Abstract
Studies were performed to evaluate the use of the Enteric-Tek (ET) and the AutoMicrobic system (AMS) for the identification of 201 Enterobacteriaceae freshly isolated from clinical specimens. All test systems were inoculated simultaneously from the same MacConkey agar plate. Organisms were also identified with conventional media. Identifications with the ET and AMS agreed with those made with conventional biochemicals 97% of the time. At a 95% confidence level, the ET was able to identify 75% of the isolates within 18 h without the aid of additional biochemical tests; the AMS identified 92% in 8 h. Technologist time needed for identifications made with the AMS was reduced approximately 57% compared with the ET. In all instances in which the AMS identification disagreed with the conventional, the ET identified the organism correctly. Similarly, organisms misidentified by the ET were correctly identified by the AMS. The data suggest that the AMS and ET identify clinical isolates with comparable accuracy; however, the AMS offers a significant savings in time.
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Selected References
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- Aldridge C., Jones P. W., Gibson S., Lanham J., Meyer M., Vannest R., Charles R. Automated microbiological detection/identification system. J Clin Microbiol. 1977 Oct;6(4):406–413. doi: 10.1128/jcm.6.4.406-413.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Edberg S. C., Atkinson B., Chambers C., Moore M. H., Palumbo L., Zorzon C. F., Singer J. M. Clinical evaluation of the MICRO-ID, API 20E, and conventional media systems for identification of Enterobacteriacea. J Clin Microbiol. 1979 Aug;10(2):161–167. doi: 10.1128/jcm.10.2.161-167.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ferraro M. J., Edelblut M. A., Kunz L. J. Accurate automated identification of selected Enterobacteriaceae at four hours. J Clin Microbiol. 1981 Jan;13(1):151–157. doi: 10.1128/jcm.13.1.151-157.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hasyn J. J., Cundy K. R., Dietz C. C., Wong W. Clinical laboratory evaluation of the Auto-Microbic system for rapid identification of Enterobacteriaceae. J Clin Microbiol. 1981 Mar;13(3):491–497. doi: 10.1128/jcm.13.3.491-497.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Isenberg H. D., Gavan T. L., Smith P. B., Sonnenwirth A., Taylor W., Martin W. J., Rhoden D., Balows A. Collaborative investigation of the AutoMicrobic System Enterobacteriaceae biochemical card. J Clin Microbiol. 1980 Jun;11(6):694–702. doi: 10.1128/jcm.11.6.694-702.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Isenberg H. D., Gavan T. L., Sonnenwirth A., Taylor W. I., Washington J. A., 2nd Clinical laboratory evaluation of automated microbial detection/identification system in analysis of clinical urine specimens. J Clin Microbiol. 1979 Aug;10(2):226–230. doi: 10.1128/jcm.10.2.226-230.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Isenberg H. D., Scherber J. S., Cosgrove J. O. Clinical laboratory evaluation of the further improved Enterotube and Encise II. J Clin Microbiol. 1976 Aug;2(2):139–141. [PMC free article] [PubMed] [Google Scholar]
- Kelly M. T., Latimer J. M. Comparison of the automicrobic system with API, enterotube, micro-ID, micro-media systems, and conventional methods for identification of Enterobacteriaceae. J Clin Microbiol. 1980 Nov;12(5):659–662. doi: 10.1128/jcm.12.5.659-662.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rutherford I., Moody V., Gavan T. L., Ayers L. W., Taylor D. L. Comparative study of three methods of identification of Enterobacteriaceae. J Clin Microbiol. 1977 Apr;5(4):458–464. doi: 10.1128/jcm.5.4.458-464.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Smith P. B., Gavan T. L., Isenberg H. D., Sonnenwirth A., Taylor W. I., Washington J. A., 2nd, Balows A. Multi-laboratory evaluation of an automated microbial detection/identification system. J Clin Microbiol. 1978 Dec;8(6):657–666. doi: 10.1128/jcm.8.6.657-666.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sonnenwirth A. C. Preprototype of an automated microbial detection and identification system: a developmental investigation. J Clin Microbiol. 1977 Oct;6(4):400–405. doi: 10.1128/jcm.6.4.400-405.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]