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. 1982 Nov;16(5):826–831. doi: 10.1128/jcm.16.5.826-831.1982

Haemophilus influenzae: comparison of respiratory tract isolates with genitourinary tract isolates.

W L Albritton, J L Brunton, M Meier, M N Bowman, L A Slaney
PMCID: PMC272484  PMID: 6984048

Abstract

Haemophilus influenzae isolates recovered from the genitourinary (GU) tract were shown to have a significantly different biotype distribution compared with respiratory tract isolates. Biotype IV strains were recovered more commonly from the GU tract, and most strains were non-serotypable. Antibiotic-susceptible strains isolated from the GU tract more frequently harbored plasmids of less than 10 megadaltons than did antibiotic-susceptible respiratory tract strains. One 2.8-megadalton plasmid resident in a GU tract isolate and one 1.8-megadalton plasmid resident in a respiratory tract isolate were shown to be related to the small ampicillin resistance plasmids previously described in H. influenzae, Haemophilus parainfluenzae, Haemophilus ducreyi, and Neisseria gonorrhoeae. This supports the suggestion that these ampicillin resistance plasmids originated by transposition or recombination of the ampicillin transposon (TnA) with cryptic endogenous Haemophilus plasmids.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Albritton W. L., Brunton J. L., Slaney L., MacLean I. Plasmid-mediated sulfonamide resistance in Haemophilus ducreyi. Antimicrob Agents Chemother. 1982 Jan;21(1):159–165. doi: 10.1128/aac.21.1.159. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Albritton W. L., Hammond G. W., Ronald A. R. Bacteremic Haemophilus influenzae genitourinary tract infections in adults. Arch Intern Med. 1978 Dec;138(12):1819–1821. [PubMed] [Google Scholar]
  3. Albritton W. L., Penner S., Slaney L., Brunton J. Biochemical characteristics of Haemophilus influenzae in relationship to source of isolation and antibiotic resistance. J Clin Microbiol. 1978 Jun;7(6):519–523. doi: 10.1128/jcm.7.6.519-523.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Branefors P., Elgefors B., Olegård R. Intrauterine infection by non-capsulated Haemophilus influenzae in a case of maternal immunodeficiency. Scand J Infect Dis. 1980;12(1):70–73. doi: 10.3109/inf.1980.12.issue-1.13. [DOI] [PubMed] [Google Scholar]
  5. Brunton J. L., Maclean I., Ronald A. R., Albritton W. L. Plasmid-mediated ampicillin resistance in Haemophilus ducreyi. Antimicrob Agents Chemother. 1979 Feb;15(2):294–299. doi: 10.1128/aac.15.2.294. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Brunton J., Meier M., Ehrman N., Maclean I., Slaney L., Albritton W. L. Molecular epidemiology of beta-lactamase-specifying plasmids of Haemophilus ducreyi. Antimicrob Agents Chemother. 1982 Jun;21(6):857–863. doi: 10.1128/aac.21.6.857. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Courtney S. E., Hall R. T. Haemophilus influenzae sepsis in the premature infant. Am J Dis Child. 1978 Oct;132(10):1039–1040. doi: 10.1001/archpedi.1978.02120350107022. [DOI] [PubMed] [Google Scholar]
  8. DEMING J. E. Haemophilus influenzae vaginitis. Northwest Med. 1955 Sep;54(9):992–993. [PubMed] [Google Scholar]
  9. Dajani A. S., Asmar B. I., Thirumoorthi M. C. Systemic Haemophilus influenzae disease: an overview. J Pediatr. 1979 Mar;94(3):355–364. doi: 10.1016/s0022-3476(79)80571-5. [DOI] [PubMed] [Google Scholar]
  10. De Graaff J., Elwell L. P., Falkow S. Molecular nature of two beta-lactamase-specifying plasmids isolated from Haemophilus influenzae type b. J Bacteriol. 1976 Apr;126(1):439–446. doi: 10.1128/jb.126.1.439-446.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Denhardt D. T. A membrane-filter technique for the detection of complementary DNA. Biochem Biophys Res Commun. 1966 Jun 13;23(5):641–646. doi: 10.1016/0006-291x(66)90447-5. [DOI] [PubMed] [Google Scholar]
  12. Elwell L. P., De Graaff J., Seibert D., Falkow S. Plasmid-linked ampicillin resistance in haempohilus influenza type b. Infect Immun. 1975 Aug;12(2):404–410. doi: 10.1128/iai.12.2.404-410.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Elwell L. P., Saunders J. R., Richmond M. H., Falkow S. Relationships among some R plasmids found in Haemophilus influenzae. J Bacteriol. 1977 Jul;131(1):356–362. doi: 10.1128/jb.131.1.356-362.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Füzi M. Haemophili in sexually transmitted diseases. Lancet. 1980 Aug 30;2(8192):476–476. doi: 10.1016/s0140-6736(80)91910-8. [DOI] [PubMed] [Google Scholar]
