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. 1982 May;15(5):873–878. doi: 10.1128/jcm.15.5.873-878.1982

Expanded serotyping scheme for Ureaplasma urealyticum strains isolated from humans.

J A Robertson, G W Stemke
PMCID: PMC272206  PMID: 7047554

Abstract

For the last decade the serological heterogeneity of strains of Ureaplasma urealyticum isolated from humans has been represented by eight serotypes. We have identified two additional serotypes, strains Vancouver and Western. Using a modified metabolic inhibition test and a colony indirect epifluorescence method, we tested antisera to the above 10 types against the 11 members of the independent Lin-Kass classification. Four of the latter, K2, U24, U26, and U38, showed unique specificities; these were confirmed by reciprocal testing with antisera prepared against the respective strains. With the addition of these strains, the serotyping scheme now has 14 members. Since this expanded classification allows greater discrimination between strains, it will allow also for their future characterization. It provides, in addition, a potentially more effective epidemiological tool.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Black F. T., Krogsgaard-Jensen A. Application of indirect immunofluorescence, indirect haemagglutination and polyacrylamide-gel electrophoresis to human T-mycoplasmas. Acta Pathol Microbiol Scand B Microbiol Immunol. 1974 Jun;82(3):345–353. doi: 10.1111/j.1699-0463.1974.tb02336.x. [DOI] [PubMed] [Google Scholar]
  2. Black F. T. Modifications of the growth inhibition test and its application to human T-mycoplasmas. Appl Microbiol. 1973 Apr;25(4):528–533. doi: 10.1128/am.25.4.528-533.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Cassell G. H., Cole B. C. Mycoplasmas as agents of human disease. N Engl J Med. 1981 Jan 8;304(2):80–89. doi: 10.1056/NEJM198101083040204. [DOI] [PubMed] [Google Scholar]
  4. Ford D. K., Henderson E. Serum antibody levels against T mycoplasmas in two North American Indian populations predisposed to spondylitis. Arthritis Rheum. 1976 Nov-Dec;19(6):1328–1332. doi: 10.1002/art.1780190614. [DOI] [PubMed] [Google Scholar]
  5. Graber C. D., Creticos P., Valicenti J., Williamson H. O. T mycoplasma in human reproductive failure. Obstet Gynecol. 1979 Nov;54(5):558–561. [PubMed] [Google Scholar]
  6. Lin J. S., Kass E. H. Fourteen serotypes of ureaplasma urealyticum (T-mycoplasmas) demonstrated by the complement-dependent mycoplasmacidal test. Infection. 1980;8(4):152–155. doi: 10.1007/BF01639122. [DOI] [PubMed] [Google Scholar]
  7. Lin J. S., Kass E. H. Serotypic heterogeneity in isolates of human genital T-mycoplasmas. Infect Immun. 1973 Mar;7(3):499–500. doi: 10.1128/iai.7.3.499-500.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Lin J. S., Kendrick M. I., Kass E. H. Serologic typing of human genital T-mycoplasmas by a complement-dependent mycoplasmacidal test. J Infect Dis. 1972 Dec;126(6):658–663. doi: 10.1093/infdis/126.6.658. [DOI] [PubMed] [Google Scholar]
  9. Piot P. Comparison of growth inhibition and immunofluorescence tests in serotyping clinical isolates of Ureaplasma urealyticum. Br J Vener Dis. 1977 Jun;53(3):186–189. doi: 10.1136/sti.53.3.186. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Purcell R. H., Taylor-Robinson D., Wong D., Chanock R. M. Color test for the measurement of antibody to T-strain mycoplasmas. J Bacteriol. 1966 Jul;92(1):6–12. doi: 10.1128/jb.92.1.6-12.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Quinn P. A., Arshoff L. U., Li H. C. Serotyping of Ureaplasma urealyticum by immunoperoxidase assay. J Clin Microbiol. 1981 Apr;13(4):670–676. doi: 10.1128/jcm.13.4.670-676.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Robertson J. A. Bromothymol blue broth: improved medium for detection of Ureaplasma urealyticum (T-strain mycoplasma). J Clin Microbiol. 1978 Feb;7(2):127–132. doi: 10.1128/jcm.7.2.127-132.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Robertson J. A., Stemke G. W. Modified metabolic inhibition test for serotyping strains of Ureaplasma urealyticum (T-strain Mycoplasma). J Clin Microbiol. 1979 Jun;9(6):673–676. doi: 10.1128/jcm.9.6.673-676.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. SHEPARD M. C., LUNCEFORD C. D. EFFECT OF PH ON HUMAN MYCOPLASMA STRAINS. J Bacteriol. 1965 Feb;89:265–270. doi: 10.1128/jb.89.2.265-270.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Shepard M. C., Lunceford C. D. Serological typing of Ureaplasma urealyticum isolates from urethritis patients by an agar growth inhibition method. J Clin Microbiol. 1978 Nov;8(5):566–574. doi: 10.1128/jcm.8.5.566-574.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Stemke G. W., Robertson J. A. Modified colony indirect epifluorescence test for serotyping Ureaplasma urealyticum and an adaptation to detect common antigenic specificity. J Clin Microbiol. 1981 Nov;14(5):582–584. doi: 10.1128/jcm.14.5.582-584.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Taylor-Robinson D., McCormack W. M. The genital mycoplasmas (first of two parts). N Engl J Med. 1980 May 1;302(18):1003–1010. doi: 10.1056/NEJM198005013021805. [DOI] [PubMed] [Google Scholar]

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