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. 1982 Oct;16(4):632–636. doi: 10.1128/jcm.16.4.632-636.1982

Primary culture media for routine urine processing.

J C Fung, B Lucia, E Clark, M Berman, J Goldstein, R F D'Amato
PMCID: PMC272435  PMID: 7153312

Abstract

It has been recommended that routine microbiological processing of urine specimens include quantitative plating onto blood agar medium along with a selective and differential agar such as MacConkey agar for gram-negative organisms. Few data have been published to justify this combination. To evaluate the validity of this recommendation 2,553 midstream, clean-voided urine samples were quantitatively plated onto blood agar, MacConkey agar, and colistin-nalidixic acid agar, which is a selective medium for gram-positive organisms. The amounts of growth on each of the three media were compared. Results indicated that the best medium combination was colistin-nalidixic acid agar and MacConkey agar. The use of colistin-nalidixic acid agar instead of blood agar increased the detection of significant growth of enterococci, lactobacilli, and Torulopsis glabrata.

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Selected References

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