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. 1982 Sep;16(3):473–477. doi: 10.1128/jcm.16.3.473-477.1982

Rapid diagnosis of rotavirus infection by direct detection of viral nucleic acid in silver-stained polyacrylamide gels.

A J Herring, N F Inglis, C K Ojeh, D R Snodgrass, J D Menzies
PMCID: PMC272392  PMID: 6182158

Abstract

A rapid simple technique for the diagnosis of rotavirus has been developed based on the sensitive detection of rotavirus double-stranded RNA genome segments separated in polyacrylamide gels. The method utilizes a recently described ultrasensitive silver stain for polypeptides, which can also detect subnanogram amounts of nucleic acid. The sensitivity of the technique is comparable with that of electron microscopy or enzyme-linked immunosorbent assay.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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