Skip to main content
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1982 Feb;15(2):320–323. doi: 10.1128/jcm.15.2.320-323.1982

Use of chemotaxis chambers for studying in vitro bacterial colonization of biomaterials.

E S Leake, A G Gristina, M J Wright
PMCID: PMC272084  PMID: 7068827

Abstract

Blind-well chemotaxis chambers were used to study the in vitro bacterial adhesion and colonization of biomaterials. Staphylococcus aureus and Pseudomonas aeruginosa were selected for the bacterial inocula. Abundant growth on the surfaces of methyl methacrylate, polyethylene, stainless steel, and Vitallium was detected by using scanning electron microscopy after 24 h of incubation. The culture technique employed proved to be of value for the study of surface bacterial colonization of inert materials.

Full text

PDF
320

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Beveridge T. J. The response of cell walls of Bacillus subtilis to metals and to electron-microscopic stains. Can J Microbiol. 1978 Feb;24(2):89–104. doi: 10.1139/m78-018. [DOI] [PubMed] [Google Scholar]
  2. Costerton J. W., Geesey G. G., Cheng K. J. How bacteria stick. Sci Am. 1978 Jan;238(1):86–95. doi: 10.1038/scientificamerican0178-86. [DOI] [PubMed] [Google Scholar]
  3. Feldner J., Bredt W., Razin S. Adherence of Mycoplasma pneumoniae to glass surfaces. Infect Immun. 1979 Oct;26(1):70–75. doi: 10.1128/iai.26.1.70-75.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Geesey G. G., Richardson W. T., Yeomans H. G., Irvin R. T., Costerton J. W. Microscopic examination of natural sessile bacterial populations from an alpine stream. Can J Microbiol. 1977 Dec;23(12):1733–1736. doi: 10.1139/m77-249. [DOI] [PubMed] [Google Scholar]
  5. Gristina A. G., Rovere G. D., Shoji H., Nicastro J. F. An in vitro study of bacterial response to inert and reactive metals and to methyl methacrylate. J Biomed Mater Res. 1976 Mar;10(2):273–281. doi: 10.1002/jbm.820100208. [DOI] [PubMed] [Google Scholar]
  6. Hunter G., Dandy D. The natural history of the patient with an infected total hip replacement. J Bone Joint Surg Br. 1977 Aug;59(3):293–297. doi: 10.1302/0301-620X.59B3.893507. [DOI] [PubMed] [Google Scholar]
  7. Leake E. S., Wright M. J., Gristina A. G. A technique to study the bacterial colonization of surfaces employing chemotaxis chambers and scanning electron microscopy. Can J Microbiol. 1981 Nov;27(11):1228–1230. doi: 10.1139/m81-189. [DOI] [PubMed] [Google Scholar]
  8. Liu P. V., Shokrani F. Biological activities of pyochelins: iron-chelating agents of Pseudomonas aeruginosa. Infect Immun. 1978 Dec;22(3):878–890. doi: 10.1128/iai.22.3.878-890.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Marrie T. J., Lam J., Costerton J. W. Bacterial adhesion to uroepithelial cells: a morphologic study. J Infect Dis. 1980 Aug;142(2):239–246. doi: 10.1093/infdis/142.2.239. [DOI] [PubMed] [Google Scholar]
  10. Meares E. M., Stamey T. A. Bacteriologic localization patterns in bacterial prostatitis and urethritis. Invest Urol. 1968 Mar;5(5):492–518. [PubMed] [Google Scholar]
  11. Reynolds B. L., Pruul H. Protective role of smooth lipopolysaccharide in the serum bactericidal reaction. Infect Immun. 1971 Dec;4(6):764–771. doi: 10.1128/iai.4.6.764-771.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Weinberg E. D. Iron and susceptibility to infectious disease. Science. 1974 May 31;184(4140):952–956. doi: 10.1126/science.184.4140.952. [DOI] [PubMed] [Google Scholar]
  13. Weinberg E. D. Nutritional immunity. Host's attempt to withold iron from microbial invaders. JAMA. 1975 Jan 6;231(1):39–41. doi: 10.1001/jama.231.1.39. [DOI] [PubMed] [Google Scholar]
  14. Williams D. F., Meachim G. A combined metallurgical and histological study of tissue-prosthesis interactions in orthopedic patients. J Biomed Mater Res. 1974;8(3):1–9. doi: 10.1002/jbm.820080303. [DOI] [PubMed] [Google Scholar]
  15. Winter G. D. Tissue reactions to metallic wear and corrosion products in human patients. J Biomed Mater Res. 1974;8(3):11–26. doi: 10.1002/jbm.820080304. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES