Abstract
Monoclonal antibodies which recognize the species-specific major outer membrane protein antigen of Chlamydia trachomatis were used for immunofluorescence staining of chlamydial inclusions in cell culture. A total of 115 clinical specimens were inoculated onto replicate HeLa 229 cell monolayers and assayed for chlamydial inclusions by immunofluorescence staining and Giemsa staining. Of the isolates, 38 were detected by immunofluorescence staining on passage 1 and 1 was detected on passage 2; 23 isolates on passage 1 and 13 isolates on passage 2 were detected by Giemsa staining. Immunofluorescence staining was significantly more sensitive than Giemsa staining for detecting chlamydial inclusions, particularly from specimens containing low titers of Chlamydia.
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- Alexander E. R., Wang S. P., Grayston J. T. Further classification of TRIC agents from ocular trachoma and other sources by the mouse toxicity prevention test. Am J Ophthalmol. 1967 May;63(5 Suppl):1469–1478. doi: 10.1016/0002-9394(67)94133-5. [DOI] [PubMed] [Google Scholar]
- BERNKOPF H., MASHIAH P., BECKER Y. Correlation between morphological and biochemical changes and the appearance of infectivity in FL cell cultures infected with trachoma agent. Ann N Y Acad Sci. 1962 Mar 5;98:62–81. doi: 10.1111/j.1749-6632.1962.tb30532.x. [DOI] [PubMed] [Google Scholar]
- Ey P. L., Prowse S. J., Jenkin C. R. Isolation of pure IgG1, IgG2a and IgG2b immunoglobulins from mouse serum using protein A-sepharose. Immunochemistry. 1978 Jul;15(7):429–436. doi: 10.1016/0161-5890(78)90070-6. [DOI] [PubMed] [Google Scholar]
- FURNESS G., GRAHAM D. M., REEVE P. The titration of trachoma and inclusion blennorrhoea viruses in cell cultures. J Gen Microbiol. 1960 Dec;23:613–619. doi: 10.1099/00221287-23-3-613. [DOI] [PubMed] [Google Scholar]
- Goding J. W. Conjugation of antibodies with fluorochromes: modifications to the standard methods. J Immunol Methods. 1976;13(3-4):215–226. doi: 10.1016/0022-1759(76)90068-5. [DOI] [PubMed] [Google Scholar]
- HANNA L., OKUMOTO M., THYGESON P., ROSE L., DAWSON C. R. TRIC AGENTS ISOLATED IN THE UNITED STATES. X. IMMUNOFLUORESCENCE IN THE DIAGNOSIS OF TRIC AGENT INFECTION IN MAN. Proc Soc Exp Biol Med. 1965 Jul;119:722–728. doi: 10.3181/00379727-119-30282. [DOI] [PubMed] [Google Scholar]
- Kuo C., Wang S., Wentworth B. B., Grayston J. T. Primary isolation of TRIC organisms in HeLa 229 cells treated with DEAE-dextran. J Infect Dis. 1972 Jun;125(6):665–668. doi: 10.1093/infdis/125.6.665. [DOI] [PubMed] [Google Scholar]
- Ripa K. T., Mårdh P. A. Cultivation of Chlamydia trachomatis in cycloheximide-treated mccoy cells. J Clin Microbiol. 1977 Oct;6(4):328–331. doi: 10.1128/jcm.6.4.328-331.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Stephens R. S., Tam M. R., Kuo C. C., Nowinski R. C. Monoclonal antibodies to Chlamydia trachomatis: antibody specificities and antigen characterization. J Immunol. 1982 Mar;128(3):1083–1089. [PubMed] [Google Scholar]
- Thomas B. J., Evans R. T., Hutchinson G. R., Taylor-Robinson D. Early detection of chlamydial inclusions combining the use of cycloheximide-treated McCoy cells and immunofluorescence staining. J Clin Microbiol. 1977 Sep;6(3):285–292. doi: 10.1128/jcm.6.3.285-292.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Woodland R. M., El-Sheikh H., Darougar S., Squires S. Sensitivity of immunoperoxidase and immunofluorescence staining for detecting chlamydia in conjunctival scrapings and in cell culture. J Clin Pathol. 1978 Nov;31(11):1073–1077. doi: 10.1136/jcp.31.11.1073. [DOI] [PMC free article] [PubMed] [Google Scholar]