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. 1982 May;15(5):969–970. doi: 10.1128/jcm.15.5.969-970.1982

Comparison of direct and indirect immunofluorescence staining of clinical specimens for detection of respiratory syncytial virus antigen.

L L Minnich, C G Ray
PMCID: PMC272226  PMID: 7047562

Abstract

Immunofluorescence staining methods for respiratory syncytial virus antigen detection were compared. Of 50 specimens originally positive for respiratory syncytial virus by direct immunofluorescence and culture, 49 were positive by repeat direct immunofluorescence and 32 were positive by indirect immunofluorescence. Additional results obtained on specimens originally negative for respiratory syncytial virus by direct immunofluorescence, culture, or both indicate that direct immunofluorescence staining for respiratory syncytial virus antigen was more sensitive than was indirect immunofluorescence.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. D'Alessio D., Williams S., Dick E. C. Rapid detection and identification of respiratory viruses by direct immunofluorescence. Appl Microbiol. 1970 Aug;20(2):233–239. doi: 10.1128/am.20.2.233-239.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Fulton R. E., Middleton P. J. Comparison of immunofluorescence and isolation techniques in the diagnosis of respiratory viral infections of children. Infect Immun. 1974 Jul;10(1):92–101. doi: 10.1128/iai.10.1.92-101.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Knight V., Brasier F., Greenberg S. B., Jones D. B. Immunofluorescent diagnosis of acute viral infection. South Med J. 1975 Jun;68(6):764–766. doi: 10.1097/00007611-197506000-00027. [DOI] [PubMed] [Google Scholar]
  4. McQuillin J., Gardner P. S. Rapid diagnosis of respiratory syncytial virus infection by immunofluorescent antibody techniques. Br Med J. 1968 Mar 9;1(5592):602–605. doi: 10.1136/bmj.1.5592.602. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Minnich L., Ray C. G. Comparison of direct immunofluorescent staining of clinical specimens for respiratory virus antigens with conventional isolation techniques. J Clin Microbiol. 1980 Sep;12(3):391–394. doi: 10.1128/jcm.12.3.391-394.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]

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