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. 1982 May;15(5):824–829. doi: 10.1128/jcm.15.5.824-829.1982

Serological diagnosis of influenza B virus infection: comparison of an enzyme-linked immunosorbent assay and the hemagglutination inhibition test.

R Turner, J L Lathey, L P Van Voris, R B Belshe
PMCID: PMC272196  PMID: 7047553

Abstract

The sensitivity of an enzyme-linked immunosorbent assay (ELISA) for the detection of antibody to influenza B virus was compared with that of the hemagglutination inhibition test on acute- and convalescence-phase sera obtained from adults and children infected with influenza B virus. Two whole virus, tissue culture-grown antigen preparations were used in the ELISA, influenza B/West Virginia/81 and influenza B/Hong Kong/72. Four antigens were used in the hemagglutination inhibition test. These included the tissue culture-grown whole virus antigens that were used in the ELISA. In addition the standard egg-grown antigens, influenza B/Singapore/79 and influenza B/Hong Kong/72, were included for comparison. The ELISA antibody titer was significantly correlated to the hemagglutination inhibition antibody titer, and 10 of 10 adults and 17 of 21 children infected with influenza B had fourfold antibody increases as detected by ELISA with either antigenic type of tissue culture-grown whole virus. Increases in geometric mean antibody titers of 16- to 71-fold were detected by ELISA. Increases in geometric mean antibody titers of 3- to 10-fold were detected by hemagglutination inhibition depending on the type of antigen utilized. We found that ELISA with whole virus antigens was a sensitive and specific test for the detection of antibody to influenza B virus.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Belshe R. B., Van Voris L. P., Mufson M. A., Hyler L. Epidemiology of severe respiratory syncytial virus infections in Huntington, West Virginia. W V Med J. 1981 Mar;77(3):49–52. [PubMed] [Google Scholar]
  2. Bishai F. R., Galli R. Enzyme-linked immunosorbent assay for detection of antibodies to influenza A and B and parainfluenza type 1 in sera of patients. J Clin Microbiol. 1978 Dec;8(6):648–656. doi: 10.1128/jcm.8.6.648-656.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Coleman M. T., Dowdle W. R. Properties of the Hong Kong influenza virus. I. General characteristics of the Hong Kong virus. Bull World Health Organ. 1969;41(3):415–418. [PMC free article] [PubMed] [Google Scholar]
  4. Delia S., Russo V., Vullo V., Aceti A., Ferone U. Determination of specific antibodies to influenza by ELISA. Lancet. 1977 Jun 25;1(8026):1364–1364. doi: 10.1016/s0140-6736(77)92575-2. [DOI] [PubMed] [Google Scholar]
  5. Hammond G. W., Smith S. J., Noble G. R. Sensitivity and specificity of enzyme immunoassay for serodiagnosis of influenza A virus infections. J Infect Dis. 1980 May;141(5):644–651. doi: 10.1093/infdis/141.5.644. [DOI] [PubMed] [Google Scholar]
  6. KALTER S. S., CASEY H. L., JENSEN K. E., ROBINSON R. Q., GORRIE R. H. Evaluation of laboratory diagnostic procedures with A/Asian influenza. Proc Soc Exp Biol Med. 1959 Feb;100(2):367–370. doi: 10.3181/00379727-100-24629. [DOI] [PubMed] [Google Scholar]
  7. Ksiazek T. G., Olson J. G., Irving G. S., Settle C. S., White R., Petrusso R. An influenza outbreak due to A/USSR/77-like (H1N1) virus aboard a US Navy ship. Am J Epidemiol. 1980 Oct;112(4):487–494. doi: 10.1093/oxfordjournals.aje.a113018. [DOI] [PubMed] [Google Scholar]
  8. Lambre C., Kasturi K. N. A microplate immunoenzyme assay for anti-influenza antibodies. J Immunol Methods. 1979;26(1):61–67. doi: 10.1016/0022-1759(79)90041-3. [DOI] [PubMed] [Google Scholar]
  9. Masihi K. N., Lange W. Enzyme-linked immunosorbent assay for the detection of influenza type-specific antibodies. J Immunol Methods. 1980;36(2):173–179. doi: 10.1016/0022-1759(80)90041-1. [DOI] [PubMed] [Google Scholar]
  10. Monto A. S., Maassab H. F. Ether treatment of type B influenza virus antigen for the hemagglutination inhibition test. J Clin Microbiol. 1981 Jan;13(1):54–57. doi: 10.1128/jcm.13.1.54-57.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Murphy B. R., Phelan M. A., Nelson D. L., Yarchoan R., Tierney E. L., Alling D. W., Chanock R. M. Hemagglutinin-specific enzyme-linked immunosorbent assay for antibodies to influenza A and B viruses. J Clin Microbiol. 1981 Mar;13(3):554–560. doi: 10.1128/jcm.13.3.554-560.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Murphy B. R., Tierney E. L., Barbour B. A., Yolken R. H., Alling D. W., Holley H. P., Jr, Mayner R. E., Chanock R. M. Use of the enzyme-linked immunosorbent assay to detect serum antibody responses of volunteers who received attenuated influenza A virus vaccines. Infect Immun. 1980 Aug;29(2):342–347. doi: 10.1128/iai.29.2.342-347.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. RAPMUND G., JOHNSON R. T., BANKHEAD A. S., HERMAN Y. F., DANDRIDGE O. W. The diagnosis of Asian influenza virus infection after recent immunization. U S Armed Forces Med J. 1959 Jun;10(6):637–649. [PubMed] [Google Scholar]
  14. Richardson L. S., Yolken R. H., Belshe R. B., Camargo E., Kim H. W., Chanock R. M. Enzyme-linked immunosorbent assay for measurement of serological response to respiratory syncytial virus infection. Infect Immun. 1978 Jun;20(3):660–664. doi: 10.1128/iai.20.3.660-664.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Satz J., Prier J. E., Riley R., Schrack W. D., Jr, Hrehorovich V., Deardorff J. Epidemiological and biological characteristics of the Hong Kong influenza epidemic in Pennsylvania during 1968-1969. Am J Public Health Nations Health. 1970 Nov;60(11):2197–2207. doi: 10.2105/ajph.60.11.2197. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Schild G. C., Pereira M. S., Chakraverty P., Coleman M. T., Dowdle W. R., Chang W. K. Antigenic variants of influenza B virus. Br Med J. 1973 Oct 20;4(5885):127–131. doi: 10.1136/bmj.4.5885.127. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Van Voris L. P., Belshe R. B., Shaffer J. L. Nosocomial influenza B virus infection in the elderly. Ann Intern Med. 1982 Feb;96(2):153–158. doi: 10.7326/0003-4819-96-2-153. [DOI] [PubMed] [Google Scholar]
  18. Van Voris L. P., Betts R. F., Hayden F. G., Christmas W. A., Douglas R. G., Jr Successful treatment of naturally occurring influenza A/USSR/77 H1N1. JAMA. 1981 Mar 20;245(11):1128–1131. doi: 10.1001/jama.245.11.1128. [DOI] [PubMed] [Google Scholar]
  19. Wright P. F., Bryant J. D., Karzon D. T. Comparison of influenza B/Hong Kong virus infections among infants, children, and young adults. J Infect Dis. 1980 Apr;141(4):430–435. doi: 10.1093/infdis/141.4.430. [DOI] [PubMed] [Google Scholar]

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