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. 1982 Jun;15(6):1029–1035. doi: 10.1128/jcm.15.6.1029-1035.1982

Fractionation of Pneumocystis carinii antigens used in an enzyme-linked immunosorbent assay for antibodies and in the production of antiserum for detecting Pneumocystis carinii antigenemia.

S E Maddison, G V Hayes, M H Ivey, V C Tsang, S B Slemenda, L G Norman
PMCID: PMC272248  PMID: 6980894

Abstract

Cyst-rich suspensions of Pneumocystis carinii were obtained by differential and gradient centrifugation from heavily infected rat lungs. After preparation of an aqueous-soluble extract of the cyst-rich material, the insoluble residue was extracted with 8 M urea. Small amounts of infected human lung tissue and uninfected rat and human lung were processed similarly. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis showed that both human and rat infected lung extracts contained a large protein (greater than 200,000 daltons). This component was not present in extracts of uninfected lung. In addition, an HCl-soluble extract was prepared from the cyst-rich suspension from infected rat lung. The urea-extracted antigen was most reactive in an enzyme-linked immunosorbent assay. Rabbit antiserum against the HCl-soluble antigen detected circulating antigen in patients' sera in a counterimmunoelectrophoresis assay.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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