Skip to main content
NCBI home page
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1982 Jul;16(1):63–69. doi: 10.1128/jcm.16.1.63-69.1982

Measurement of immunoglobulin G and M antibodies to type 3 pneumococcal capsular polysaccharide by enzyme-linked immunosorbent assay.

B A Carlson, G S Giebink, J S Spika, E D Gray
PMCID: PMC272295  PMID: 7107861

Abstract

An enzyme-linked immunosorbent assay was developed to measure immunoglobulin G (IgG) and IgM type 3 antipneumococcal capsular polysaccharide antibodies. The use of Fab2 fragments of rabbit antipneumococcal IgG antibody in the antibody-antigen sandwich increased the sensitivity for measuring IgM antibodies and decreased background activity in antigen-free cuvettes. This methodology detected type 3 IgM antibody responses in six of six subjects vaccinated with polyvalent pneumococcal vaccine and detected type 3 IgG antibody responses in three subjects. Results of the enzyme-linked immunosorbent assay and radioimmunoassay procedures were concordant, and postvaccination enzyme-linked immunosorbent assay IgM titers showed a stronger correlation with total radioimmunoassay antibody than did postvaccination ELISA IgG titers.

Full text

PDF
63

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ahlstedt S., Holmgren J., Hanson L. A. Protective capacity of antibodies against E. coli O antigen with special reference to the avidity. Int Arch Allergy Appl Immunol. 1974;46(3):470–480. doi: 10.1159/000231150. [DOI] [PubMed] [Google Scholar]
  2. Austrian R., Douglas R. M., Schiffman G., Coetzee A. M., Koornhof H. J., Hayden-Smith S., Reid R. D. Prevention of pneumococcal pneumonia by vaccination. Trans Assoc Am Physicians. 1976;89:184–194. [PubMed] [Google Scholar]
  3. Barrett D. J., Ammann A. J., Stenmark S., Wara D. W. Immunoglobulin G and M antibodies to pneumococcal polysaccharides detected by enzyme-linked immunosorbent assay. Infect Immun. 1980 Feb;27(2):411–417. doi: 10.1128/iai.27.2.411-417.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Butler J. E., Feldbush T. L., McGivern P. L., Stewart N. The enzyme-linked immunosorbent assay (ELISA): a measure of antibody concentration or affinity. Immunochemistry. 1978 Feb;15(2):131–136. doi: 10.1016/0161-5890(78)90053-6. [DOI] [PubMed] [Google Scholar]
  5. Callahan L. T., 3rd, Woodhour A. F., Meeker J. B., Hilleman M. R. Enzyme-linked immunosorbent assay for measurement of antibodies against pneumococcal polysaccharide antigens: comparison with radioimmunoassay. J Clin Microbiol. 1979 Oct;10(4):459–463. doi: 10.1128/jcm.10.4.459-463.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Denmark J. R., Chessum B. S. Standardization of enzyme-linked immunosorbent assay (ELISA) and the detection of Toxoplasma antibody. Med Lab Sci. 1978 Jul;35(3):227–232. [PubMed] [Google Scholar]
  7. Engvall E., Perlmann P. Enzyme-linked immunosorbent assay, Elisa. 3. Quantitation of specific antibodies by enzyme-labeled anti-immunoglobulin in antigen-coated tubes. J Immunol. 1972 Jul;109(1):129–135. [PubMed] [Google Scholar]
  8. Gerber M. A., Gray E. D., Ferrieri P., Kaplan E. L. Enzyme-linked immunosorbent assay of antibodies in human sera to streptococcal DNase B. J Lab Clin Med. 1980 Feb;95(2):258–265. [PubMed] [Google Scholar]
  9. Giebink G. S., Foker J. E., Kim Y., Schiffman G. Serum antibody and opsonic responses to vaccination with pneumococcal capsular polysaccharide in normal and splenectomized children. J Infect Dis. 1980 Mar;141(3):404–412. doi: 10.1093/infdis/141.3.404. [DOI] [PubMed] [Google Scholar]
  10. Giebink G. S., Verhoef J., Peterson P. K., Quie P. G. Opsonic requirements for phagocytosis of Streptococcus pneumoniae types VI, XVIII, XXIII, and XXV. Infect Immun. 1977 Nov;18(2):291–297. doi: 10.1128/iai.18.2.291-297.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Gray B. M. ELISA methodology for polysaccharide antigens: protein coupling of polysaccharides for adsorption to plastic tubes. J Immunol Methods. 1979;28(1-2):187–192. doi: 10.1016/0022-1759(79)90340-5. [DOI] [PubMed] [Google Scholar]
  12. Koskela M., Leinonen M. Comparison of ELISA and RIA for measurement of pneumococcal antibodies before and after vaccination with 14-valent pneumococcal capsular polysaccharide vaccine. J Clin Pathol. 1981 Jan;34(1):93–98. doi: 10.1136/jcp.34.1.93. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Mathiesen L. R., Feinstone S. M., Wong D. C., Skinhoej P., Purcell R. H. Enzyme-linked immunosorbent assay for detection of hepatitis A antigen in stool and antibody to hepatitis A antigen in sera: comparison with solid-phase radioimmunoassay, immune electron microscopy, and immune adherence hemagglutination assay. J Clin Microbiol. 1978 Feb;7(2):184–193. doi: 10.1128/jcm.7.2.184-193.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Menzel J., Jungfer H., Gemsa D. Contribution of immunoglobulins M and G, complement, and properdin to the intracellular killing of Escherichia coli by polymorphonuclear leukocytes. Infect Immun. 1978 Feb;19(2):659–666. doi: 10.1128/iai.19.2.659-666.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Schiffman G., Douglas R. M., Bonner M. J., Robbins M., Austrian R. A radioimmunoassay for immunologic phenomena in pneumococcal disease and for the antibody response to pneumococcal vaccines. I. Method for the radioimmunoassay of anticapsular antibodies and comparison with other techniques. J Immunol Methods. 1980;33(2):133–144. doi: 10.1016/s0022-1759(80)80004-4. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES