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. 2009 Aug 28;5(8):e1000618. doi: 10.1371/journal.pgen.1000618

The Genome of Nectria haematococca: Contribution of Supernumerary Chromosomes to Gene Expansion

Jeffrey J Coleman 1,2,#, Steve D Rounsley 3,#, Marianela Rodriguez-Carres 1,4,#, Alan Kuo 5, Catherine C Wasmann 1, Jane Grimwood 6,7, Jeremy Schmutz 6,7, Masatoki Taga 8, Gerard J White 1, Shiguo Zhou 9, David C Schwartz 9, Michael Freitag 10, Li-jun Ma 11, Etienne G J Danchin 12,13, Bernard Henrissat 12, Pedro M Coutinho 12, David R Nelson 14, Dave Straney 15, Carolyn A Napoli 1, Bridget M Barker 1, Michael Gribskov 16, Martijn Rep 17, Scott Kroken 1, István Molnár 18, Christopher Rensing 19, John C Kennell 20, Jorge Zamora 1, Mark L Farman 21, Eric U Selker 22, Asaf Salamov 5, Harris Shapiro 5, Jasmyn Pangilinan 5, Erika Lindquist 5, Casey Lamers 9, Igor V Grigoriev 5, David M Geiser 23, Sarah F Covert 24, Esteban Temporini 1,25, Hans D VanEtten 1,*
Editor: Hiten D Madhani26
PMCID: PMC2725324  PMID: 19714214

Abstract

The ascomycetous fungus Nectria haematococca, (asexual name Fusarium solani), is a member of a group of >50 species known as the “Fusarium solani species complex”. Members of this complex have diverse biological properties including the ability to cause disease on >100 genera of plants and opportunistic infections in humans. The current research analyzed the most extensively studied member of this complex, N. haematococca mating population VI (MPVI). Several genes controlling the ability of individual isolates of this species to colonize specific habitats are located on supernumerary chromosomes. Optical mapping revealed that the sequenced isolate has 17 chromosomes ranging from 530 kb to 6.52 Mb and that the physical size of the genome, 54.43 Mb, and the number of predicted genes, 15,707, are among the largest reported for ascomycetes. Two classes of genes have contributed to gene expansion: specific genes that are not found in other fungi including its closest sequenced relative, Fusarium graminearum; and genes that commonly occur as single copies in other fungi but are present as multiple copies in N. haematococca MPVI. Some of these additional genes appear to have resulted from gene duplication events, while others may have been acquired through horizontal gene transfer. The supernumerary nature of three chromosomes, 14, 15, and 17, was confirmed by their absence in pulsed field gel electrophoresis experiments of some isolates and by demonstrating that these isolates lacked chromosome-specific sequences found on the ends of these chromosomes. These supernumerary chromosomes contain more repeat sequences, are enriched in unique and duplicated genes, and have a lower G+C content in comparison to the other chromosomes. Although the origin(s) of the extra genes and the supernumerary chromosomes is not known, the gene expansion and its large genome size are consistent with this species' diverse range of habitats. Furthermore, the presence of unique genes on supernumerary chromosomes might account for individual isolates having different environmental niches.

Author Summary

Nectria haematococca MPVI occurs as a saprophyte in diverse habitats and as a plant and animal pathogen. It also was the first fungus shown to contain supernumerary chromosomes with unique habitat-defining genes. The current study reveals that it has one of the largest fungal genomes (15,707 genes), which may be related to its habitat diversity, and describes two additional supernumerary chromosomes. Two classes of genes were identified that have contributed to gene expansion: 1) specific genes that are not found in other fungi, and 2) genes that are present as multiple copies in N. haematococca but commonly occur as a single copy in other fungi. Some of these genes have properties suggesting their acquisition by horizontal gene transfer. We show that the three supernumerary chromosomes are different from the normal chromosomes; they contain more repeat sequences, are particularly enriched in unique and duplicated genes, and have a lower G+C content. Additionally, the biochemical functions of genes on these chromosomes suggest they may be involved in niche adaptation. The dispensable nature and possession of habitat-determining genes by these chromosomes make them the biological equivalent of bacterial plasmids. We believe they contribute to microbial diversity and have been overlooked in models of fungal evolution.

Introduction

The fungus Nectria haematococca, commonly referred to by its asexual name Fusarium solani, is a member of a monophyletic clade that includes over 50 phylogenetic species known as the “Fusarium solani species complex” [1],[2]. Members of the F. solani species complex are able to colonize an impressive variety of environments. As saprobes, they are present in agricultural and non-cultivated habitats, such as forests, scrub communities, savannahs, prairies, swamps, littoral zones, coastal zones, and deserts [3]. As pathogens, members of this complex are responsible for disease on ∼100 genera of plants [4], and they represent one of the most important group of pathogens associated with opportunistic fungal infections and keratitis in humans [2], [5]–[8]. Because of their diverse host range, some members have been proposed for the biological control of weeds and other pathogens [9]–[11]. Extreme environments are not beyond the reach of these fungi. F. solani is among the fungal species recovered from the highly radioactive inner parts of the damaged nuclear reactor at Chernobyl [12]. These fungi are capable of growing in anaerobic conditions in the soil [13] and are tolerant to many compounds shown to be toxic to other fungi [14],[15]. F. solani also has been found growing in the caves at Lascaux, France where it is damaging the 15,000 year-old paintings [16]. The ability of these fungi to adapt to so many different environments reflects their genetic plasticity and metabolic diversity. Individual members of this species complex can degrade hydrocarbons, organofluorine compounds, lignin, metal cyanides, and pesticides in the soil [17]–[29].

The most extensively studied species of the F. solani complex is “mating population” (MP) VI of N. haematococca (also called Haematonectria haematococca [30]). The term “mating population” defines a group of isolates that are sexually fertile with one another, indicating that they are a biological species. Like other F. solani species, N. haematococcaa MPVI isolates can live in many habitats [31] and classical and molecular genetic analyses have demonstrated that the genes controlling the ability of individual isolates to colonize specific habitats are located on conditionally-dispensable supernumerary chromosomes (“CD chromosomes”), which were first described in fungi in 1991 using N. haematococca MPVI [32]. CD chromosomes are defined as supernumerary chromosomes that are not required for growth under all conditions but confer an adaptive advantage in certain habitats [33]. Subsequent research has demonstrated that in N. haematococca MPVI, genes on these chromosomes are involved in resistance to plant antimicrobials, utilization of specific carbon and nitrogen sources, and in host-specific pathogenicity [33]–[35]. In addition, the properties of the chromosomes and the properties of the genes on these chromosomes, suggest that some of these genes and perhaps even the entire chromosome(s) might have been acquired through horizontal gene transfer (HGT) and have properties similar to the genomic islands of bacteria [36]. The genomic sequence of N. haematococca MPVI has not only the potential to reveal a multitude of metabolic pathways involved in inhabiting many different types of environments, but also to expand on our understanding of the impact of gene flow on fungal evolution.

Results

General genome features

Optical mapping revealed that N. haematococca MPVI isolate 77-13-4 has 17 chromosomes ranging from 530 kb to 6.52 Mb and that the physical size of the genome, 54.43 Mb, is larger than that of any other published ascomycete (Table S1). This is 15% greater than that of the most closely related sequenced fungus, Fusarium graminearum (sexual name: Gibberella zeae), which is known to have undergone significant gene expansion itself [37]. The average gene length (1.67 kb), number of exons (3.08), intron size (84 nt), and the size of the encoded protein (480 aa) (Table S2) are similar to other sequenced ascomycetes [38],[39]. Of the gene families in N. haematococca MPVI with at least ten genes, 77% (226 of 293) have more genes than their counterpart in F. graminearum; 18% have more than twice as many genes (Figure S1). As might be expected for a metabolically diverse fungus that can live in so many habitats, among the gene families with the greatest numerical increases are carbohydrate-active enzymes, oxidoreductases, and various monooxygenases and dioxygenases (Table S3).

Chromosomal location of genes similar to other fungi

To determine why N. haematococca MPVI might have more genes than F. graminearum and to see if these “extra” genes are similar to genes from other fungi, the proteome of N. haematococca MPVI was compared to the genomes of eight other sequenced fungi (F. graminearum, Aspergillus oryzae, A. nidulans, Coccidioides immitis, Chaetomium globosum, Magnaporthe oryzae, Neurospora crassa, and Saccharomyces cerevisiae). The predicted genes of N. haematococca MPVI were classified into three groups: those most similar to F. graminearum, those most similar to genes from the other fungi used in the comparison, or those with no similarity to genes from any of the included genomes. 61.5% were most similar to F. graminearum genes, 28.5% were more similar to the genes from other fungi, and 6.4% had no good match with any of the other genomes. Among the genes with the highest similarity outside F. graminearum, the highest similarity was to Aspergillus species (1786 genes), particularly to A. oryzae (811 genes).

The percentage of the genes in each category also was determined for each chromosome. With the exception of chromosome 7, the majority (>60%) of the genes on the large chromosomes (chromosomes 1–10; ranging in size from 6.52 to 3.00 Mb) are highly similar to genes found in F. graminearum (Figure 1), suggesting that these chromosomes are largely derived from an ancestor common to both N. haematococca MPVI and F. graminearum. For chromosomes 7 (3.83 Mb) and 11–13 (2.72–2.19 Mb about half of the genes are more similar to genes of fungal species other than F. graminearum. Interestingly, most of the genes on chromosomes 14–16 (1.57–0.56 Mb) are more similar to genes in other fungi than to genes in F. graminearum, suggesting that these chromosomes are either enriched for very ancient sequences lost from F. graminearum, or the genes were horizontally transferred into N. haematococca MPVI from distantly related fungi. In particular, 20.3%, 19.7%, and 41.3% of the genes on chromosomes 14, 15, and 16, respectively, are most similar to sequences from Aspergillus species. Chromosome 14 also corresponds to a previously studied CD chromosome that carries a cluster of genes for pea pathogenicity (PEP genes) [40]. More than 50% of the proteins encoded by genes on chromosome 17 (530 kb) have no significant similarity to genes from any of the eight fungi selected for comparison (Figure 1) suggesting it is also enriched for very ancient sequences or the genes were derived by horizontal transfer.

Figure 1. TBLASTN analysis of genes on each chromosome.

Figure 1

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The relative frequency of the best TBLASTN hits for proteins from each N. haematococca MPVI chromosome. The red line depicts hits to the F. graminearum genome, the yellow line depicts hits to one of the seven other fungal species, and the blue line represents hits to none of the fungal species included in the search.

Presence of orthologs, unique genes, duplicated genes, and possible “pseudoparalogs”

Many gene families in N. haematococca MPVI are larger than the same families in other ascomycetes. In an effort to investigate further the origin of these additional genes, a phylogenetic analysis was carried out on five gene families (ABC transporters, carbohydrate-active enzymes, P450 monooxygenases, binuclear zinc transcription factors, and chromatin genes; Tables S4, S5, S6, S7, S8). This analysis divided the extra genes into two groups: 1) genes specific to N. haematococca MPVI that are not found in F. graminearum and other fungi, and 2) genes that are present as multiple copies in N. haematococca MPVI but are commonly represented by a single copy in other fungi. In some cases the multiple copies (i.e., paralogs) appear to result from lineage-specific gene duplication (Figure 2). However, in other cases the paralogs are more closely related to a gene from distantly related fungal species (often an Aspergillus species); thus, the gene phylogeny does not reflect the species phylogeny (Figure 2). A specific example of this phenomenon is shown in Figure 3 using the phylogenetically conserved ABC transporter gene YOR1. The YOR1 homologs in 27 fungi were identified by a protein similarity search, and their phylogenetic relationship determined (Figure 3). N. haematococca MPVI has two copies of YOR1 (Nh63546 and Nh73313). Nh63546 appears to be an ortholog of YOR1 in F. graminearum (FGSG_07325), which is the closest relative of N. haematococca MPVI included in this analysis. In contrast, Nh73313 does not demonstrate the expected phylogenetic placement (Figure 3). While grouped with other YOR1 homologs, Nh73313 appears distantly related to the F. graminearum YOR1 and its N. haematococca MPVI ortholog, Nh63546. Genes that demonstrate an incongruent phylogenetic topology, as illustrated in Figure 2 and as specifically shown for Nh73313 in Figure 3, have been called ‘pseudoparalogs’ [41]. A pseudoparalog is a copy of a gene that appears paralogous in a single genome analysis, but when sequences from another genome are included, it appears as if the gene were transferred laterally into the genome. However, it has been pointed out recently that the same topology can occur if there is gene duplication, diversification, and differential gene loss (DDL) [42],[43]. Specific and duplicated genes were observed within all five gene families and apparent pseudoparalogous genes were found in all families except the chromatin genes. An example of a pseudoparalog for each gene family is given in the footnotes of Tables S4, S5, S6, S7.

Figure 2. Phylogenetic placement of paralogs in N. haematococca MPVI.

Figure 2

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N. haematococca 1 is the ortholog. (A) Placement of a gene at this position implies a recent gene duplication. (B) Placement of a gene at this position indicates the gene may be a pseudoparalog.

Figure 3. The phylogenetic relationship of the ABC transporter YOR1 from selected fungal genomes.

Figure 3

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Maximum parsimony analysis was used to establish the phylogenetic relationship between the ortholog (Nh63546, red box) and the pseudoparalog (Nh73313, blue box) of N. haematococca MPVI.

Since it has been proposed that HGT could account for some of the genes on the 1.6-Mb CD chromosome of N. haematococca MPVI [34],[36], and four of the five expanded gene families included pseudoparalogs, a global analysis of the genome was undertaken to identify possible pseudoparalogs and genes unique to N. haematococca MPVI. Reciprocal BLASTp searches between the F. graminearum and N. haematococca MPVI proteomes resulted in the identification of 8,922 possible orthologs representing 56.8% of the genes in N. haematococca MPVI. The remaining 6,785 genes in N. haematococca MPVI were identified as ‘unique’ genes. It is within these unique genes that pseudoparalogs are found. To identify possible pseudoparalogs, the unique genes from N. haematococca were compared to the F. graminearum proteome and the orthologs of N. haematococca MPVI with a reciprocal BLASTp approach. A liberal arbitrary cut off of 40% identity over a 40-amino acid length was used to limit the results. A non-stringent cut off for orthologs was used as it created a more comprehensive search for possible pseudoparalogs. Those unique genes that had mutual best hits to both genes of a F. graminearum-N. haematococca MPVI ortholog-pair were classified as possible pseudoparalogs. For example, two CAX (calcium exchange) transporter genes were found in this set; one (Nh65123) is orthologous to F. graminearum FGSG_01606 and the phylogenetic placement of the second, Nh101770, suggests it is a pseudoparalog (Figure S2). Using this approach, 1,331 possible pseudoparalogs were identified (Figure 4). It should be noted that this approach does not differentiate between duplicated and pseudoparalogous genes.

Figure 4. Chromosomal locations of possible pseudoparalogs.

Figure 4

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The percentage for each chromosome is based on the number of possible pseudoparalogs out of the total number of genes on that chromosome.

The G+C percentage and codon usage of orthologs, unique and possible pseudoparalogs in the N. haematococca MPVI genome

Outside of the A+T rich repeated regions typically associated with pericentromeric or centromeric regions, the G+C content is generally consistent among genes within a genome [44],[45]. However, sequences introduced into a genome sometimes retain characteristics of the donor genome. This observation has led to the use of G+C content and codon usage to identify regions in prokaryotic genomes that might have arisen via HGT [44],[46],[47]. The large data set of the groups of genes found in N. haematococca MPVI allowed an analysis of the G+C content of the orthologs to F. graminearum, N. haematococca MPVI unique genes, and possible pseudoparalogs. The overall %G+C content of the orthologs was 55.2% versus 53.3% for the unique genes (P = <2.2×10−16) (Figure 5), while the %G+C of the 3rd position of the codon was 61.5% for the orthologs versus 57.8% for the unique gene set (P = <2.2×10−16) (data not shown). This same overall %G+C difference was observed when the possible pseudoparalogs were compared to the orthologous genes (Figure 5).

Figure 5. G+C content of orthologs, possible pseudoparalogs, and unique genes.

Figure 5

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An analysis of the codon usage of the orthologs and the unique genes was used to identify several differences between the two groups (Table 1). To determine the frequency of each codon for an amino acid, the number of times a particular codon occurred was compared to the occurrence of all the codons for that amino acid. Two of the nine codons that appeared at different frequencies in the two sets of genes, GGG and TTA, had been identified previously as having a different frequency of usage in some of the genes on the CD chromosome compared to genes on other chromosomes [48]. The two codons for the amino acid lysine, in particular, exemplify the difference between the two sets of genes. The codon AAA is used 33,924 times in the set of unique genes but only 30,002 times in the set of orthologous genes, even though the set of orthologous genes is ∼50% larger than the set of unique genes. These codon biases also were observed among the pseudoparalogs, although the smaller number of genes did not allow as many comparisons to be made (data not shown).

Table 1. Differences in codon usage between orthologs and unique genes in N. haematococca MPVI.

Orthologous gene set Unique gene set P-value*
Frequency % Frequency %
G GGG 25856 11.0 30123 16.1 0
GGT 60388 25.7 42247 22.6 0
I ATA 13655 8.3 18167 12.9 6.3×10−250
ATT 52813 32.2 45373 32.2 6.3×10−250
K AAG 138188 82.2 86261 71.8 0
AAA 30002 17.8 33924 28.2 0
L CTC 101513 33.8 72675 28.9 0
TTA 7590 2.5 10569 4.2 0
R CGA 47522 23.0 30574 19.5 0
AGA 25285 12.3 25746 16.4 0
V GTA 15791 7.5 16471 9.8 7.5×10−152
GTC 90688 43.2 68607 40.8 7.5×10−152
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*P-values were determined using Fisher's exact test (2-tailed) to determine the significance of codon usage difference between genes identified as orthologous and those within the unique gene set.

Supernumerary chromosomes

Chromosomes 14–17 are distinctive in their gene content and, as previously mentioned, chromosome 14 is a CD chromosome. In previous studies ([35],[49] and unpublished data) in which isolates were selected for the loss of traits linked to chromosome 14, two isolates (B-33 and HT1) appeared also to have lost another small chromosome. Pulsed field gel electrophoresis experiments revealed that, along with chromosome 14, chromosome 15 appeared to have been lost from B-33 and chromosome 17 appeared to have been lost from HT1 (Figure 6). The loss of all three of these chromosomes was further substantiated by demonstrating that B-33 and HT1 lacked corresponding chromosome-specific sequences on the ends of each assembled chromosome (Figure 7). Therefore, chromosomes 15 and 17, like chromosome 14, are supernumerary chromosomes.

Figure 6. Partial electrophoretic karyotypes of 77-13-7, 77-13-4, and two isolates, B33 and HT-1, derived from 77-13-7 and 77-13-4, respectively.

Figure 6

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Pulsed-Field Gel Electrophoresis conditions that allowed the resolution of the smaller chromosomes were used.

Figure 7. Detection of chromosome-specific sequences found on the ends of chromosomes 14, 15, and 17 in isolates 77-13-7, 77-13-4, and two isolates, B33 and HT-1, derived from 77-13-7 and 77-13-4, respectively.

Figure 7

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Primers from the scaffolds at the ends of the chromosomes were used to produce PCR products from the end of chromosome 14 (A), chromosome 15 (B), and chromosome 17 (C).