  15. Halal F., Delorme L., Brazeau M., Ahronheim G. Congenital vesicular eruption caused by Haemophilus influenzae type b. Pediatrics. 1978 Oct;62(4):494–496. [PubMed] [Google Scholar]
  16. Hammerschlag M. R., Alpert S., Rosner I., Thurston P., Semine D., McComb D., McCormack W. M. Microbiology of the vagina in children: normal and potentially pathogenic organisms. Pediatrics. 1978 Jul;62(1):57–62. [PubMed] [Google Scholar]
  17. Heffron F., Bedinger P., Champoux J. J., Falkow S. Deletions affecting the transposition of an antibiotic resistance gene. Proc Natl Acad Sci U S A. 1977 Feb;74(2):702–706. doi: 10.1073/pnas.74.2.702. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Hirschmann J. V., Everett E. D. Haemophilus influenzae infections in adults: report of nine cases and a review of the literature. Medicine (Baltimore) 1979 Jan;58(1):80–94. doi: 10.1097/00005792-197901000-00005. [DOI] [PubMed] [Google Scholar]
  19. Laufs R., Kaulfers P. M., Jahn G., Teschner U. Molecular characterization of a small Haemophilus influenzae plasmid specifying beta-lactamase and its relationship to R factors from Neisseria gonorrhoeae. J Gen Microbiol. 1979 Mar;111(1):223–231. doi: 10.1099/00221287-111-1-223. [DOI] [PubMed] [Google Scholar]
  20. Laufs R., Riess F. C., Jahn G., Fock R., Kaulfers P. M. Origin of Haemophilus influenzae R factors. J Bacteriol. 1981 Aug;147(2):563–568. doi: 10.1128/jb.147.2.563-568.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Maniatis T., Jeffrey A., Kleid D. G. Nucleotide sequence of the rightward operator of phage lambda. Proc Natl Acad Sci U S A. 1975 Mar;72(3):1184–1188. doi: 10.1073/pnas.72.3.1184. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. McConaughy B. L., Laird C. D., McCarthy B. J. Nucleic acid reassociation in formamide. Biochemistry. 1969 Aug;8(8):3289–3295. doi: 10.1021/bi00836a024. [DOI] [PubMed] [Google Scholar]
  23. McCormack W. M., Evrard J. R., Laughlin C. F., Rosner B., Alpert S., Crockett V. A., McComb D., Zinner S. H. Sexually transmitted conditions among women college students. Am J Obstet Gynecol. 1981 Jan 15;139(2):130–133. doi: 10.1016/0002-9378(81)90433-6. [DOI] [PubMed] [Google Scholar]
  24. Meyers J. A., Sanchez D., Elwell L. P., Falkow S. Simple agarose gel electrophoretic method for the identification and characterization of plasmid deoxyribonucleic acid. J Bacteriol. 1976 Sep;127(3):1529–1537. doi: 10.1128/jb.127.3.1529-1537.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Norden C. W. Hemophilus influenzae infections in adults. Med Clin North Am. 1978 Sep;62(5):1037–1046. doi: 10.1016/s0025-7125(16)31753-9. [DOI] [PubMed] [Google Scholar]
  26. Ogden E., Amstey M. S. Hemophilus influenza septicemia and midtrimester abortion. J Reprod Med. 1979 Feb;22(2):106–108. [PubMed] [Google Scholar]
  27. Retter M. E., Bannatyne R. M. Neonatal infection with Haemophilus influenzae biotype III. Can Med Assoc J. 1980 Oct 18;123(8):717–718. [PMC free article] [PubMed] [Google Scholar]
  28. Roberts M. C., Smith A. L. Molecular characterization of "plasmid-free" antibiotic-resistant Haemophilus influenzae. J Bacteriol. 1980 Oct;144(1):476–479. doi: 10.1128/jb.144.1.476-479.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Roberts M., Elwell L. P., Falkow S. Molecular characterization of two beta-lactamase-specifying plasmids isolated from Neisseria gonorrhoeae. J Bacteriol. 1977 Aug;131(2):557–563. doi: 10.1128/jb.131.2.557-563.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Saunders J. R., Elwell L. P., Falkow S., Sykes R. B., Richmond M. H. beta-lactamases and R-plasmids of Haemophilus influenzae. Scand J Infect Dis Suppl. 1978;(13):16–22. [PubMed] [Google Scholar]
  31. Schildkraut C. Dependence of the melting temperature of DNA on salt concentration. Biopolymers. 1965;3(2):195–208. doi: 10.1002/bip.360030207. [DOI] [PubMed] [Google Scholar]
  32. Schuit K. E. Isolation of Haemophilus in urine cultures from children. J Pediatr. 1979 Oct;95(4):565–566. doi: 10.1016/s0022-3476(79)80769-6. [DOI] [PubMed] [Google Scholar]
  33. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  34. Speer M., Rosan R. C., Rudolph A. J. Hemophilus influenzae infection in the neonate mimicking respiratory distress syndrome. J Pediatr. 1978 Aug;93(2):295–296. doi: 10.1016/s0022-3476(78)80525-3. [DOI] [PubMed] [Google Scholar]
  35. Stuy J. H. Chromosomally integrated conjugative plasmids are common in antibiotic-resistant Haemophilus influenzae. J Bacteriol. 1980 Jun;142(3):925–930. doi: 10.1128/jb.142.3.925-930.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]

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