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. 1982 Jul;16(1):103–106. doi: 10.1128/jcm.16.1.103-106.1982

Comparison of paired whole milk and dried filter paper samples for anti-enterotoxin and anti-rotavirus activities.

S E Brown 3rd, K T Sauer, M Nations-Shields, D S Shields, J G Araujo, R L Guerrant
PMCID: PMC272303  PMID: 6286715

Abstract

Milk specimens, 75 from cows immunized against cholera toxin and 35 from a human population in which enterotoxigenic Escherichia coli and rotaviral infections are endemic, were collected as paired filter paper and frozen whole milk samples. Each pair was tested for antibody activity against heat-labile E. coli and Vibrio cholerae enterotoxins. Additionally, 12 of the 35 paired human milk samples stored as frozen whole milk and dried on filter paper were tested for anti-rotavirus immunoglobulin A. Anti-enterotoxin and anti-rotavirus immunoglobulin A titers in milk dried on filter paper compared favorably with those of their frozen whole milk pairs. Filter paper samples offered considerable advantages for field collection, transportation, and storage over frozen liquid samples.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Chandra R. K. Nutritional deficiency and susceptibility to infection. Bull World Health Organ. 1979;57(2):167–177. [PMC free article] [PubMed] [Google Scholar]
  2. Downham M. A., Scott R., Sims D. G., Webb J. K., Gardner P. S. Breast-feeding protects against respiratory syncytial virus infections. Br Med J. 1976 Jul 31;2(6030):274–276. doi: 10.1136/bmj.2.6030.274. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Goldsmith R. S., Kagan I. G., Zarate R., Reyes-González M. A., Cedeño-Ferreira J. Epidemiologic studies of Chagas' disease in Oaxaca, Mexico. Bull Pan Am Health Organ. 1978;12(3):236–250. [PubMed] [Google Scholar]
  4. Guerrant R. L., Brunton L. L. Characterization of the Chinese hamster ovary cell assay for the enterotoxins of Vibrio cholerae and Escherichia coli and for specific antisera, and toxoid. J Infect Dis. 1977 May;135(5):720–728. doi: 10.1093/infdis/135.5.720. [DOI] [PubMed] [Google Scholar]
  5. Lobel H. O., Nájera A. J., Ch'en W. I., Munroe P., Mathews H. M. Seroepidemiologic investigations of malaria in Guyana. J Trop Med Hyg. 1976 Dec;79(12):275–284. [PubMed] [Google Scholar]
  6. Mathews H. M., Spencer H. C., Healy G. R. Comparison of IHA test for amoebiasis on serum and filter paper specimens. Trans R Soc Trop Med Hyg. 1980;74(3):404–405. doi: 10.1016/0035-9203(80)90113-3. [DOI] [PubMed] [Google Scholar]
  7. McLean M., Brennan R., Hughes J. M., Korzeniowski O. M., de Souza M. A., Araujo J. G., Benevides T. M., Guerrant R. L. Etiology of childhood diarrhea and oral rehydration therapy in northeastern Brazil. Bull Pan Am Health Organ. 1981;15(4):318–325. [PubMed] [Google Scholar]
  8. Stoliar O. A., Pelley R. P., Kaniecki-Green E., Kkaus M. H., Carpenter C. C. Secretory IgA against enterotoxins in breast-milk. Lancet. 1976 Jun 12;1(7972):1258–1261. doi: 10.1016/s0140-6736(76)91735-9. [DOI] [PubMed] [Google Scholar]
  9. Yolken R. H., Wyatt R. G., Mata L., Urrutia J. J., Garciá B., Chanock R. M., Kapikian A. Z. Secretory antibody directed against rotavirus in human milk--measurement by means of enzyme-linked immunosorbent assay. J Pediatr. 1978 Dec;93(6):916–921. doi: 10.1016/s0022-3476(78)81211-6. [DOI] [PubMed] [Google Scholar]

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