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. 1982 Feb;15(2):302–307. doi: 10.1128/jcm.15.2.302-307.1982

Evaluation of the AutoMicrobic system for identification of glucose-nonfermenting gram-negative rods.

S M Smith, K R Cundy, G L Gilardi, W Wong
PMCID: PMC272081  PMID: 7040451

Abstract

With the Enterobacteriaceae Plus Biochemical Card, the AutoMicrobic system (Vitek Systems, Inc., Hazelwood, Mo.) is purported to identify members of the family Enterobacteriaceae and seven species of glucose-nonfermenting gram-negative rods: Pseudomonas aeruginosa, P. fluorescens, P. putida, P. maltophilia, P. cepacia, and Acinetobacter calcoaceticus (saccharolytic and non-saccharolytic). The latter capability was examined in this investigation. Of 410 glucose-nonfermenting rods included in the identification profile, the AutoMicrobic system correctly identified 366 (89.3%). Of 62 glucose-nonfermenting organisms not included in the identification profile, 41 (66%) were correctly reported as "unidentified organism." The usefulness of the AutoMicrobic system-generated identification probability in establishing criteria for acceptance of identification is discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aldridge C., Jones P. W., Gibson S., Lanham J., Meyer M., Vannest R., Charles R. Automated microbiological detection/identification system. J Clin Microbiol. 1977 Oct;6(4):406–413. doi: 10.1128/jcm.6.4.406-413.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Appelbaum P. C., Stavitz J., Bentz M. S., von Kuster L. C. Four methods for identification of gram-negative nonfermenting rods: organisms more commonly encountered in clinical specimens. J Clin Microbiol. 1980 Aug;12(2):271–278. doi: 10.1128/jcm.12.2.271-278.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Burdash N. M., Bannister E. R., Manos J. P., West M. E. A comparison of four commercial systems for the identification of nonfermentative gram-negative bacilli. Am J Clin Pathol. 1980 Apr;73(4):564–569. doi: 10.1093/ajcp/73.4.564. [DOI] [PubMed] [Google Scholar]
  4. Chester V., Cleary T. J. Evaluation of the Minitek system for identification of nonfermentative and nonenteric fermentative Gram-negative bacteria. J Clin Microbiol. 1980 Oct;12(4):509–516. doi: 10.1128/jcm.12.4.509-516.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Dowda H. Evaluation of two rapid methods for identification of commonly encountered nonfermenting or oxidase-positive, Gram-negative rods. J Clin Microbiol. 1977 Dec;6(6):605–609. doi: 10.1128/jcm.6.6.605-609.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hasyn J. J., Cundy K. R., Dietz C. C., Wong W. Clinical laboratory evaluation of the Auto-Microbic system for rapid identification of Enterobacteriaceae. J Clin Microbiol. 1981 Mar;13(3):491–497. doi: 10.1128/jcm.13.3.491-497.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Isenberg H. D., Gavan T. L., Smith P. B., Sonnenwirth A., Taylor W., Martin W. J., Rhoden D., Balows A. Collaborative investigation of the AutoMicrobic System Enterobacteriaceae biochemical card. J Clin Microbiol. 1980 Jun;11(6):694–702. doi: 10.1128/jcm.11.6.694-702.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Isenberg H. D., Gavan T. L., Sonnenwirth A., Taylor W. I., Washington J. A., 2nd Clinical laboratory evaluation of automated microbial detection/identification system in analysis of clinical urine specimens. J Clin Microbiol. 1979 Aug;10(2):226–230. doi: 10.1128/jcm.10.2.226-230.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kelly M. T., Latimer J. M. Comparison of the automicrobic system with API, enterotube, micro-ID, micro-media systems, and conventional methods for identification of Enterobacteriaceae. J Clin Microbiol. 1980 Nov;12(5):659–662. doi: 10.1128/jcm.12.5.659-662.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Nicholson D. P., Koepke J. A. The Automicrobic System for urines. J Clin Microbiol. 1979 Dec;10(6):823–833. doi: 10.1128/jcm.10.6.823-833.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Otto L. A., Blachman U. Nonfermentative bacilli: evaluation of three systems for identification. J Clin Microbiol. 1979 Aug;10(2):147–154. doi: 10.1128/jcm.10.2.147-154.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Shayegani M., Lee A. M., McLynn D. M. Evaluation of the Oxi/Ferm tube system for identification of nonfermentative Gram-negative bacilli. J Clin Microbiol. 1978 Jun;7(6):533–538. doi: 10.1128/jcm.7.6.533-538.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Shayegani M., Maupin P. S., McGlynn D. M. Evaluation of the API 20E system for identification of nonfermentative Gram-negative bacteria. J Clin Microbiol. 1978 Jun;7(6):539–545. doi: 10.1128/jcm.7.6.539-545.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Smith P. B., Gavan T. L., Isenberg H. D., Sonnenwirth A., Taylor W. I., Washington J. A., 2nd, Balows A. Multi-laboratory evaluation of an automated microbial detection/identification system. J Clin Microbiol. 1978 Dec;8(6):657–666. doi: 10.1128/jcm.8.6.657-666.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Sonnenwirth A. C. Preprototype of an automated microbial detection and identification system: a developmental investigation. J Clin Microbiol. 1977 Oct;6(4):400–405. doi: 10.1128/jcm.6.4.400-405.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Warwood N. M., Blazevic D. J., Hofherr L. Comparison of the API 20E and Corning N/F systems for identification of nonfermentative gram-negative rods. J Clin Microbiol. 1979 Aug;10(2):175–179. doi: 10.1128/jcm.10.2.175-179.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]

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