Abstract
Kovacs indole reagent, p-dimethylaminobenzaldehyde, Ehrlich indole reagent and p-dimethylaminocinnamaldehyde were used as spot indole reagents to test 359 strains of gram-negative rods growing on 5% sheep blood agar, Trypticase soy agar (BBL Microbiology Systems), and MacConkey agar. The p-dimethylaminocinnamaldehyde reagent was the most sensitive of those tested and provided results that were easiest to interpret. The p-dimethylaminocinnamaldehyde reagent was able to detect providencia alcalifaciens indole production because of the red-violet color unique to that organism. All reagents tested were accurate in detecting indole produced by members of the Enterobacteriaceae family, with the exception of P. alcalifaciens.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Dezfulian M., Dowell V. R., Jr Cultural and physiological characteristics and antimicrobial susceptibility of Clostridium botulinum isolates from foodborne and infant botulism cases. J Clin Microbiol. 1980 Jun;11(6):604–609. doi: 10.1128/jcm.11.6.604-609.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hicks M. J., Ryan K. J. Simplified scheme for identification of prompt lactose-fermenting members of the Enterobacteriaceae. J Clin Microbiol. 1976 Dec;4(6):511–514. doi: 10.1128/jcm.4.6.511-514.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lowrance B. L., Reich P., Traub W. H. Evaluation of two spot-indole reagents. Appl Microbiol. 1969 Jun;17(6):923–924. doi: 10.1128/am.17.6.923-924.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sutter V. L., Carter W. T. Evaluation of media and reagents for indole-spot tests in anaerobic bacteriology. Am J Clin Pathol. 1972 Sep;58(3):335–338. doi: 10.1093/ajcp/58.3.335. [DOI] [PubMed] [Google Scholar]