Repetitive DNA and RIP

Repeated DNA accounts for 5.1% of the N. haematococca MPVI genome (Table S9). Over half of the repeated sequences (56.1%) are in the unmapped scaffolds, which are 37.2% repetitive. The mapped repeated sequences are unevenly distributed within the genome with chromosomes 14, 15 and 17 containing 32% of the repetitive DNA despite accounting for only 4% of the mapped genome (Table S9, Figure 8). Chromosome 14 is particularly rich in repeats being 21.8% repetitive DNA. Chromosome 14 also contains a disproportionately large number of the DNA transposons found in N. haematococca MPVI (Figure 8).

Figure 8. Distribution of repeat elements in the N. haematococca genome.

Figure 8

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The bar graphs show homologs of previously known or novel transposable elements (Class I, retrotransposons; Class II, DNA transposons; Duplications, repeated regions that are mutated duplicated genes, usually with TE fragments; unknown, repeats that do not match any known or hypothetical proteins). A t-test on the log odds ratio of repetitive and unique fractions of each chromosome revealed that chromosomes 14, 15, and 17 had a higher repetitive content than the other chromosomes (p = 0.01416).

Interestingly, very few of the repeats in N. haematococca MPVI showed a high percentage of identity with each other (Figure S3) suggesting that repeat-induced point mutation (RIP) is potentially involved in the evolution of this genome as it is in N. crassa and other ascomycetes [50],[51]. N. haematococca MPVI has a homolog of RID (RIP defective gene), a putative cytosine methyltransferase that is necessary for RIP [52]. In N. crassa, RIP introduces C∶G to T∶A mutation and the degree of RIP can be assessed by calculating TpA/ApT ratios [51]. When this was done for N. haematococca MPVI, the ratio suggested that 71.6% of the repetitive sequences but only 3.7% of the unique sequences had been subjected to RIP. Specific analysis of select duplicated genes in N. haematococca MPVI also demonstrated the presence of nucleotide changes that are hallmarks of RIP (Figure S4). Finally, RIP was experimentally demonstrated in N. haematococca MPVI by analyzing progeny from a cross in which one parent contained a duplicated gene for hygromycin resistance (hygromycin phosphotransferase, hph). All progeny were hygromycin sensitive, as would be expected if RIP were operative. When a portion of the hph gene from two of the progeny was amplified by PCR, the PCR products had G to A mutations at TpG sites (Figure S5) confirming that RIP is active in N. haematococca MPVI. However, PCR products that represented the entire hph gene and showed no sign of RIP were also obtained from the same progeny. While RIP can occur in N. haematococca MPVI, some additional mechanisms that have been shown to be operative in other fungi, e.g., either ‘methylation induced premeiotically’ (MIP) or the small RNA-dependent ‘quelling’, may be responsible for the silencing of duplicated genes in the absence of point mutations [53],[54]. Indeed, the masc1 gene, a homolog of RID is the sole gene known to be essential for MIP in Ascobolus immersus [55] and all genes known to play essential roles in quelling or meiotic silencing by unpaired DNA (“MSUD”) in N. crassa (52) have homologs in N. haematococca MPVI (data not shown).

Physical properties of genes on specific chromosomes and G+C content of the chromosomes

The small chromosomes of N. haematococca MPVI have several unique properties and these are also observed in the physical properties of their genes (Table S10). The average gene density for the whole genome is 307 genes per Mb (Table S2), but only 223 and 248 genes per Mb for chromosomes 14 and 17, respectively. This may be a reflection of the higher amount of repetitive DNA in these chromosomes. However, the average gene size is also smaller for these chromosomes (1,376 nt for chromosome 14, 1,327 nt for chromosome 15, and 1,484 nt for chromosome 17 versus an average of 1,674 nt for the total genome). The genes on chromosomes 14 and 15 also have fewer exons than the average for the total genome (2.9 versus 3.1) (Tables S2 and S10). In addition, the G+C content (48.2%) of the supernumerary chromosomes is lower than that of the other chromosomes (51.7%) (Table S10).

Location and number of specific genes of interest

Not all gene families in N. haematococca MPVI are exceptionally large. For example, the classes present and number of protein kinases are very similar to S. cerevisiae (Table S11). Because of the diverse habitats and broad host range of N. haematococca MPVI, it might be expected that it would have large numbers of nonribosomal peptide synthetase (NRPS) and polyketide synthetase (PKS) genes as some of these have been shown to synthesize important virulence factors and to contribute to pathogen diversity [56]–[60]. However, the number of NRPS and PKS genes is actually lower than that found in most fungi (Table S12) and these genes are not on the small chromosomes. Another class of genes that has been implicated in the adaptation to ecological niches is that encoding small, secreted proteins [61],[62]. N. haematococca MPVI has 746 of these genes, which is about average for plant pathogens (Table S13).

Discussion

N. haematococca MPVI has a particularly large genome compared to most sequenced ascomycetes. The large number of genes is consistent with its metabolic, ecological, and biological diversity [31],[34]. Among the factors that have contributed to its large size are the supernumeary chromosomes (chromosomes 14, 15, and 17). The mapped portions of these chromosomes contain 418 genes (Table S10). Based on the sizes of these chromosomes as determined by the optical map, 1.5 of 3.5 Mb (approximately 40%) of these chromosomes remains unassigned. One of the unmapped scaffolds contains a gene (MAK1) known to be on a 1.6-Mb CD chromosome in another isolate, 156-30-6 [63]. Thus, there are probably substantially more than 418 genes on these chromosomes.

It has been verified experimentally that at least three N. haematococca MPVI chromosomes (chromosomes 14, 15, and 17) are dispensable (Figures 6 and 7). These supernumerary chromosomes also have relatively few F. graminearum orthologs, but contain unique genes, a disproportionate number of possible pseudoparalogs, a lower G+C content, and a high amount of repetitive DNA with an enrichment of specific types of repeats. Chromosome 14 is a CD chromosome because genes on chromosome 14 increase the habitats available for N. haematococca MPVI [34]. Whether chromosomes 15 and 17 also contribute to the ability of this fungus to occupy more niches and are thereby CD chromosomes, is yet to be established. BLAST searches of the genes on chromosomes 14, 15, and 17 revealed similarity to genes involved in a variety of activities, e.g., biofilm formation, utilization of unique nutrients, etc. (data not shown), which are consistent with the involvement of these genes in habitat specialization. Like chromosome 14, the genes on chromosomes 15 and 17 differ in size from those on the other chromosomes (Table S10).

B chromosomes, a well-known type of supernumerary chromosome [64],[65], also have large amounts of repetitive DNA. However, classical B chromosomes are highly heterochromatic, have very few, if any, active genes, and are for the most part transcriptionally inactive [64]–[66]. In contrast to classical B chromosomes, the CD chromosomes of N. haematococca MPVI contain functional genes for pathogenicity, antibiotic resistance, and the utilization of unique carbon/nitrogen sources [34]. In addition, based on ESTs [67], about 10% of the genes on the small chromosomes are expressed during growth in defined media, even though the BLAST searches did not detect genes involved in essential core functions (data not shown).

The origin of the supernumerary chromosomes is unknown. B chromosomes are often proposed to be derived from A chromosomes (‘normal’ chromosomes) [64]–[66]. However, restriction patterns used to construct the optical map did not reveal regions of similarity between any of N. haematococca MPVI three supernumerary chromosomes and the other chromosomes. Attempts to demonstrate synteny within the N. haematococca MPVI genome by identifying three-gene pairs in the same order and orientation failed to demonstrate any large-scale segmental duplications. However, six paired regions of up to 50 kb were found. Although three of these regions are on chromosome 14, two of the regions are similar to DNA on unmapped scaffolds and one is on chromosome 6, which appears to be part of the main genome complement. The RIP system of N. haematococca MPVI acting on ancient genome duplications in combination with gene loss might conceal any large-scale duplications, if they occurred [68]. It also has been proposed that B chromosomes could arise from A chromosomes following interspecific hybridization in sexual crosses [66]. In this situation, it is possible that the B chromosome would be the only remnants of an original A chromosome. Extending the mechanism of hybridization to include the well-known parasexual cycle that occurs in some fungi provides another explanation of the origin of supernumerary chromosomes in N. haematococca MPVI. As with sexual hybridization there are numerous barriers between vegetative fusion of different fungal species with the major one being vegetative incompatibility [69], which acts at the intraspecies level. However, as recently pointed out by Sanders [70], it would seem unlikely that the barriers to vegetative fusion are so efficient as to prevent entirely the fusion of fungal cells and the subsequent exchange of DNA. Such an event of DNA exchange need not be common; in principle it need happen only once, particularly if the transferred genetic material provided a selective advantage. The exchange of supernumerary chromosomes has been demonstrated in the laboratory, but thus far only between members of the same species [71],[72].

Supernumerary chromosomes do not explain entirely the large size of the N. haematococca MPVI genome. Unique genes on non-supernumerary chromosomes with atypical G+C content and codon preference are also potential pseudoparalogs obtained by HGT [41],[44],[46],[47]. Although HGT has not been considered to be a common mechanism of gene acquisition in fungi [73], there is an increasing number of examples of apparent HGT [74]–[76]. However, the data are often inconclusive and subject to different interpretations [77]. For example, genes may be classified as pseudoparalogs because of lineage-specific gene loss, poor phylogenetic taxon sampling, artifacts due to long branch attraction, and other variables that obfuscate gene origins via DDL or HGT [42],[43]. Many of the properties of the supernumerary chromosomes are similar to genomic islands of A. fumigatus [42]. These species-specific regions of DNA can be as large as 400 kb, and contain A. fumigatus-specific genes and large amounts of repetitive DNA. Genomic islands have smaller genes with fewer exons than the core genes found in Aspergillus species. The genes on these genomic islands, like those on the supernumerary chromosomes, encode metabolic processes that appear to be involved in the adaptation to different ecological niches. The origin of the genes on these islands was originally attributed to HGT, but after comparisons to genomes of additional Aspergillus species, Fedorova et al. [42] proposed that the genes on the genomic islands of Aspergillus arose by DDL. Nevertheless, the ability of N. haematococca MPVI to acquire genes by HGT or a similar mechanism would bypass the evolutionary restraint that an active RIP system places on a fungus by hindering the utility of gene duplications as a means to create new genes functions [50].

