Abstract
The N/F system (Flow Laboratories, Inc. Rockville, Md) and the Oxi/Ferm Tube, (Roche Diagnostics, Div. Hoffman-La Roche, Inc. Nutley, N.J.) were evaluated in parallel for identification of infrequently encountered nonfermentative gram-negative bacilli and oxidase-positive, fermentative gram-negative bacilli selected from fresh clinical isolates and stock cultures. When compared with conventional media, the Flow N/F system correctly identified 97.7% (86 or 88) of all strains tested. No organisms were misidentified, but this system failed to identify two strains. Six supplemental tests were needed for the complete identification of 16 strains (18%); however, correct results were obtained within 48 h for 85% of the isolates. The Oxi/Ferm method correctly identified 87.5% of the isolates; 7% were incorrectly identified, and the method failed to identify five strains. Seventeen supplemental tests were required to identify 64 strains (73%). Complete identification within 48 h was obtained for 60% of the organisms tested.
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Selected References
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- Appelbaum P. C., Stavitz J., Bentz M. S., von Kuster L. C. Four methods for identification of gram-negative nonfermenting rods: organisms more commonly encountered in clinical specimens. J Clin Microbiol. 1980 Aug;12(2):271–278. doi: 10.1128/jcm.12.2.271-278.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Barnishan J., Ayers L. W. Rapid identification of nonfermentative gram-negative rods by the Corning N/F system. J Clin Microbiol. 1979 Feb;9(2):239–243. doi: 10.1128/jcm.9.2.239-243.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dowda H. Evaluation of two rapid methods for identification of commonly encountered nonfermenting or oxidase-positive, Gram-negative rods. J Clin Microbiol. 1977 Dec;6(6):605–609. doi: 10.1128/jcm.6.6.605-609.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Nadler H., George H., Barr J. Accuracy and reproducibility of the Oxi/Ferm system in identifying a select group of unusual gram-negative bacilli. J Clin Microbiol. 1979 Feb;9(2):180–185. doi: 10.1128/jcm.9.2.180-185.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Nord C. E., Wretlind B., Dahlbäck A. Evaluation of two test-kits--API and Oxi Ferm tube--for identification of oxidative-fermentative Gram-negative rods. Med Microbiol Immunol. 1977 Jul 18;163(2):93–97. doi: 10.1007/BF02121824. [DOI] [PubMed] [Google Scholar]
- Oberhofer T. R. Comparison of the API 20E and Oxi/Ferm systems in identification of nonfermentative and oxidase-positive fermentative bacteria. J Clin Microbiol. 1979 Feb;9(2):220–226. doi: 10.1128/jcm.9.2.220-226.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Oberhofer T. R., Rowen J. W., Cunningham G. F., Higbee J. W. Evaluation of the oxi/ferm tube system with selected Gram-negative bacteria. J Clin Microbiol. 1977 Dec;6(6):559–566. doi: 10.1128/jcm.6.6.559-566.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Shayegani M., Lee A. M., McLynn D. M. Evaluation of the Oxi/Ferm tube system for identification of nonfermentative Gram-negative bacilli. J Clin Microbiol. 1978 Jun;7(6):533–538. doi: 10.1128/jcm.7.6.533-538.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Warwood N. M., Blazevic D. J., Hofherr L. Comparison of the API 20E and Corning N/F systems for identification of nonfermentative gram-negative rods. J Clin Microbiol. 1979 Aug;10(2):175–179. doi: 10.1128/jcm.10.2.175-179.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]