Abstract
Sodium deoxycholate-extracted genus-specific chlamydial antigen was purified from contaminating substances by ion-exchange chromatography with DEAE-sephacel, resulting in a decrease in the complement-fixing activity of the antigen, whereas the enzyme-linked immunosorbent assay activity increased. By successive elution with 0.5, 1.0, and 1.5 M acetate buffer at least three clearly separated components were consistently recovered in 14 trials. The identity of these components as genus-specific chlamydial antigen was demonstrated in enzyme-linked immunosorbent assay tests with specific antisera. The antigenic activity of these components was not diminished by prior treatment of the chlamydial particles with pronase. Antiserum prepared by immunization of rabbits with the antigenic component I of an egg-propagated antigen reacted predominantly with the antigenic components I and II of a cell culture-propagated antigen.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- BENEDICT A. A., O'BRIEN E. Antigenic studies on the psittacosis-lymphogranuloma venereum group of viruses. II. Characterization of complement-fixing antigens extracted with sodium lauryl sulfate. J Immunol. 1956 Apr;76(4):293–300. [PubMed] [Google Scholar]
- Dhir S. P., Hakomori S., Kenny G. E., Grayston J. T. Immunochemical studies on chlamydial group antigen (presence of a 2-keto-3-deoxycarbohydrate as immunodominant group). J Immunol. 1972 Jul;109(1):116–122. [PubMed] [Google Scholar]
- Dhir S. P., Kenny G. E., Grayston J. T. Characterization of the group antigen of Chlamydia trachomatis. Infect Immun. 1971 Dec;4(6):725–730. doi: 10.1128/iai.4.6.725-730.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
- JENKIN H. M., ROSS M. R., MOULDER J. W. Species-specific antigens from the cell walls of the agents of meningopneumonitis and feline pneumonitis. J Immunol. 1961 Feb;86:123–127. [PubMed] [Google Scholar]
- Krauss H., Breinl M., Wachendörfer G., Valder W. A. Zur Anwendung des Agargel-Präzipitationstestes für die Diagnose von Chlamydieninfektionen bei Rind und Schaf. Berl Munch Tierarztl Wochenschr. 1975 Jan 1;88(1):1–4. [PubMed] [Google Scholar]
- Kronvall G. Purification of staphylococcal protein A using immunosorbents. Scand J Immunol. 1973;2(1):31–36. doi: 10.1111/j.1365-3083.1973.tb02013.x. [DOI] [PubMed] [Google Scholar]
- Schuurs A. H., Van Weemen B. K. Enzyme-immunoassay. Clin Chim Acta. 1977 Nov 15;81(1):1–40. doi: 10.1016/0009-8981(77)90410-7. [DOI] [PubMed] [Google Scholar]
- Sjöquist J., Movitz J., Johansson I. B., Hjelm H. Localization of protein A in the bacteria. Eur J Biochem. 1972 Oct 17;30(1):190–194. doi: 10.1111/j.1432-1033.1972.tb02086.x. [DOI] [PubMed] [Google Scholar]