The genomic islands of A. fumigatus tend to be located in the sub-telomeric regions of chromosomes. In other fungi, genes involved in specific habitat associations, including pathogenicity, also are often located in the sub-telomeric regions [78]. In N. haematococca MPVI, gene placements differ among the chromosomes. Chromosomes 1, 4, 5, and 9 have most of their unique genes in the sub-telomeric regions (Figure 9). On the other large chromosomes, the unique genes are distributed in clusters throughout the chromosome. Chromosome 7 is unusual in that it is a large chromosome (3.0 Mb) that has some properties of the smaller chromosomes. For example, the majority of the genes on chromosome 7 are unique to N. haematococca MPVI (Figure 9). Chromosome 7 also has the largest number of possible pseudoparalogs (159) of all the chromosomes (Figure 4).

Figure 9. Distribution of orthologs, possible pseudoparalogs, and unique genes on each of the chromosomes of N. haematococca MPVI.

Figure 9

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Compositional statistical analysis using an additive log-ratio transformation [102] reveals that the distribution of genes within the three classes is statistically different on chromosomes 14, 15, and 17 than on the other chromosomes (p = 1.05e-5).

Compositional statistical analysis using an additive log-ratio transformation [102] reveals that the distribution of genes within the three classes is statistically different on chromosomes 14, 15, and 17 than on the other chromosomes (p = 1.05e-5).

Only one isolate of N. haematococca MPVI (77-13-4) was sequenced in the current study and it is a third generation progeny from a laboratory cross between two field isolates. In N. crassa, where RIP was first defined, both RIP and the translocation of chromosomal fragments occur during the sexual cycle [79]. Thus, the crossing of the original isolates of N. haematococca MPVI might have increased the degree of RIP and/or affected the locations of genes in 77-13-4. An examination of field isolates of this fungus has revealed a high degree of chromosomal polymorphism and differences in the locations of studied genes [80]. Thus, it would be of great interest to determine the sequence of field isolates of N. haematococca MPVI and other species within the F. solani species complex and to compare the sizes and organization of their genomes to F. graminearum and to other fusaria in the Gibberella clade.

Materials and Methods

Isolates

N. haematococca MPVI isolates 77-13-4 (FGSC 9596, Fungal Genetics Stock Center) and 77-13-7 are ascospore isolates from a third generation cross between two field isolates: one (T2) obtained from a infected pea plant in NY and the other (T219) obtained from soil in a potato field in PA [81],[82]. Isolates HT1 and B-33 were derived from isolates 77-13-4 and 77-13-7, respectively, after treatment with benomyl and selection for the loss of chromosome 14 [35],[49].

DNA isolation and sequencing

High molecular weight genomic DNA was isolated from protoplasts of 77-13-4 prepared by treatment of mycelia with a combination of lytic enzymes as described previously [83]. Whole-genome shotgun libraries (3-kb, 8-kb, and 40-kb DNA inserts) of 77-13-4 were constructed and sequenced as previously described [84].

RNA isolation and sequencing

Two cDNA libraries were constructed and sequenced to facilitate the automated gene calling programs. The RNA for the cDNA libraries was obtained from mycelia treated in two different ways: both were grown in a rich medium, potato dextrose broth, (PDB) (Difco, Sparks, MD) and one of these was treated with pisatin. Spores of 77-13-4 (105 ml−1) from V8 agar medium [85] were added to 100 ml of PDB in a 250 ml Erlenmeyer flask and grown at room temperature on a rotary shaker (180 rpm) for 24 h. The mycelium was collected by vacuum filtration and stored at −80°C. Mycelium for the pisatin-treated library was collected after overnight growth in PDB, washed in 10 ml of 0.7 M NaCl, and added to 50 ml of phosphate buffer (50 mM potassium phosphate buffer, pH 6.5). After 2 hours, pisatin in DMSO was added to a final concentration of 30 µg of pisatin/ml and 1% DMSO and after another 4.5 hours the mycelium was collected and frozen at −80°C.

For RNA isolation the mycelia were lyophilized and ground under liquid nitrogen using a mortar and pestle. RNA was isolated as described in Mandel et al. [86] with the exception that ground mycelia (200 mg) were placed in an RNase-free 2-ml tube, to which 0.7 ml of ice-cold breaking buffer was added, the mycelia were suspended, and 0.6 ml of acid phenol added. cDNA libraries were constructed and sequenced as described previously with minor differences that include: the size ranges of cDNA (0.6 k–2 kb and >2 kb), the cloning vector (pMCL200cDNA), and the sequencing primers (Fw: 5′-AGGAAACAGCTATGACCA-3′, Rv: 5′-GTTTTCCCAGTCACGACGTTGTA-3′) [87]. 24,793 ESTs were obtained from mycelium grown in the PDB medium and 8,327 from the mycelium treated with pisatin.

Genome finishing methods

Initial read layouts from the whole genome shotgun assembly were converted into a Phred/Phrap/Consed pipeline [88] and, following manual inspection of the assembled sequences, finishing was performed by resequencing plasmid subclones and by walking on plasmid subclones or fosmids using custom primers. All finishing reactions were performed with 4∶1 BigDye to dGTP BigDye terminator chemistry (Applied Biosystems). Repeats in the sequence were resolved by transposon-hopping 8-kb plasmid clones. Fosmid clones were shotgun sequenced and finished to fill large gaps, resolve large repeats, and to extend into chromosome telomere regions where possible. After finishing, the genome remained in 209 scaffolds as a result of many regions of the genome being apparently unclonable in the shotgun libraries constructed for this project.

The resulting assembly was joined and validated by alignments to a N. haematococca optical map (generated by digestion with NheI), with 92.26% of the sequence (72 scaffolds or 47,191,137 bp) being placed onto 17 chromosome optical maps. 3,958,438 bp of the 137 smaller scaffolds remain unplaced because of the lack of sufficient restriction sites. The genome consists of 51,149,575 base pairs of finished sequence with an estimated error rate of less than 1 error in 100,000 bp.

Optical mapping

Protoplasts of 77-13-4, made as described above [83], were directly lysed with a protoplast lysing solution (10 mM EDTA, 5 mM EGTA, 1 mg/ml proteinase K, pH 8.0) by heating to 65°C for 30 min to 1 hr and then incubating overnight at 37°C. The protoplast concentration was adjusted to ∼700 protoplasts per microliter. Optical mapping operations followed previously published techniques [89]; briefly, randomly sheared high molecular weight DNA was loaded onto optical mapping chips for restriction digestion by NheI (New England Biolabs). DNA was stained with YOYO-1 fluorochrome (Invitrogen) and the chips were scanned on an automated fluorescence microscope system for image capture, analysis, and map construction [90]. Resulting single-molecule restriction maps were assembled into genome-wide contigs [91],[92] that served as map scaffolds for sequence joining and validation efforts.

Gene prediction and automated annotation

Gene models (15,707) were predicted and automatically annotated using the Joint Genome Institute (JGI) Annotation Pipeline. Several gene predictors were used on repeat masked assembly: ab initio FGENESH and homology-based FGENESH+ [93] and Genewise [94]. The predicted gene models were verified, corrected, and extended using 33,142 N. haematococca MPVI ESTs. All predicted gene models were functionally annotated by homology to proteins from the NCBI non-redundant set and classified according to Gene Ontology [95], eukaryotic orthologous groups (KOGs [96]), and KEGG metabolic pathways [97]. Of the 15,707 models, 93% were complete models, 25% were supported with EST aligment, 94% with NR alignment, 73% with Swissprot alignment and 52% with Pfam alignments. For every locus the ‘best’representative model was selected based on EST and homology support, to produce a non-redundant representative set, subject to manual curation and the analysis described here.

Best fit analysis of N. haematococca to other fungi

Each predicted protein from the N. haematococca MPVI genome was used as a query in a TBLASTN search of a database consisting of the genomes of A. oryzae, A. nidulans, C. globosum, C. immitis, F. graminearum, M. oryzae, N. crassa, and S. cerevisiae. For each protein query, the genome with the best hit below a threshold of 1e-5 was identified.

Phylogenetic analysis

The predicted amino acid sequences for hypothetical proteins were aligned with ClustalW 1.81 [98]. The resulting alignment files were imported into MacClade 4.08 [99] for manual editing and exclusion of all ambiguously aligned regions. Heuristic searches for maximum parsimony (MP) were conducted in PAUP* [100], and neighbor-joining distance trees were generated in MacVector 10.0.2 (Symantec Corporation). Statistical support was calculated from 1,000 bootstrap replicates.

RIP index

To identify repetitive regions of the genome in the absence of a curated repeat library, the genome was divided into 1 kb non-overlapping windows and BLASTN was used to align each against the complete genome. If the only match greater than 500 bp was a self-match, then the window was labeled as unique, otherwise it was labeled as repetitive. For each 1 kb window, the ratio of TpA/ApT frequency was calculated.

Experimental demonstration of RIP

A transformant (Tr78.2) of N. haematococca that contained several copies of the hph gene from the transformation vector pCWHyg1 [101], was crossed (cross 370) with 77-15-7 as previously described [82]. The hph gene is linked to the homoserine utilization phenotype (HUT) in Tr78.2 and 370 progeny were screened for hygromycin sensitivity and HUT. All forty progeny from cross 370 were sensitive to hygromycin, and half were HUT+. DNA was isolated from two hygromycin sensitive/HUT+ isolates (370-4 and 370-8) as previously described [32]. Sequences of hph were amplified using PCR and the primers Hyg-F (5′-CGGAGATTCGTCGTTCTGAAGAG-3′) and Hyg-R (5′-TTCTACACAGCCATCGGTCCAG-3′) following the manufacturer's protocol (Invitrogen) and the following set of conditions (94°C for 45 sec, 59°C for 30 sec, 72°C for 1.5 min, for 35 cycles). The resulting 1,242 bp products containing hph were cloned into the pGEM T-EZ vector (Promega Corporation, Madison, WI) and hph was sequenced using the Hyg2-F (5′-ACGCGACAACTGAGTGACTG-3′) primer adjacent to hph. Sequencing was done by the Genomic Analysis and Technology Core (GATC) facility at the University of Arizona.

Pulsed-field gel electrophoresis (PFGE) analyses of chromosome-sized DNA

77-13-7 and its derivative B-33 have been described [49], as have 77-13-4 and its derivative HT-1 [35]. The preparation of chromosome-sized DNAs and the PFGE were performed essentially as described previously [35],[72]. For making protoplasts of 77-13-7 and B-33, the enzyme mixture of Garmaroodi and Taga [72] was used, while the enzyme mixture of Rodriguez-Carres et al. [35] was used for 77-13-4 and HT-1. Protoplasts (ca. 3×108 protoplasts/ml) were embedded in low melting temperature agarose (Bio-Rad Laboratories Inc., Hercules, CA) and chromosome-sized DNAs were separated in 0.5× TBE buffer on a 1% (w/v) pulsed field certified agarose (Bio-Rad Laboratories) gel with a contour-clamped homogeneous electric field apparatus (CHEF-DR II, Bio-Rad Laboratories). Running conditions were 5.4 V/cm and pulse time of 120 s for 13 h followed by 180 s for 13 h. Chromosomal DNAs of S. cerevisiae (Bio-Rad Laboratories) were used as the size markers.

Attempts to detect chromosome-specific sequences found on the ends of supernumerary chromosomes

Genomic DNA of N. haematococca MPVI was isolated as previously described [83]. PCR was used to test for the presence of sequences found on the ends of the assembled sequences representing chromosomes 14, 15, and 17 in isolates 77-13-7, 77-13-4, B-33, and HT-1. Primers were designed to amplify regions of scaffolds located on the ends of the respective chromosomes: scaffolds 24 and 62 for chromosome 14, scaffolds 25 and 57 for chromosome 15, and scaffolds 74 and 90 for chromosome 17. These sequences were blasted against the N. haematococca MPVI genome sequence to confirm that they were not found on chromosomes other than those of interest.

Sequences of the primers (Invitrogen) used for PCR were as follows: for chromosome 14, scaffold 24: forward primer, 5′-GCCAGGAGATCGAGATATGA-3′ and reverse primer, 5′-GTGGATGAGATCGGTGTTTC-3′; for chromosome 14, scaffold 62: forward primer, 5′-CTCCATCTTCTCGGCAATGT-3′ and reverse primer, 5′-CTTGGTTCACTCGCATACTTG-3′; for chromosome 15, scaffold 25: forward primer, 5′-GACCGTCAAGGGAGCTACAG-3′ and reverse primer, 5′-ATCAGGGGTCATGTGAAGC-3′; for chromosome 15, scaffold 57: forward primer, 5′-GGCCTTTGTACTCGCATTTA-3′ and reverse primer, 5′-GACCCTCTGCCTTCTTCTTC-3′; for chromosome 17, scaffold 74: forward primer, 5′- CGCCCACTTCTTTGTCTCTA-3′ and reverse primer, 5′-AGCGAATTCATTTGAAGCAG -3′; and for chromosome 17, scaffold 90: forward primer, 5′-GGAGACGTTGATGAGATTGG -3′ and reverse primer, 5′-CATCTGTTGAACCCACACAA -3′. Each reaction had a total volume of 50 µl containing 300 nM forward primer, 300 nM reverse primer, 1 µl (∼50 ng) DNA template, 5 mM MgCl2, 5 µl 10× PCR buffer, 200 µM (each) of dATP, dTTP, dCTP, and dGTP, and 1 unit Taq DNA Polymerase. The PCR protocol consisted of an initial denaturation step of 95°C for 3 min., 35 cycles at 95°C for 30 sec, 55°C for 30 sec, and 72°C for 30 sec, and a final elongation step at 72°C for 30 sec. PCR products were run on a 0.8% agarose gel containing ethidium bromide and visualized under UV light. Genomic DNA of N. haematococca isolates 77-13-4 and 77-13-7 was used as positive controls.

Search for segmental duplications

BlastP searches of the protein set against itself with a threshold value of 1e-20 identified 2,259 gene pairs as best-bidirectional hits. Segmental duplicated regions were defined as genomic regions that share at least three genes in the same order and orientation, while the distance between neighboring gene pairs is less than 50 kb.

Supporting Information

Figure S1

Ratio of the number of genes in gene families in N. haematococca MPVI versus F. graminearum. Only gene families that had ≥10 members in N. haematococca MPVI were used in the analysis. The number of genes per family was derived from Interpro calls made by the JGI for N. haematococca MPVI, and by the Munich Institute for Protein Sequences (MIPS) for F. graminearum.

(1.27 MB TIF)

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Figure S2

The CAX (calcium exchanger) transporter clade from select fungal genomes. Maximum parsimony analysis was used to establish the phylogenetic relationship between the ortholog (Nh65123, red box) and the pseudoparalog (Nh101770, blue box) of N. haematococca MPVI.

(8.06 MB TIF)

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Figure S3

Distibution of repeat identity. NC7 is N. crassa, MG5 is M. oryzae, AN1 is A. nidulans, “FG3 Repeats” is F. graminearum and “FS Repeats” is N. haematococca MPVI.

(4.09 MB TIF)

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Figure S4

Effect of RIP on a family of telomere-associated helicases (TAH) in N. haematococca MPVI. Partial alignment of the 12 predicted TAH genes (tah), spanning only the first three conserved motifs. The top row shows the predicted translation of the fourth tah gene on scaffold 45 (TAH_45-4). While many mutations occur in the wobble position, note the presence of nonsense codons (*). Nucleotides in red (G to A change) and orange (C to T change) can be explained by a single RIP-type mutation, while nucleotides in pink denoted non-RIP-type transversions. Conversion of RIP-type C∶G to T∶A mutations back to the likely original sequence (“de-RIP”, blue), results in a consensus sequence (TAH_ORI) that closely resembles that of the Metarhizium anisopliae TAH1 sequence (MaTAH1; note absence of nonsense codons in the derived consensus sequence, residues in red indicate changes compared to the TAH_45-4 sequence). De-RIP of the complete coding region results in a single large ORF without predicted introns or nonsense codons, similar to the M. anisopliae TAH1 gene (Inglis PW, Rigden DJ, Mello LV, Louis EJ, Valadares-Inglis MC 2005 Monomorphic subtelomeric DNA in the filamentous fungus, Metarhizium anisopliae, contains a RecQ helicase-like gene. Mol Genet Genomics 274: 79–90).

(7.54 MB TIF)

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Figure S5

Repeat-induced point mutation (RIP) in N. haematococca MPVI. The hygromycin resistance (hph) gene is mutated from G to A at multiple TpG positions (indicated in red) in isolates 370-4 and 370-8.

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Table S1

Comparison of genome statistics of several filamentous ascomycete fungi.

(0.06 MB DOC)

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Table S2

Properties of the genes of N. haematococca MPVI.

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Table S3

Gene families that are at least two-fold larger in Nectria haematococca MPVI than in Fusarium graminearum.

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Table S4

The number of ABC transporters in Nectria haematococca MPVI compared to other fungi.

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Table S5

Carbohydrate-active enzymes in N. haematococca MPVI compared to other fungi.

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Table S6

The number of cytochrome P450 genes in Nectria haematococca MPVI compared to other fungi.

(0.06 MB DOC)

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Table S7

Number of predicted genes in Nectria haematococca MPVI that contain transcription factor motifs compared to other fungi.

(0.10 MB DOC)

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Table S8

The number of chromatin genes in N. haematococca MPVI compared to other fungi.

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Table S9

Distribution of repeat elements in the genome of Nectria haematococca MPVI.

(0.08 MB DOC)

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Table S10

Properties of the chromosomes and genes on each chromosome in N. haematococca MPVI.

(0.09 MB DOC)

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Table S11

The protein kinases of N. haematococca MPVI compared to S. cerevisiae.

(0.03 MB DOC)

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Table S12

The number of polyketide synthases (PKS) and nonribosomal peptide synthetases (NRPS) of Nectria haematococca MPVI compared to other fungi.

(0.06 MB DOC)

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Table S13

Distribution of Small Secreted Proteins (SSP) among filamentous Ascomycetes as identified by SignalP.

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Click here for additional data file. (51.5KB, doc)

Footnotes

The authors have declared that no competing interests exist.

This work was performed under the auspices of the US Department of Energy's Office of Science, Biological and Environmental Research Program, and by the University of California, Lawrence Berkeley National Laboratory under Contract DE-AC02-05CH11231, Lawrence Livermore National Laboratory under Contract DE-AC52-07NA27344, and Los Alamos National Laboratory under Contract DE-AC02-06NA25396. Personnel at these laboratories were involved in all aspects of this research. The sequence of Nectria haematococca is available at http://www.jgi.doe.gov/nectria. Partial support for personnel was also obtained from NRA/USDA grant 2008-00645.

References

  • 1.O'Donnell K. Molecular phylogeny of the Nectria haematococca-Fusarium solani species complex. Mycologia. 2000;92:919–938. [Google Scholar]
  • 2.Zhang N, O'Donnell K, Sutton DA, Nalim FA, Summerbell RC, et al. Members of the Fusarium solani species complex that cause infections in both humans and plants are common in the environment. Journal of Clinical Microbiology. 2006;44:2186–2190. doi: 10.1128/JCM.00120-06. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 3.Mandeel QA. Survey of Fusarium species in an arid environment of Bahrain .4. Prevalence of Fusarium species in various soil groups using several isolation techniques. Cryptogamie Mycologie. 1996;17:149–163. [Google Scholar]
  • 4.Farr DF, Bills GF, Chamuris GP, Rossman AY. Fungi on plants and plant products in the United States. St. Paul (Minnesota): American Phytopatholgy Society Press; 1989. 1252 [Google Scholar]
  • 5.Boutati EI, Anaissie EJ. Fusarium, a significant emerging pathogen in patients with hematologic malignancy: Ten years' experience at a cancer center and implications for management. Blood. 1997;90:999–1008. [PubMed] [Google Scholar]
  • 6.Groll AH, Walsh TJ. Uncommon opportunistic fungi: new nosocomial threats. Clinical Microbiology and Infection. 2001;7:8–24. doi: 10.1111/j.1469-0691.2001.tb00005.x. [DOI] [PubMed] [Google Scholar]
  • 7.Guarro J, Gene J. Opportunistic fusarial infections in humans. European Journal of Clinical Microbiology & Infectious Diseases. 1995;14:741–754. doi: 10.1007/BF01690988. [DOI] [PubMed] [Google Scholar]
  • 8.Chang DC, Grant GB, O'Donnell K, Wannemuehler KA, Noble-Wang J, et al. Multistate outbreak of Fusarium keratitis associated with use of a contact lens solution. JAMA-Journal of the American Medical Association. 2006;296:953–963. doi: 10.1001/jama.296.8.953. [DOI] [PubMed] [Google Scholar]
  • 9.Abbas HK, Egley GH. Influence of unrefined corn oil and surface-active agents on the germination and infectivity of Alternaria helianthi. Biocontrol Science and Technology. 1996;6:531–538. [Google Scholar]
  • 10.Chen SY, Dickson DW, Mitchell DJ. Pathogenicity of fungi to eggs of Heterodera glycines. Journal of Nematology. 1996;28:148–158. [PMC free article] [PubMed] [Google Scholar]
  • 11.Bernard EC, Self LH, Tyler DD. Fungal parasitism of soybean cyst nematode, Heterodera glycines (Nemata: Heteroderidae), in differing cropping-tillage regimes. Applied Soil Ecology. 1997;5:57–70. [Google Scholar]
  • 12.Zhdanova NN, Zakharchenko VA, Vember VV, Nakonechnaya LT. Fungi from Chernobyl: mycobiota of the inner regions of the containment structures of the damaged nuclear reactor. Mycological Research. 2000;104:1421–1426. [Google Scholar]
  • 13.Wainwright M, Ali TA, Killham K. Anaerobic growth of fungal mycelium from soil particles onto nutrient-free silica-gel. Mycological Research. 1994;98:761–762. [Google Scholar]
  • 14.Pujol I, Guarro J, Gene J, Sala J. In-vitro antifungal susceptibility of clinical and environmental Fusarium spp. strains. Journal of Antimicrobial Chemotherapy. 1997;39:163–167. doi: 10.1093/jac/39.2.163. [DOI] [PubMed] [Google Scholar]
  • 15.Espinel-Ingroff A. Comparison of in vitro activities of the new triazole SCH56592 and the echinocandins MK-0991 (L-743,872) and LY303366 against opportunistic filamentous and dimorphic fungi and yeasts. Journal of Clinical Microbiology. 1998;36:2950–2956. doi: 10.1128/jcm.36.10.2950-2956.1998. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 16.Dupont J, Jacquet C, Dennetiere B, Lacoste S, Bousta F, et al. Invasion of the French Paleolithic painted cave of Lascaux by members of the Fusarium solani species complex. Mycologia. 2007;99:526–533. doi: 10.3852/mycologia.99.4.526. [DOI] [PubMed] [Google Scholar]
  • 17.Barclay M, Hart A, Knowles CJ, Meeussen JCL, Tett VA. Biodegradation of metal cyanides by mixed and pure cultures of fungi. Enzyme and Microbial Technology. 1998;22:223–231. [Google Scholar]
  • 18.Chakraborty SK, Bhattacharyya A. Degradation of butachlor by 2 soil fungi. Chemosphere. 1991;23:99–105. [Google Scholar]
  • 19.Colombo JC, Cabello M, Arambarri AM. Biodegradation of aliphatic and aromatic hydrocarbons by natural soil microflora and pure cultures of imperfect and lignolitic fungi. Environmental Pollution. 1996;94:355–362. doi: 10.1016/s0269-7491(96)00044-9. [DOI] [PubMed] [Google Scholar]
  • 20.Falcon MA, Rodriguez A, Carnicero A, Regalado V, Perestelo F, et al. Isolation of microorganisms with lignin transformation potential from soil of Tenerife Island. Soil Biology & Biochemistry. 1995;27:121–126. [Google Scholar]
  • 21.Hemida SK, Bagy MMK, Khallil AM. Utilization of hydrocarbons by fungi. Cryptogamie Mycologie. 1993;14:207–213. [Google Scholar]
  • 22.Hsu JC, Camper ND. Degradation of ioxynil by a soil fungus, Fusarium solani. Soil Biology & Biochemistry. 1979;11:19–22. [Google Scholar]
  • 23.Katayama T, Sogo M. An optically-active compound formed by the reduction of an alpha-ketonic lignin substructure model-compound by Fusarium solani m-13-1. Mokuzai Gakkaishi. 1989;35:1116–1124. [Google Scholar]
  • 24.Mitra J, Mukherjee PK, Kale SP, Murthy NBK. Bioremediation of DDT in soil by genetically improved strains of soil fungus Fusarium solani. Biodegradation. 2001;12:235–245. doi: 10.1023/a:1013117406216. [DOI] [PubMed] [Google Scholar]
  • 25.Rafin C, Potin O, Veignie E, Sahraoui ALH, Sancholle M. Degradation of benzo[a]pyrene as sole carbon source by a non white rot fungus, Fusarium solani. Polycyclic Aromatic Compounds. 2000;21:311–329. [Google Scholar]
  • 26.Rodriguez A, Perestelo F, Carnicero A, Regalado V, Perez R, et al. Degradation of natural lignins and lignocellulosic substrates by soil-inhabiting fungi imperfecti. Fems Microbiology Ecology. 1996;21:213–219. [Google Scholar]
  • 27.Romero MC, Salvioli ML, Cazau MC, Arambarri AM. Pyrene degradation by yeasts and filamentous fungi. Environmental Pollution. 2002;117:159–163. doi: 10.1016/s0269-7491(01)00143-9. [DOI] [PubMed] [Google Scholar]
  • 28.Saparrat MCN, Martinez MJ, Tournier HA, Cabello MN, Arambarri AM. Production of ligninolytic enzymes by Fusarium solani strains isolated from different substrata. World Journal of Microbiology & Biotechnology. 2000;16:799–803. [Google Scholar]
  • 29.Walker JRL, Lien BC. Metabolism of fluoroacetate by a soil Pseudomonas sp and Fusarium solani. Soil Biology & Biochemistry. 1981;13:231–235. [Google Scholar]
  • 30.Rossman AY, Samuels GJ, Rogerson CT, Lowen R. Genera of Bionectriaceae, Hypocreaceae and Nectriaceae (Hypocreales, Ascomycetes). Studies in Mycology. 1999;42:1–248. [Google Scholar]
  • 31.VanEtten HD, Kistler HC. Nectria haematococca mating populations I and VI. In: Sidhu GS, editor. Advances in Plant Pathology. New York: Academic Press; 1988. pp. 189–206. [Google Scholar]
  • 32.Miao VP, Covert SF, Vanetten HD. A fungal gene for antibiotic-resistance on a dispensable (B) chromosome. Science. 1991;254:1773–1776. doi: 10.1126/science.1763326. [DOI] [PubMed] [Google Scholar]
  • 33.Covert SF. Supernumerary chromosomes in filamentous fungi. Current Genetics. 1998;33:311–319. doi: 10.1007/s002940050342. [DOI] [PubMed] [Google Scholar]
  • 34.VanEtten HD, Straney D, Covert S, Kistler C. Update on selected topics of the genetics of Nectria haematococca mating population VI with special emphasis on its conditionally dispensable (CD) chromosomes: a source of habitat specific genes. In: Summerell JFL BA, Backhouse D, Bryden WL, Burgess LW, editors. Fusarium. Saint Paul (Minnesota): APS Press; 2001. pp. 97–112. [Google Scholar]
  • 35.Rodriguez-Carres M, White G, Tsuchiya D, Taga M, VanEtten HD. The supernumerary chromosome of Nectria haematococca that carries pea-pathogenicity-related genes also carries a trait for pea rhizosphere competitiveness. Applied and Environmental Microbiology. 2008;74:3849–3856. doi: 10.1128/AEM.00351-08. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 36.Temporini ED, VanEtten HD. An analysis of the phylogenetic distribution of the pea pathogenicity genes of Nectria haematococca MPVI supports the hypothesis of their origin by horizontal transfer and uncovers a potentially new pathogen of garden pea: Neocosmospora boniensis. Current Genetics. 2004;46:29–36. doi: 10.1007/s00294-004-0506-8. [DOI] [PubMed] [Google Scholar]
  • 37.Cuomo CA, Gueldener U, Xu JR, Trail F, Turgeon BG, et al. The Fusarium graminearum genome reveals a link between localized polymorphism and pathogen specialization. Science. 2007;317:1400–1402. doi: 10.1126/science.1143708. [DOI] [PubMed] [Google Scholar]
  • 38.Galagan JE, Henn MR, Ma LJ, Cuomo CA, Birren B. Genomics of the fungal kingdom: Insights into eukaryotic biology. Genome Research. 2005;15:1620–1631. doi: 10.1101/gr.3767105. [DOI] [PubMed] [Google Scholar]
  • 39.Stajich JE, Dietrich FS, Roy SW. Comparative genomic analysis of fungal genomes reveals intron-rich ancestors. Genome Biology. 2007;8:R223. doi: 10.1186/gb-2007-8-10-r223. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 40.Han YN, Liu XG, Benny U, Kistler HC, VanEtten HD. Genes determining pathogenicity to pea are clustered on a supernumerary chromosome in the fungal plant pathogen Nectria haematococca. Plant Journal. 2001;25:305–314. doi: 10.1046/j.1365-313x.2001.00969.x. [DOI] [PubMed] [Google Scholar]
  • 41.Koonin EV. Orthologs, paralogs, and evolutionary genomics. Annual Review of Genetics. 2005;39:309–338. doi: 10.1146/annurev.genet.39.073003.114725. [DOI] [PubMed] [Google Scholar]
  • 42.Fedorova ND, Khaldi N, Joardar VS, Maiti R, Amedeo P, et al. Genomic islands in the pathogenic filamentous fungus Aspergillus fumigatus. Plos Genetics. 2008;4:e1000046. doi: 10.1371/journal.pgen.1000046. doi: 10.1371/journal.pgen.1000046. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 43.Khaldi N, Wolfe KH. Elusive origins of the extra genes in Aspergillus oryzae. PLoS ONE. 2008;3:e3036. doi: 10.1371/journal.pone.0003036. doi: 10.1371/journal.pone.0003036. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 44.Muto A, Osawa S. The guanine and cytosine content of genomic DNA and bacterial evolution. Proc Natl Acad Sci U S A. 1987;84:166–169. doi: 10.1073/pnas.84.1.166. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 45.Karlin S, Campbell AM, Mrazek J. Comparative DNA analysis across diverse genomes. Annual Review of Genetics. 1998;32:185–225. doi: 10.1146/annurev.genet.32.1.185. [DOI] [PubMed] [Google Scholar]
  • 46.Lawrence JG, Ochman H. Molecular archaeology of the Escherichia coli genome. Proc Natl Acad Sci U S A. 1998;95:9413–9417. doi: 10.1073/pnas.95.16.9413. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 47.Ochman H, Lawrence JG, Groisman EA. Lateral gene transfer and the nature of bacterial innovation. Nature. 2000;405:299–304. doi: 10.1038/35012500. [DOI] [PubMed] [Google Scholar]
  • 48.Liu XG, Inlow M, VanEtten HD. Expression profiles of pea pathogenicity (PEP) genes in vivo and in vitro, characterization of the flanking regions of the PEP cluster and evidence that the PEP cluster region resulted from horizontal gene transfer in the fungal pathogen Nectria haematococca. Current Genetics. 2003;44:95–103. doi: 10.1007/s00294-003-0428-x. [DOI] [PubMed] [Google Scholar]
  • 49.VanEtten H, Jorgensen S, Enkerli J, Covert SF. Inducing the loss of conditionally dispensable chromosomes in Nectria haematococca during vegetative growth. Current Genetics. 1998;33:299–303. doi: 10.1007/s002940050340. [DOI] [PubMed] [Google Scholar]
  • 50.Galagan JE, Selker EU. RIP: the evolutionary cost of genome defense. Trends in Genetics. 2004;20:417–423. doi: 10.1016/j.tig.2004.07.007. [DOI] [PubMed] [Google Scholar]
