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. 1982 Jun;15(6):1172–1175. doi: 10.1128/jcm.15.6.1172-1175.1982

Detection of "neutral" type 7F and type 14 pneumococcal capsular polysaccharides by immunoelectrophoresis.

S C Szu, L S Oravec
PMCID: PMC272277  PMID: 7107848

Abstract

Type 7F and type 14 pneumococcal capsular polysaccharides, neutral at pH 8.6, were studied by immunoelectrophoresis at pH 5. Three techniques were used: rocket, countercurrent, and reversed immunoelectrophoresis. Our results show that these two capsular polysaccharides types can be detected at pH 5 with high sensitivity.

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Selected References

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  1. Anhalt J. P., Yu P. K. Counterimmunoelectrophoresis of pneumococcal antigens:improved sensitivity for the detection of types VII and XIV. J Clin Microbiol. 1975 Dec;2(6):510–515. doi: 10.1128/jcm.2.6.510-515.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bjerrum O. J., Ingild A., Lowenstein H., Weeke B. Quantitation of human IgG by rocket immunoelectrophoresis at pH 5 by use of carbamylated antibodies. A routine laboratory method. Clin Chim Acta. 1973 Jul 14;46(3):337–343. doi: 10.1016/0009-8981(73)90189-7. [DOI] [PubMed] [Google Scholar]
  3. Coonrod J. D., Leach R. P. Immunoelectrophoresis for detection of polysaccharides in immune complexes. J Clin Microbiol. 1978 Aug;8(2):257–259. doi: 10.1128/jcm.8.2.257-259.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Coonrod J. D., Rytel M. W. Detection of type-specific pneumococcal antigens by counterimmunoelectrophoresis. I. Methodology and immunologic properties of pneumococcal antigens. J Lab Clin Med. 1973 May;81(5):770–777. [PubMed] [Google Scholar]
  5. Coonrod J. D., Rytel M. W. Detection of type-specific pneumococcal antigens by counterimmunoelectrophoresis. II. Etiologic diagnosis of pneumococcal pneumonia. J Lab Clin Med. 1973 May;81(5):778–786. [PubMed] [Google Scholar]
  6. Höyeraal H. M., Vandvik B., Mellbye O. J. Quantitation of diphtheria and tetanus antibodies by reversed rocket immunoelectrophoresis. J Immunol Methods. 1975 Feb;6(4):385–398. doi: 10.1016/0022-1759(75)90009-5. [DOI] [PubMed] [Google Scholar]
  7. Lee C. J., Pearson S. J., Robbins J. B. Quantitative determination of pneumococcal capsular polysaccharides in the polyvalent vaccine. I. Standardization of an immunoelectrophoretic method. J Biol Stand. 1980;8(4):271–280. doi: 10.1016/s0092-1157(80)80004-7. [DOI] [PubMed] [Google Scholar]
  8. Weeke B. Quantitative estimation of human immunoglobulins following carbamylation by electrophoresis in antibody-containing agarose. Scand J Clin Lab Invest. 1968;22(2):107–111. doi: 10.3109/00365516809160953. [DOI] [PubMed] [Google Scholar]

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