Fig. 5.

AM and TSA inhibited cell proliferation and induced cell cycle arrest. (A) Growth arrest in HT29 cells treated with AM or TSA, detected using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Data = mean ± SD, n = 3, *P < 0.05. (B) DNA content as determined by flow cytometry (see Material and Methods). Results are representative of the findings from three independent experiments. For clarity, statistical outcomes associated with AM- or TSA-induced changes in G₁, S and G₂ cell cycle distribution versus vehicle control were omitted from the figure (*P < 0.05, all treatments).