  • 51.Galagan JE, Calvo SE, Borkovich KA, Selker EU, Read ND, et al. The genome sequence of the filamentous fungus Neurospora crassa. Nature. 2003;422:859–868. doi: 10.1038/nature01554. [DOI] [PubMed] [Google Scholar]
  • 52.Freitag M, Williams RL, Kothe GO, Selker EU. A cytosine methyltransferase homologue is essential for repeat-induced point mutation in Neurospora crassa. Proc Natl Acad Sci U S A. 2002;99:8802–8807. doi: 10.1073/pnas.132212899. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 53.Rossignol JL, Faugeron G. MIP - An epigenetic gene silencing process in Ascobolus immersus. 1995. pp. 179–191. Gene Silencing in Higher Plants and Related Phenomena in Other Eukaryotes. [DOI] [PubMed]
  • 54.Catalanotto C, Nolan T, Cogoni C. Homology effects in Neurospora crassa. Fems Microbiology Letters. 2006;254:182–189. doi: 10.1111/j.1574-6968.2005.00037.x. [DOI] [PubMed] [Google Scholar]
  • 55.Malagnac F, Wendel B, Goyon C, Faugeron G, Zickler D, et al. A gene essential for de novo methylation and development in ascobolus reveals a novel type of eukaryotic DNA methyltransferase structure. Cell. 1997;91:281–290. doi: 10.1016/s0092-8674(00)80410-9. [DOI] [PubMed] [Google Scholar]
  • 56.Donadio S, Monciardini P, Sosio M. Polyketide synthases and nonribosomal peptide synthetases: the emerging view from bacterial genomics. Natural Product Reports. 2007;24:1073–1109. doi: 10.1039/b514050c. [DOI] [PubMed] [Google Scholar]
  • 57.Jegorov A, Haiduch M, Sulc M, Havlicek V. Nonribosomal cyclic peptides: specific markers of fungal infections. Journal of Mass Spectrometry. 2006;41:563–576. doi: 10.1002/jms.1042. [DOI] [PubMed] [Google Scholar]
  • 58.Johnson R, Voisey C, Johnson L, Pratt J, Fleetwood D, et al. Distribution of NRPS gene families within the Neotyphodium/Epichloe complex. Fungal Genetics and Biology. 2007;44:1180–1190. doi: 10.1016/j.fgb.2007.04.009. [DOI] [PubMed] [Google Scholar]
  • 59.Lu SW, Kroken S, Lee BN, Robbertse B, Churchill ACL, et al. A novel class of gene controlling virulence in plant pathogenic ascomycete fungi. Proc Natl Acad Sci U S A. 2003;100:5980–5985. doi: 10.1073/pnas.0931375100. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 60.Xu YQ, Orozco R, Wijeratne EMK, Gunatilaka AAL, Stock SP, et al. Biosynthesis of the Cyclooligomer Depsipeptide Beauvericin, a Virulence Factor of the Entomopathogenic Fungus Beauveria bassiana. Chemistry & Biology. 2008;15:898–907. doi: 10.1016/j.chembiol.2008.07.011. [DOI] [PubMed] [Google Scholar]
  • 61.Rep M. Small proteins of plant-pathogenic fungi secreted during host colonization. Fems Microbiology Letters. 2005;253:19–27. doi: 10.1016/j.femsle.2005.09.014. [DOI] [PubMed] [Google Scholar]
  • 62.van der Does HC, Rep M. Virulence genes and the evolution of host specificity in plant-pathogenic fungi. Molecular Plant-Microbe Interactions. 2007;20:1175–1182. doi: 10.1094/MPMI-20-10-1175. [DOI] [PubMed] [Google Scholar]
  • 63.Covert SF, Enkerli J, Miao VPW, VanEtten HD. A gene for maackiain detoxification from a dispensable chromosome of Nectria haematococca. Molecular and General Genetics. 1996;251:397–406. doi: 10.1007/BF02172367. [DOI] [PubMed] [Google Scholar]
  • 64.Camacho JPM, Sharbel TF, Beukeboom LW. B-chromosome evolution. Phil Trans Roy Soc Lond B-Biological Sciences. 2000;355:163–178. doi: 10.1098/rstb.2000.0556. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 65.Jones RN, Gonzalez-Sanchez M, Gonzalez-Garcia M, Vega JM, Puertas MJ. Chromosomes with a life of their own. Cytogenetic and Genome Research. 2008;120:265–280. doi: 10.1159/000121076. [DOI] [PubMed] [Google Scholar]
  • 66.Jones N, Houben A. B chromosomes in plants: escapees from the A chromosome genome? Trends in Plant Science. 2003;8:417–423. doi: 10.1016/S1360-1385(03)00187-0. [DOI] [PubMed] [Google Scholar]
  • 67.Joint Genome Institute. N. haematococca v2.0. 2008. http://genome.jgi-psf.org/Necha2/Necha2.home.html.
  • 68.Semon M, Wolfe KH. Consequences of genome duplication. Current Opinion in Genetics & Development. 2007;17:505–512. doi: 10.1016/j.gde.2007.09.007. [DOI] [PubMed] [Google Scholar]
  • 69.Glass NL, Kaneko I. Fatal attraction: Nonself recognition and heterokaryon incompatibility in filamentous fungi. Eukaryotic Cell. 2003;2:1–8. doi: 10.1128/EC.2.1.1-8.2003. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 70.Sanders IR. Rapid disease emergence through horizontal gene transfer between eukaryotes. Trends in Ecology & Evolution. 2006;21:656–658. doi: 10.1016/j.tree.2006.10.006. [DOI] [PubMed] [Google Scholar]
  • 71.He CZ, Rusu AG, Poplawski AM, Irwin JAG, Manners JM. Transfer of a supernumerary chromosome between vegetatively incompatible biotypes of the fungus Colletotrichum gloeosporioides. Genetics. 1998;150:1459–1466. doi: 10.1093/genetics/150.4.1459. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 72.Garmaroodi HS, Taga M. Duplication of a conditionally dispensable chromosome carrying pea pathogenicity (PEP) gene clusters in Nectria haematococca. Molecular Plant-Microbe Interactions. 2007;20:1495–1504. doi: 10.1094/MPMI-20-12-1495. [DOI] [PubMed] [Google Scholar]
  • 73.Andersson JO. Lateral gene transfer in eukaryotes. Cellular and Molecular Life Sciences. 2005;62:1182–1197. doi: 10.1007/s00018-005-4539-z. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 74.Khaldi N, Collemare J, Lebrun MH, Wolfe KH. Evidence for horizontal transfer of a secondary metabolite gene cluster between fungi. Genome Biology. 2008;9:R18. doi: 10.1186/gb-2008-9-1-r18. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 75.Friesen TL, Stukenbrock EH, Liu ZH, Meinhardt S, Ling H, et al. Emergence of a new disease as a result of interspecific virulence gene transfer. Nature Genetics. 2006;38:953–956. doi: 10.1038/ng1839. [DOI] [PubMed] [Google Scholar]
  • 76.Garcia-Vallve S, Romeu A, Palau J. Horizontal gene transfer of glycosyl hydrolases of the rumen fungi. Molecular Biology and Evolution. 2000;17:352–361. doi: 10.1093/oxfordjournals.molbev.a026315. [DOI] [PubMed] [Google Scholar]
  • 77.Rosewich UL, Kistler HC. Role of horizontal gene transfer in the evolution of fungi. Annual Review of Phytopathology. 2000;38:325–363. doi: 10.1146/annurev.phyto.38.1.325. [DOI] [PubMed] [Google Scholar]
  • 78.Farman ML. Telomeres in the rice blast fungus Magnaporthe oryzae: the world of the end as we know it. Fems Microbiology Letters. 2007;273:125–132. doi: 10.1111/j.1574-6968.2007.00812.x. [DOI] [PubMed] [Google Scholar]
  • 79.Selker EU. Premeiotic instability of repeated sequences in Neurospora crassa. Annual Review of Genetics. 1990;24:579–613. doi: 10.1146/annurev.ge.24.120190.003051. [DOI] [PubMed] [Google Scholar]
  • 80.Miao VPW, Matthews DE, Vanetten HD. Identification and chromosomal locations of a family of cytochrome-P-450 genes for pisatin detoxification in the fungus Nectria haematococca. Molecular & General Genetics. 1991;226:214–223. doi: 10.1007/BF00273606. [DOI] [PubMed] [Google Scholar]
  • 81.Kistler HC, VanEtten HD. 3 non-allelic genes for pisatin demethylation in the fungus Nectria haematococca. Journal of General Microbiology. 1984;130:2595–2603. [Google Scholar]
  • 82.Kistler HC, VanEtten HD. Regulation of pisatin demethylation in Nectria haematococca and its influence on pisatin tolerance and virulence. Journal of General Microbiology. 1984;130:2605–2613. [Google Scholar]
  • 83.Temporini ED, VanEtten HD. Distribution of the pea pathogenicity (PEP) genes in the fungus Nectria haematococca mating population VI. Current Genetics. 2002;41:107–114. doi: 10.1007/s00294-002-0279-x. [DOI] [PubMed] [Google Scholar]
  • 84.Chain P, Lamerdin J, Larimer F, Regala W, Lao V, et al. Complete genome sequence of the ammonia-oxidizing bacterium and obligate chemolithoautotroph Nitrosomonas europaea. Journal of Bacteriology. 2003;185:2759–2773. doi: 10.1128/JB.185.9.2759-2773.2003. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 85.Stevens RB. Mycology Guidebook. Seattle: University of Washington Press; 1974. [Google Scholar]
  • 86.Mandel MA, Galgiani JN, Kroken S, Orbach MJ. Coccidioides posadasii contains single chitin synthase genes corresponding to classes I to VII. Fungal Genetics and Biology. 2006;43:775–788. doi: 10.1016/j.fgb.2006.05.005. [DOI] [PubMed] [Google Scholar]
  • 87.Jeffries TW, Grigoriev IV, Grimwood J, Laplaza JM, Aerts A, et al. Genome sequence of the lignocellulose-bioconverting and xylose-fermenting yeast Pichia stipitis. Nature Biotechnology. 2007;25:319–326. doi: 10.1038/nbt1290. [DOI] [PubMed] [Google Scholar]
  • 88.Gordon D, Abajian C, Green P. Consed: A graphical tool for sequence finishing. Genome Research. 1998;8:195–202. doi: 10.1101/gr.8.3.195. [DOI] [PubMed] [Google Scholar]
  • 89.Zhou S, Bechner MC, Place M, Churas CP, Pape L, et al. Validation of rice genome sequence by optical mapping. BMC Genomics. 2007;8:278. doi: 10.1186/1471-2164-8-278. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 90.Dimalanta ET, Lim A, Runnheim R, Lamers C, Churas C, et al. A microfluidic system for large DNA molecule arrays. Analytical Chemistry. 2004;76:5293–5301. doi: 10.1021/ac0496401. [DOI] [PubMed] [Google Scholar]
  • 91.Valouev A, Schwartz DC, Zhou SG, Waterman MS. An algorithm for assembly of ordered restriction maps from single DNA molecules. Proc Natl Acad Sci U S A. 2006;103:15770–15775. doi: 10.1073/pnas.0604040103. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 92.Zhou S, Herschleb J, Schwartz DC. A Single Molecule System for Whole Genome Analysis. In: Mitchelson KR , editor. New Methods for DNA Sequencing. Amsterdam: Elsevier; 2007. [Google Scholar]
  • 93.Salamov AA, Solovyev VV. Ab initio gene finding in Drosophila genomic DNA. Genome Research. 2000;10:516–522. doi: 10.1101/gr.10.4.516. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 94.Birney E, Durbin R. Using GeneWise in the Drosophila annotation experiment. Genome Research. 2000;10:547–548. doi: 10.1101/gr.10.4.547. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 95.Ashburner M, Ball CA, Blake JA, Botstein D, Butler H, et al. Gene Ontology: tool for the unification of biology. Nature Genetics. 2000;25:25–29. doi: 10.1038/75556. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 96.Koonin EV, Fedorova ND, Jackson JD, Jacobs AR, Krylov DM, et al. A comprehensive evolutionary classification of proteins encoded in complete eukaryotic genomes. Genome Biology. 2004;5:R7. doi: 10.1186/gb-2004-5-2-r7. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 97.Kanehisa M, Goto S, Kawashima S, Okuno Y, Hattori M. The KEGG resource for deciphering the genome. Nucleic Acids Research. 2004;32:D277–D280. doi: 10.1093/nar/gkh063. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 98.Thompson JD, Higgins DG, Gibson TJ. CLUSTAL-W - Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Research. 1994;22:4673–4680. doi: 10.1093/nar/22.22.4673. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 99.Maddison WP, Maddison DR. MacClade. Analysis of phylogeny and character evolution. 3.07 v. Sunderland (Massachusetts): Sinauer Associates; 1997. [Google Scholar]
  • 100.Swofford DL PAUP*. Phylogenetic analysis using parsimony (*and other methods). 4th ed. Sunderland (Massachusetts): Sinauer Associates, Inc; [Google Scholar]
  • 101.Wasmann CC, VanEtten HD. Transformation-mediated chromosome loss and disruption of a gene for pisatin demethylase decrease the virulence of Nectria haematococca on pea. Molecular Plant-Microbe Interactions. 1996;9:793–803. [Google Scholar]
  • 102.Aitchison J. Statistical Analysis of Compositional Data. New York: Chapman and Hall; 1986. [Google Scholar]

