Skip to main content
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1982 Sep;16(3):590–592. doi: 10.1128/jcm.16.3.590-592.1982

Influence of a blood culture inoculation technique on detection of bacteremia by the BACTEC system.

L G Carlson, J J Plorde
PMCID: PMC272422  PMID: 6752196

Abstract

A 2-year study compared the influence of blood culture inoculation technique on the detection of bacteremia by an automated radiometric system (BACTEC; Johnston Laboratories, Inc.). A total of 4,690 specimens (20 ml each) were collected. Of each sample, 10 ml was inoculated into a pair of Bactec bottles at the bedside (BACTEC system). The remaining 10 ml was placed in an evacuated blood collection tube (VACUTAINER; Becton Dickinson VACUTAINER Systems) and transported to the laboratory for subsequent inoculation into an identical set of vials (VACUTAINER-BACTEC system). A total of 309 cultures grew organisms considered to be clinically significant. The recovery rate, time to positivity, and spectrum of isolates were similar for the two methods. There were substantially more sporeforming "contaminants" isolated in the VACUTAINER-BACTEC system.

Full text

PDF
590

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Eng J. Effect of sodium polyanethol sulfonate in blood cultures. J Clin Microbiol. 1975 Feb;1(2):119–123. doi: 10.1128/jcm.1.2.119-123.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Eng J., Holten E. Gelatin neutralization of the inhibitory effect of sodium polyanethol sulfonate on Neisseria meningitidis in blood culture media. J Clin Microbiol. 1977 Jul;6(1):1–3. doi: 10.1128/jcm.6.1.1-3.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Eng J., Iveland H. Inhibitory effect in vitro of sodium polyanethol sulfonate on the growth of Neisseria meningitidis. J Clin Microbiol. 1975 May;1(5):444–447. doi: 10.1128/jcm.1.5.444-447.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Graves M. H., Morello J. A., Kocka F. E. Sodium polyanethol sulfonate sensitivity of anaerobic cocci. Appl Microbiol. 1974 Jun;27(6):1131–1133. doi: 10.1128/am.27.6.1131-1133.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Rosner R. Comparison of recovery rates of various organisms from clinical hypertonic blood cultures by using various concentrations of sodium polyanethol sulfonate. J Clin Microbiol. 1975 Feb;1(2):129–131. doi: 10.1128/jcm.1.2.129-131.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Washington J. A., 2nd The microbiology of evacuated blood collection tubes. Ann Intern Med. 1977 Feb;86(2):186–188. doi: 10.7326/0003-4819-86-2-186. [DOI] [PubMed] [Google Scholar]
  7. Wilkins T. D., West S. E. Medium-dependent inhibition of Peptostreptococcus anaerobius by sodium polyanetholsulfonate in blood culture media. J Clin Microbiol. 1976 Apr;3(4):393–396. doi: 10.1128/jcm.3.4.393-396.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES