Skip to main content
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1982 Oct;16(4):766–769. doi: 10.1128/jcm.16.4.766-769.1982

Determination of immunoglobulin A in saliva by immunobead enzyme-linked immunosorbent assay: comparison with single radial immunodiffusion.

D Bratthall, R P Ellen
PMCID: PMC272468  PMID: 6818248

Abstract

For the quantitative measurement of immunoglobulin A in human saliva, an immunobead enzyme-linked immunosorbent assay was investigated in detail. The method proved to be fast and sensitive, and it gave reproducible results. There was a strong correlation with results obtained by a single radial immunodiffusion method. The immunobead method gave 1.8 times higher values than the single radial immunodiffusion method with a lower correction factor for low-immunoglobulin A saliva and a higher factor for immunoglobulin A-rich saliva.

Full text

PDF
766

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Brandtzaeg P., Fjellanger I., Gjeruldsen S. T. Human secretory immunoglobulins. I. Salivary secretions from individuals with normal or low levels of serum immunoglobulins. Scand J Haematol Suppl. 1970;12:3–83. [PubMed] [Google Scholar]
  2. Hanson L. A., Andersson B., Carlsson B., Cruz J. R., Dahlgren U., Baltzer I. M., Edén C. S. The secretory IgA system and mucosal defence. Scand J Infect Dis Suppl. 1980;Suppl 24:13–21. [PubMed] [Google Scholar]
  3. Mancini G., Carbonara A. O., Heremans J. F. Immunochemical quantitation of antigens by single radial immunodiffusion. Immunochemistry. 1965 Sep;2(3):235–254. doi: 10.1016/0019-2791(65)90004-2. [DOI] [PubMed] [Google Scholar]
  4. Peppard J. V. Quantitative estimation of IgA in rats: a comparison of two methods. J Immunol Methods. 1979;31(1-2):129–139. doi: 10.1016/0022-1759(79)90293-x. [DOI] [PubMed] [Google Scholar]
  5. Sack D. A., Neogi P. K., Alam M. K. Immunobead enzyme-linked immunosorbent assay for quantitating immunoglobulin A in human secretions and serum. Infect Immun. 1980 Jul;29(1):281–283. doi: 10.1128/iai.29.1.281-283.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES