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. 1982 Aug;16(2):276–280. doi: 10.1128/jcm.16.2.276-280.1982

Quantitation of rabbit immunoglobulin G antibodies to Salmonella minnesota Re by enzyme-linked immunosorbent assay.

M Eskenazy, F Naumova, R Tekelieva, G Konstantinov
PMCID: PMC272344  PMID: 7119099

Abstract

An enzyme-linked immunosorbent assay was developed which allowed the measurement of rabbit immunoglobulin G antibodies directed to Salmonella minnesota Re glycolipid. Efficient adsorption of the antigen to polystyrene could only be effected provided it had been previously dialyzed against 0.2 M EDTA (pH 7.0) and subsequently treated with 0.2% sodium deoxycholate (15 min at 56 degrees C) in 0.05 M diethanolamine buffer (pH 9.6). The method is by far more sensitive than quantitative precipitation in the determination of IgG antibodies. Inhibition by glycolipid of binding S. minnesota Re antibodies to immobilized glycolipid attests to the specificity of the assay and permits the detection of as little as 100 ng of glycolipid.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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