Abstract
The chromogenic cephalosporin nitrocefin was used to detect the presence of beta-lactamase in cerebrospinal fluid of patients suffering from ampicillin-resistant Haemophilus influenzae meningitis. Five samples of spinal fluid containing ampicillin-resistant isolates were studied, and all had beta-lactamase activity. When samples of spinal fluid containing 33 ampicillin-sensitive isolates and 234 sterile specimens were tested, no beta-lactamase activity was detected.
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Selected References
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- Bauer A. W., Kirby W. M., Sherris J. C., Turck M. Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol. 1966 Apr;45(4):493–496. [PubMed] [Google Scholar]
- Catlin B. W. Iodometric detection of Haemophilus influenzae beta-lactamase: rapid presumptive test for ampicillin resistance. Antimicrob Agents Chemother. 1975 Mar;7(3):265–270. doi: 10.1128/aac.7.3.265. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Jorgensen J. H., Alexander G. A. Comparative activities of selected beta-lactam antibiotics against Haemophilus influenzae. Antimicrob Agents Chemother. 1978 Feb;13(2):342–343. doi: 10.1128/aac.13.2.342. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lee W. S., Komarmy L. Iodometric spot test for detection of beta-lactamase in Haemophilus influenzae. J Clin Microbiol. 1981 Jan;13(1):224–225. doi: 10.1128/jcm.13.1.224-225.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Markowitz S. M. Isolation of an ampicillin-resistant, non-beta-lactamase-producing strain of Haemophilus influenzae. Antimicrob Agents Chemother. 1980 Jan;17(1):80–83. doi: 10.1128/aac.17.1.80. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Montgomery K., Raymundo L., Jr, Drew W. L. Chromogenic cephalosporin spot test to detect beta-lactamase in clinically significant bacteria. J Clin Microbiol. 1979 Feb;9(2):205–207. doi: 10.1128/jcm.9.2.205-207.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- O'Callaghan C. H., Morris A., Kirby S. M., Shingler A. H. Novel method for detection of beta-lactamases by using a chromogenic cephalosporin substrate. Antimicrob Agents Chemother. 1972 Apr;1(4):283–288. doi: 10.1128/aac.1.4.283. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Yolken R. H., Hughes W. T. Rapid diagnosis of infections caused by beta-lactamase-producing bacteria by means of an enzyme radioisotopic assay. J Pediatr. 1980 Nov;97(5):715–720. doi: 10.1016/s0022-3476(80)80252-6. [DOI] [PubMed] [Google Scholar]