Abstract
We developed a sensitive peroxidase staining procedure to identify oligoclonal band specificity in subacute sclerosing panencephalitis by subjecting serum and cerebrospinal fluid to isoelectric focusing and immunofixation with measles virus. The gel was washed and stained with horseradish peroxidase-conjugated goat antihuman immunoglobulin G. The samples showed dark brown measles-specific oligoclonal bands in the alkaline pH region of the gel, whereas the controls showed no significant staining. This technique may be useful in identifying specific antibody activity against viral and other antigens in oligoclonal bands in cerebrospinal fluid and serum.
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- Kinnman J., Link H., Frydén A. Characterization of antibody activity in oligoclonal immunoglobulin G synthesized within the central nervous system in a patient with tuberculous meningitis. J Clin Microbiol. 1981 Jan;13(1):30–35. doi: 10.1128/jcm.13.1.30-35.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lin F. H., Mehta P. D., Thormar H. Precipitation of measles virus proteins by immunoglobulin G fractions containing groups of oligoclonal bands isolated from sera of patients with subacute sclerosing panencephalitis. Infect Immun. 1982 Jul;37(1):393–396. doi: 10.1128/iai.37.1.393-396.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mehta P. D., Kane A., Thormar H. Quantitation of measles virus-specific immunoglobulins in serum, CSF, and brain extract from patients with subactue sclerosing panencephalitis. J Immunol. 1977 Jun;118(6):2254–2261. [PubMed] [Google Scholar]
- Mehta P. D., Lassmann H., Wisniewski H. M. Immunologic studies of chronic relapsing EAE in guinea pigs: similarities to multiple sclerosis. J Immunol. 1981 Jul;127(1):334–338. [PubMed] [Google Scholar]
- Mehta P. D., Patrick B. A., Thormar H. Measles-specific IgG in CSF and serum from patients with subacute sclerosing panencephalitis. Immunology. 1982 Jun;46(2):423–428. [PMC free article] [PubMed] [Google Scholar]
- Mehta P. D., Patrick B. A., Wisniewski H. M. Isoelectric focusing and immunofixation of cerebrospinal fluid and serum in multiple sclerosis. J Clin Lab Immunol. 1981 Jul;6(1):17–22. [PubMed] [Google Scholar]
- Nordal H. J., Vandvik B., Norrby E. Demonstration of electrophoretically restricted virus-specific antibodies in serum and cerebrospinal fluid by imprint electroimmunofixation. Scand J Immunol. 1978;7(5):381–388. doi: 10.1111/j.1365-3083.1978.tb00468.x. [DOI] [PubMed] [Google Scholar]
- Vandvik B., Norrby E., Nordal H. J., Degré M. Oligoclonal measles virus-specific IgG antibodies isolated from cerebrospinal fluids, brain extracts, and sera from patients with subacute sclerosing panencephalitis and multiple sclerosis. Scand J Immunol. 1976;5(8):979–992. doi: 10.1111/j.1365-3083.1976.tb03050.x. [DOI] [PubMed] [Google Scholar]
- Vandvik B., Norrby E. Oligoclonal IgG antibody response in the central nervous system to different measles virus antigens in subacute sclerosing panencephalitis. Proc Natl Acad Sci U S A. 1973 Apr;70(4):1060–1063. doi: 10.1073/pnas.70.4.1060. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Vandvik B., Norrby E., Steen-Johnsen J., Stensvold K. Mumps meningitis: prolonged pleocytosis and occurrence of mumps virus-specific oligoclonal IgG in the cerebrospinal fluid. Eur Neurol. 1978;17(1):13–22. doi: 10.1159/000114916. [DOI] [PubMed] [Google Scholar]
- Vandvik B., Weil M. L., Grandien M., Norrby E. Progressive rubella virus panencephalitis: synthesis of oligoclonal virus-specific IgG antibodies and homogeneous free light chains in the central nervous system. Acta Neurol Scand. 1978 Jan;57(1):53–64. doi: 10.1111/j.1600-0404.1978.tb04497.x. [DOI] [PubMed] [Google Scholar]