi: 10.1111/j.1749-6632.1970.tb45574.x. [DOI] [PubMed] [Google Scholar]
  17. Welch D. F., Kelly M. T. Sputum screening by Nomarski interference contrast microscopy. J Clin Microbiol. 1979 Apr;9(4):520–524. doi: 10.1128/jcm.9.4.520-524.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]

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