Abstract
Two methods of viable cell counts were evaluated for Mycoplasma hominis and Ureaplasma urealyticum: color change unit50 and colony-forming unit. The color change unit50 method gave higher estimates of cell numbers; furthermore, the color change unit50 values correlated better with the DNA content of the cell pellet and the published genome sizes.
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Selected References
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- Bak A. L., Black F. T., Christiansen C., Freundt E. A. Genome size of mycoplasmal DNA. Nature. 1969 Dec 20;224(5225):1209–1210. doi: 10.1038/2241209a0. [DOI] [PubMed] [Google Scholar]
- Bode H. R., Morowitz H. J. Size and structure of the Mycoplasma hominis H39 chromosome. J Mol Biol. 1967 Jan 28;23(2):191–199. doi: 10.1016/s0022-2836(67)80026-3. [DOI] [PubMed] [Google Scholar]
- Ford D. K. Inhibition of growth of T-strain mycoplasmas by hydroxamic acids and by aurothiomalate. Antimicrob Agents Chemother. 1972 Nov;2(5):340–343. doi: 10.1128/aac.2.5.340. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Masover G. K., Mischak R. P., Hayflick L. Some effects of growth medium composition on the antigenicity of a T-strain mycoplasma. Infect Immun. 1975 Mar;11(3):530–539. doi: 10.1128/iai.11.3.530-539.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mohadjer S., Kaftarians S. The interaction between Mycoplasma hominis and poliovirus in cell culture. J Gen Virol. 1973 Sep;20(3):303–310. doi: 10.1099/0022-1317-20-3-303. [DOI] [PubMed] [Google Scholar]
- Purcell R. H., Taylor-Robinson D., Wong D., Chanock R. M. Color test for the measurement of antibody to T-strain mycoplasmas. J Bacteriol. 1966 Jul;92(1):6–12. doi: 10.1128/jb.92.1.6-12.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Razin S. The mycoplasmas. Microbiol Rev. 1978 Jun;42(2):414–470. doi: 10.1128/mr.42.2.414-470.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Richards G. M. Modifications of the diphenylamine reaction giving increased sensitivity and simplicity in the estimation of DNA. Anal Biochem. 1974 Feb;57(2):369–376. doi: 10.1016/0003-2697(74)90091-8. [DOI] [PubMed] [Google Scholar]
- Robertson J. A. Bromothymol blue broth: improved medium for detection of Ureaplasma urealyticum (T-strain mycoplasma). J Clin Microbiol. 1978 Feb;7(2):127–132. doi: 10.1128/jcm.7.2.127-132.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Robertson J., Gomersall M., Gill P. Mycoplasma hominis: growth, reproduction, and isolation of small viable cells. J Bacteriol. 1975 Nov;124(2):1007–1018. doi: 10.1128/jb.124.2.1007-1018.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Seidler R. J., Mandel M. Quantitative aspects of deoxyribonucleic acid renaturation: base composition, state of chromosome replication, and polynucleotide homologies. J Bacteriol. 1971 May;106(2):608–614. doi: 10.1128/jb.106.2.608-614.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]