Abstract
Objectives
It is important to assess the risk of static magnetic fields (SMFs) on human health, because epidemiological studies have indicated that SMFs play a role in the development of diseases such as leukemia and brain tumor. In our environment, we have numerous chances to be exposed to not only SMFs but also many chemicals containing mutagens. The aim of this study is to investigate the effect of SMFs on the induction of micronuclei induced by some mutagens.
Methods
BALB/c mice were exposed to 4.7 tesla (T) SMF for 24 hr immediately after the injection of carboquone (alkylating agent), colcemid (spindle poison), mitomycin C (cross-linking agent), vincristine (spindle poison), sodium fluoride (a byproduct of aluminum plants under strong SMF) or 1-ethyl-1-nitrosourea (brain tumor-, gliomas- and thymic lymphoma-inducing chemical).
Results
The frequency of micronuclei induced by six mutagens increased after co-exposure to SMF.
Conclusions
An additive/synergistic effect of SMF and chemicals was observed from the results of increased frequency of micronuclei induced by mutagens in mouse bone marrow erythrocytes.
Key words: micronuclei, magnetic fields, mitomycin C, vincristine, co-mutagenic
References
- (1).World Health Organization. Environmental health criteria for magnetic fields, 7, Human studies. Environmental health criteria 69, Magnetic fields, Geneva: World Health Organization; 1978; p. 107–118.
- (2).Suzuki Y, Ikehata M, Nakamura K, Nishioka M, Asamuma K, Koana T, Shimizu H. Induction of micronuclei in mice exposed to static magnetic fields. Mutagenesis. 2001; 427: 499–501. [DOI] [PubMed]
- (3).Ikehata M, Koana T, Suzuki Y, Shimizu H, Nakagawa M, Mutagenicity and co-mutagenicity of static magnetic fields detected by bacterial mutation assay. Mutat Res. 1999; 427: 147–156. [DOI] [PubMed]
- (4).Ikehata M, Takashima Y, Suzuki Y, Shimizu H, Miyakoshi J, Koana T. Exposure to a power frequency magnetic field (50 Hz, 40 mT) did not cause point mutation in bacteria. Environ Mutagen Res. 2001; 23: 215–222.
- (5).Schmid W. The micronucleus test for cytogenetic analysis. In: Hollaender A editor. Chemical Mutagenesis. New York: Plenum Press; 1976. p. 31–53.
- (6).Kastenbaum MA, Bowman KO. Tables for determining the statistical significance of mutation frequencies. Mutat Res. 1970; 9: 527–549. [DOI] [PubMed]
- (7).Tuji Y, Nakagawa N, Suzuki Y. Five-tesla static magnetic fields suppress food and water consumption and weight gain in mice. Ind Health. 1996; 34: 347–357. [DOI] [PubMed]
- (8).Gollapudi BB, Bruce RJ, Linscombe VA, Watanabe PG, Sinha AK. Effect of food and water deprivation on the parameters of the mouse bone marrow micronucleus test. Mutat Res. 1986; 173: 121–125. [DOI] [PubMed]
- (9).Fischman HK, Kelly DD, Rainer JD. Behavioral stress and sister chromatid exchanges. Environ Mutagen. 1985; 7 (S3): 51. [DOI]
- (10).Sato M, Bremner I. Oxygen free radicals and metallothionein. Free Radic Biol Med. 1993; 14: 325–337. [DOI] [PubMed]
- (11).Satoh M, Tsuji Y, Watanabe Y, Okonogi H, Suzuki Y, Nakagawa M, Shimizu H. Metallothionein content increased in the liver of mice exposed to magnetic fields. Arch Toxicol. 1997; 70: 315–318. [DOI] [PubMed]
