Skip to main content
NCBI home page
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1982 Sep;16(3):417–421. doi: 10.1128/jcm.16.3.417-421.1982

Enzymatic profile of Pseudomonas maltophilia.

M O'Brien, G H Davis
PMCID: PMC272382  PMID: 6813350

Abstract

An enzymatic profile of 20 strains of Pseudomonas maltophilia was undertaken with conventional plate tests, API ZYM, and 4-methylumbelliferyl-conjugated substrates. All strains produced DNase, RNase, arbutinase, esterases and lipases, mucinase, acid and alkaline phosphatases, alkaline pyrophosphate diesterase, phosphoamidase, beta-glucosidase, leucine arylamidase, and acetatase and were hemolytic for horse, sheep, and rabbit blood. The majority of strains produced chitinase, hyaluronidase, albuminase, valine arylamidase, trypsin, alpha- and beta-glucosidases, and N-acetyl-beta-glucosaminidase. API ZYM and 4-methylumbelliferyl-conjugated substrate assays are rapid, simple, specific, and sensitive and may be useful as diagnostic aids in the identification of P. maltophilia and other pseudomonads.

Full text

PDF
417

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BARNETT J. A., INGRAM M., SWAIN T. The use of beta-glucosides in classifying yeasts. J Gen Microbiol. 1956 Dec;15(3):529–555. doi: 10.1099/00221287-15-3-529. [DOI] [PubMed] [Google Scholar]
  2. Bobey D. G., Ederer G. M. Rapid detection of yeast enzymes by using 4-methylumbelliferyl substrates. J Clin Microbiol. 1981 Feb;13(2):393–394. doi: 10.1128/jcm.13.2.393-394.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Carter G. R., Chengappa M. M. Hyaluronidase production by type B Pasteurella multocida from cases of hemorrhagic septicemia. J Clin Microbiol. 1980 Jan;11(1):94–96. doi: 10.1128/jcm.11.1.94-96.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Frank S. K., Gerber J. D. Hydrolytic enzymes of Moraxella bovis. J Clin Microbiol. 1981 Feb;13(2):269–271. doi: 10.1128/jcm.13.2.269-271.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Gilardi G. L. Pseudomonas species in clinical microbiology. Mt Sinai J Med. 1976 Nov-Dec;43(6):710–726. [PubMed] [Google Scholar]
  6. Hartman D. P., Murphy R. A. Production and detection of staphylococcal elastase. Infect Immun. 1977 Jan;15(1):59–65. doi: 10.1128/iai.15.1.59-65.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Holmes B., Lapage S. P., Easterling B. G. Distribution in clinical material and identification of Pseudomonas maltophilia. J Clin Pathol. 1979 Jan;32(1):66–72. doi: 10.1136/jcp.32.1.66. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Humble M. W., King A., Phillips I. API ZYM: a simple rapid system for the detection of bacterial enzymes. J Clin Pathol. 1977 Mar;30(3):275–277. doi: 10.1136/jcp.30.3.275. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. MYERS H. I., ROTH G. D. Hide powder and collagen lysis by organisms from the oral cavity. Oral Surg Oral Med Oral Pathol. 1956 Nov;9(11):1172–1184. doi: 10.1016/0030-4220(56)90187-6. [DOI] [PubMed] [Google Scholar]
  10. Maddocks J. L., Greenan M. J. A rapid method for identifying bacterial enzymes. J Clin Pathol. 1975 Aug;28(8):686–687. doi: 10.1136/jcp.28.8.686. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Nestle M., Roberts W. K. An extracellular nuclease from Serratia marcescens. I. Purification and some properties of the enzyme. J Biol Chem. 1969 Oct 10;244(19):5213–5218. [PubMed] [Google Scholar]
  12. Nord C. E., Sjöberg L., Wadström T., Wretlind B. Characterization of three Aeromonas and nine pseudomonas species by extracellular enzymes and haemolysins. Med Microbiol Immunol. 1975;161(2):79–87. doi: 10.1007/BF02121748. [DOI] [PubMed] [Google Scholar]
  13. Smith R. F., Willett N. P. Rapid plate method for screening hyaluronidase and chondroitin sulfatase-producing microorganisms. Appl Microbiol. 1968 Sep;16(9):1434–1436. doi: 10.1128/am.16.9.1434-1436.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. van der Wiel-Korstanje J. A., Winkler K. C. The faecal flora in ulcerative colitis. J Med Microbiol. 1975 Nov;8(4):491–501. doi: 10.1099/00222615-8-4-491. [DOI] [PubMed] [Google Scholar]
  15. von Graevenitz A. Clinical microbiology of unusual Pseudomonas species. Prog Clin Pathol. 1973;5:185–218. [PubMed] [Google Scholar]
  16. von Riesen V. L. Digestion of algin by Pseudomonas maltophilia and Pseudomonas putida. Appl Environ Microbiol. 1980 Jan;39(1):92–96. doi: 10.1128/aem.39.1.92-96.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES