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. 1982 Nov;16(5):813–820. doi: 10.1128/jcm.16.5.813-820.1982

Detection of antibodies to influenza virus M protein by an enzyme-linked immunosorbent assay.

M W Khan, D J Bucher, A K Koul, G Kalish, H Smith, E D Kilbourne
PMCID: PMC272482  PMID: 6759527

Abstract

An enzyme-linked immunosorbent assay test system was developed in which purified influenza virus M protein was used for the detection of M antibody in human sera. Antibody levels to influenza A virus M protein were monitored in sera from a vaccine study population by using an enzyme-linked immunosorbent assay technique with purified M protein as the adsorbent antigen. A 10-fold variation in titers of preexisting M antibody was observed in this population of young adults. Increases of anti-M titer of 7- to 24-fold were observed upon immunization with Formalin-inactivated vaccine or after natural infection. The antibody response to M protein was dissociated from the response to the hemagglutinin or neuraminidase antigens. The M antibody response preceded or was coincident with the antibody response to H1 hemagglutinin upon natural exposure to circulating virus.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aymard-Henry M., Coleman M. T., Dowdle W. R., Laver W. G., Schild G. C., Webster R. G. Influenzavirus neuraminidase and neuraminidase-inhibition test procedures. Bull World Health Organ. 1973;48(2):199–202. [PMC free article] [PubMed] [Google Scholar]
  2. Bucher D. J., Kharitonenkov I. G., Zakomirdin J. A., Grigoriev V. B., Klimenko S. M., Davis J. F. Incorporation of influenza virus M-protein into liposomes. J Virol. 1980 Nov;36(2):586–590. doi: 10.1128/jvi.36.2.586-590.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bucher D. J., Li S. S., Kehoe J. M., Kilbourne E. D. Chromatographic isolation of the hemagglutinin polypeptides from influenza virus vaccine and determination of their amino-terminal sequences. Proc Natl Acad Sci U S A. 1976 Jan;73(1):238–242. doi: 10.1073/pnas.73.1.238. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cretescu L., Beare A. S., Schild G. C. Formation of antibody to matrix protein in experimental human influenza A virus infections. Infect Immun. 1978 Nov;22(2):322–327. doi: 10.1128/iai.22.2.322-327.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Hierholzer J. C., Suggs M. T., Hall E. C. Standardized viral hemagglutination and hemagglutination-inhibition tests. II. Description and statistical evaluation. Appl Microbiol. 1969 Nov;18(5):824–833. doi: 10.1128/am.18.5.824-833.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Khan M. W., Gallagher M., Bucher D., Cerini C. P., Kilbourne E. D. Detection of influenza virus neuraminidase-specific antibodies by an enzyme-linked immunosorbent assay. J Clin Microbiol. 1982 Jul;16(1):115–122. doi: 10.1128/jcm.16.1.115-122.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Kilbourne E. D. Genetic dimorphism in influenza viruses: characterization of stably associated hemagglutinin mutants differing in antigenicity and biological properties. Proc Natl Acad Sci U S A. 1978 Dec;75(12):6258–6262. doi: 10.1073/pnas.75.12.6258. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  9. Masihi K. N., Lange W. Enzyme-linked immunosorbent assay for the detection of influenza type-specific antibodies. J Immunol Methods. 1980;36(2):173–179. doi: 10.1016/0022-1759(80)90041-1. [DOI] [PubMed] [Google Scholar]
  10. Mostow S. R., Schild G. C., Dowdle W. R., Wood R. J. Application of the single radial diffusion test for assay of antibody to influenza type A viruses. J Clin Microbiol. 1975 Dec;2(6):531–540. doi: 10.1128/jcm.2.6.531-540.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Murphy B. R., Phelan M. A., Nelson D. L., Yarchoan R., Tierney E. L., Alling D. W., Chanock R. M. Hemagglutinin-specific enzyme-linked immunosorbent assay for antibodies to influenza A and B viruses. J Clin Microbiol. 1981 Mar;13(3):554–560. doi: 10.1128/jcm.13.3.554-560.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. O'Farrell P. H. High resolution two-dimensional electrophoresis of proteins. J Biol Chem. 1975 May 25;250(10):4007–4021. [PMC free article] [PubMed] [Google Scholar]
  13. Oxford J. S., Schild G. C. Immunological and physicochemical studies of influenza matrix (M) polypeptides. Virology. 1976 Oct 15;74(2):394–402. doi: 10.1016/0042-6822(76)90345-7. [DOI] [PubMed] [Google Scholar]
  14. Palese P., Ritchey M. B., Schulman J. L., Kilbourne E. D. Genetic composition of a high-yielding influenza A virus recombinant: a vaccine strain against "Swine" influenza. Science. 1976 Oct 15;194(4262):334–335. doi: 10.1126/science.968486. [DOI] [PubMed] [Google Scholar]
  15. Voller A., Bidwell D. E., Bartlett A. Enzyme immunoassays in diagnostic medicine. Theory and practice. Bull World Health Organ. 1976;53(1):55–65. [PMC free article] [PubMed] [Google Scholar]
  16. Webster R. G., Hinshaw V. S. Matrix protein from influenza A virus and its role in cross-protection in mice. Infect Immun. 1977 Sep;17(3):561–566. doi: 10.1128/iai.17.3.561-566.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]

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