- (12).Watanabe Y, Nakagawa M, Miyakoshi Y. Enhancement of lipid peroxidation in the liver mice exposed to magnetic fields. Ind Health. 1997; 35: 285–290. [DOI] [PubMed]
- (13).Suzuki Y, Nagae Y, Ishikawa T, Watanabe Y, Nagashima T, Matsukubo K, Shimizu H. Effect of erythropoietin on the micronucleus test. Environ Mol Mutagen. 1989; 13: 314–318. [DOI] [PubMed]
- (14).Milham S Jr. Mortality from leukemia in workers exposed to electrical and magnetic fields. N Engl J Med. 1982; 307: 249. [DOI] [PubMed]
- (15).Suzuki Y. The genotoxic effect of fluoride. Fluoride Res. 1991; 12: 1–13. (Article in Japanese)
- (16).Okonogi H, Nakagawa M, Tsuji Y. The effect of a 4.7 tesla static magnetic field on the frequency of micronucleated cells induced by mitomycin C. Tohoku J Exp Med. 1996; 180: 209–215. [DOI] [PubMed]
- (17).Nakahara T, Yaguchi H, Yoshida M, Miyakoshi J. Effect of exposure of CHO-K1 cells to a 10-T static magnetic field. Radiology. 2002; 224: 817–822. [DOI] [PubMed]
- (18).Takatsuji T, Sasaki MS, Takekoshi H. Effect of static magnetic field on the induction of chromosome aberrations by 4.9 MeV protons and 23 MeV alpha particles. J Rad Res. 1989; 30: 238–246. [DOI] [PubMed]
- (19).Koana T, Okada MO, Ikehata M, Nakagawa M. Increase in the mitotic recombination frequency inDrosophila melanogaster by magnetic field exposure and its suppression by vitamin E supplement. Mutat Res. 1997; 373: 55–60. [DOI] [PubMed]
- (20).Takashima Y, Miyakoshi JU, Ikehata M, Iwasaka M, Ueno S, Koana T. Genotoxic effect of strong static magnetic fields in DNA-rapair defective mutation ofDrosophila melanogaster. J Radiat Res. 2004; 45: 393–397. [DOI] [PubMed]
- (21).World Health Organization. Environmental health criteria 232, Static fields, Cellular and animal studies. Geneva: World Health Organization; 2006. p. 58–174.
- (22).Ding G, Yaguchi H, Yoshida M, Miyakoshi J. Increase in X-ray-induced mutations by exposure to mutagenic field (60Hz, 5 mT) in NF-κB-inhibited cells. Biochem Biophy Res Commun. 2000; 276: 238–243. [DOI] [PubMed]
- (23).miyakoshi J, Yoshida M, Shibuya K, Hiraoka M. Exposure to strong magnetic fields at power frequency potentiates x-ray-induced DNA strand brakes. J Radiat Res. 2000; 41: 293–302. [DOI] [PubMed]
- (24).Yaguchi H, Yoshida M, Ding G-R, Shingu K, Miyakoshi J. Increased chromatid-type chromosomal aberrations in mouse m5S cells exposed to power-line frequency magnetic fields. Int J Radiat Biol. 2000; 76: 1677–1684. [DOI] [PubMed]
- (25).Miyakoshi Y, Yoshikawa H, Toyama Y, Suzuki Y, Shimizu H. The frequencies of micronuclei induced by cisplatin in newborn rat astrocytes are increased by 50-Hz, 7.5- and 10-mT electromagnetic fields. Environ Health Prev Med. 2005; 10: 138–143. [DOI] [PMC free article] [PubMed]
- (26).Ansari RM, Hei TK. Effect of 60 Hz extremely low frequency magnetic fields (EMF) on radiation- and chemical-induced mutagenesis in mammalian cells. Carcinogenesis. 2000; 21: 1221–1226. [DOI] [PubMed]
- (27).Mandeville R, Franco E, Sidrac-Ghali S, Paris-Nadon L, Rocheleau N, Mercier G, Desy M, Devaux C, Gaboury L. Evaluation of the potential promoting effect of 60 Hz magnetic fields on N-ethyl-N-nitrosourea induced neurogenic tumors in F344 rats. Bioelectromagnetics. 2000; 1: 84–93. [DOI] [PubMed]