Abstract
The effectiveness of Micro-ID and API 20E as same-day identification systems for Enterobacteriaceae was evaluated in comparison with conventional identification by using 315 clinical isolates and 90 stock strains. The API 20E system was heavily inoculated according to manufacturer's recommendations for same-day identification. We found that 83 and 81% of isolates provided adequate inocula for Micro-ID and API 20E, respectively, and purity of the heavy inocula was not a problem with either system. Overall agreement with conventional identification at genus and species levels was 93.5% with Micro-ID and 90.2% with API 20E. However, when Klebsiella pneumoniae and K. oxytoca were considered as a single species and Proteus morganii was equated with Morganella morganii, agreement was 95.8 and 90.5%, respectively. Only 83.% of isolates were identified on the day of inoculation by API 20E, in contrast to 94.3% with Micro-ID. The remaining isolates required supplementary overnight testing. Provisional (low selectivity) determinations were constant with conventional identification with 49.3% of isolates with API 20E and 82.6% with Micro-ID. Telephone consultations with the manufacturers to resolve unprinted octal codes required a maximum of 15 min with Micro-ID and from 2 to greater than 48 h with API 20E.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Aldridge K. E., Gardner B. B., Clark S. J., Matsen J. M. Comparison of micro-ID, API 20E, and conventional media systems in identification of Enterobacteriaceae. J Clin Microbiol. 1978 Jun;7(6):507–513. doi: 10.1128/jcm.7.6.507-513.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Barry A. L., Badal R. E. Rapid identification of Enterobacteriaceae with the micro-ID system versus API 20E and conventional media. J Clin Microbiol. 1979 Sep;10(3):293–298. doi: 10.1128/jcm.10.3.293-298.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Edberg S. C., Atkinson B., Chambers C., Moore M. H., Palumbo L., Zorzon C. F., Singer J. M. Clinical evaluation of the MICRO-ID, API 20E, and conventional media systems for identification of Enterobacteriacea. J Clin Microbiol. 1979 Aug;10(2):161–167. doi: 10.1128/jcm.10.2.161-167.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Edberg S. C., Clare D., Moore M. H., Singer J. M. Rapid identification of Enterobacteriaceae from blood cultures with the Micro-ID system. J Clin Microbiol. 1979 Nov;10(5):693–697. doi: 10.1128/jcm.10.5.693-697.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gooch W. M., 3rd Evaluation of a multitest system for rapid identification of Salmonella and Shigella. Am J Clin Pathol. 1980 Apr;73(4):570–573. doi: 10.1093/ajcp/73.4.570. [DOI] [PubMed] [Google Scholar]