Abstract
An enzyme-linked immunosorbent assay was developed for the titration of antibodies in human sera to influenza virus neuraminidase, employing partially purified N1 neuraminidase. Specificity of the test was demonstrated, and the test was more sensitive than either the conventional neuraminidase inhibition or plaque size reduction tests in detecting anti-neuraminidase antibody.
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- Aymard-Henry M., Coleman M. T., Dowdle W. R., Laver W. G., Schild G. C., Webster R. G. Influenzavirus neuraminidase and neuraminidase-inhibition test procedures. Bull World Health Organ. 1973;48(2):199–202. [PMC free article] [PubMed] [Google Scholar]
- Bishai F. R., Galli R. Enzyme-linked immunosorbent assay for detection of antibodies to influenza A and B and parainfluenza type 1 in sera of patients. J Clin Microbiol. 1978 Dec;8(6):648–656. doi: 10.1128/jcm.8.6.648-656.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bucher D. J., Kharitonenkov I. G., Zakomirdin J. A., Grigoriev V. B., Klimenko S. M., Davis J. F. Incorporation of influenza virus M-protein into liposomes. J Virol. 1980 Nov;36(2):586–590. doi: 10.1128/jvi.36.2.586-590.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bucher D. J., Li S. S., Kehoe J. M., Kilbourne E. D. Chromatographic isolation of the hemagglutinin polypeptides from influenza virus vaccine and determination of their amino-terminal sequences. Proc Natl Acad Sci U S A. 1976 Jan;73(1):238–242. doi: 10.1073/pnas.73.1.238. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Engvall E., Perlmann P. Enzyme-linked immunosorbent assay, Elisa. 3. Quantitation of specific antibodies by enzyme-labeled anti-immunoglobulin in antigen-coated tubes. J Immunol. 1972 Jul;109(1):129–135. [PubMed] [Google Scholar]
- Hammond G. W., Smith S. J., Noble G. R. Sensitivity and specificity of enzyme immunoassay for serodiagnosis of influenza A virus infections. J Infect Dis. 1980 May;141(5):644–651. doi: 10.1093/infdis/141.5.644. [DOI] [PubMed] [Google Scholar]
- Hierholzer J. C., Suggs M. T., Hall E. C. Standardized viral hemagglutination and hemagglutination-inhibition tests. II. Description and statistical evaluation. Appl Microbiol. 1969 Nov;18(5):824–833. doi: 10.1128/am.18.5.824-833.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Jahiel R. I., Kilbourne E. D. Reduction in plaque size and reduction in plaque number as differing indices of influenza virus-antibody reactions. J Bacteriol. 1966 Nov;92(5):1521–1534. doi: 10.1128/jb.92.5.1521-1534.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kendal A. P., Madeley C. R. Flocculation of influenza virus by specific anti-neuraminidase antibody. Arch Gesamte Virusforsch. 1970;31(3):219–229. doi: 10.1007/BF01253756. [DOI] [PubMed] [Google Scholar]
- Kendal A. P., Noble G. R., Dowdle W. R. Neuraminidase content of influenza vaccines and neuraminidase antibody responses after vaccination of immunologically primed and unprimed populations. J Infect Dis. 1977 Dec;136 (Suppl):S415–S424. doi: 10.1093/infdis/136.supplement_3.s415. [DOI] [PubMed] [Google Scholar]
- Kilbourne E. D., Christenson W. N., Sande M. Antibody response in man to influenza virus neuraminidase following influenza. J Virol. 1968 Jul;2(7):761–762. doi: 10.1128/jvi.2.7.761-762.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kolbourne E. D. Recombination of influenza A viruses of human and animal origin. Science. 1968 Apr 5;160(3823):74–76. doi: 10.1126/science.160.3823.74. [DOI] [PubMed] [Google Scholar]
- LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
- Murphy B. R., Phelan M. A., Nelson D. L., Yarchoan R., Tierney E. L., Alling D. W., Chanock R. M. Hemagglutinin-specific enzyme-linked immunosorbent assay for antibodies to influenza A and B viruses. J Clin Microbiol. 1981 Mar;13(3):554–560. doi: 10.1128/jcm.13.3.554-560.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Murphy B. R., Tierney E. L., Barbour B. A., Yolken R. H., Alling D. W., Holley H. P., Jr, Mayner R. E., Chanock R. M. Use of the enzyme-linked immunosorbent assay to detect serum antibody responses of volunteers who received attenuated influenza A virus vaccines. Infect Immun. 1980 Aug;29(2):342–347. doi: 10.1128/iai.29.2.342-347.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Richman D. D., Murphy B. R., Chanock R. M., Gwaltney J. M., Jr, Douglas R. G., Betts R. F., Blacklow N. R., Rose F. B., Parrino T. A., Levine M. M. Temperature-sensitive mutants of influenza A virus. XII. Safety, antigenicity, transmissibility, and efficacy of influenza A/Udorn/72-ts-1[E] recombinant viruses in human adults. J Infect Dis. 1976 Dec;134(6):585–594. [PubMed] [Google Scholar]
- Rott R., Becht H., Orlich M. The significance of influenza virus neuraminidase in immunity. J Gen Virol. 1974 Jan;22(1):35–41. doi: 10.1099/0022-1317-22-1-35. [DOI] [PubMed] [Google Scholar]
- Schulman J. L., Khakpour M., Kilbourne E. D. Protective effects of specific immunity to viral neuraminidase on influenza virus infection of mice. J Virol. 1968 Aug;2(8):778–786. doi: 10.1128/jvi.2.8.778-786.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]