Associated Data

This section collects any data citations, data availability statements, or supplementary materials included in this article.

Supplementary Materials

Figure S1

Ratio of the number of genes in gene families in N. haematococca MPVI versus F. graminearum. Only gene families that had ≥10 members in N. haematococca MPVI were used in the analysis. The number of genes per family was derived from Interpro calls made by the JGI for N. haematococca MPVI, and by the Munich Institute for Protein Sequences (MIPS) for F. graminearum.

(1.27 MB TIF)

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Figure S2

The CAX (calcium exchanger) transporter clade from select fungal genomes. Maximum parsimony analysis was used to establish the phylogenetic relationship between the ortholog (Nh65123, red box) and the pseudoparalog (Nh101770, blue box) of N. haematococca MPVI.

(8.06 MB TIF)

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Figure S3

Distibution of repeat identity. NC7 is N. crassa, MG5 is M. oryzae, AN1 is A. nidulans, “FG3 Repeats” is F. graminearum and “FS Repeats” is N. haematococca MPVI.

(4.09 MB TIF)

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Figure S4

Effect of RIP on a family of telomere-associated helicases (TAH) in N. haematococca MPVI. Partial alignment of the 12 predicted TAH genes (tah), spanning only the first three conserved motifs. The top row shows the predicted translation of the fourth tah gene on scaffold 45 (TAH_45-4). While many mutations occur in the wobble position, note the presence of nonsense codons (*). Nucleotides in red (G to A change) and orange (C to T change) can be explained by a single RIP-type mutation, while nucleotides in pink denoted non-RIP-type transversions. Conversion of RIP-type C∶G to T∶A mutations back to the likely original sequence (“de-RIP”, blue), results in a consensus sequence (TAH_ORI) that closely resembles that of the Metarhizium anisopliae TAH1 sequence (MaTAH1; note absence of nonsense codons in the derived consensus sequence, residues in red indicate changes compared to the TAH_45-4 sequence). De-RIP of the complete coding region results in a single large ORF without predicted introns or nonsense codons, similar to the M. anisopliae TAH1 gene (Inglis PW, Rigden DJ, Mello LV, Louis EJ, Valadares-Inglis MC 2005 Monomorphic subtelomeric DNA in the filamentous fungus, Metarhizium anisopliae, contains a RecQ helicase-like gene. Mol Genet Genomics 274: 79–90).

(7.54 MB TIF)

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Figure S5

Repeat-induced point mutation (RIP) in N. haematococca MPVI. The hygromycin resistance (hph) gene is mutated from G to A at multiple TpG positions (indicated in red) in isolates 370-4 and 370-8.

(1.36 MB TIF)

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Table S1

Comparison of genome statistics of several filamentous ascomycete fungi.

(0.06 MB DOC)

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Table S2

Properties of the genes of N. haematococca MPVI.

(0.03 MB DOC)

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Table S3

Gene families that are at least two-fold larger in Nectria haematococca MPVI than in Fusarium graminearum.

(0.09 MB DOC)

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Table S4

The number of ABC transporters in Nectria haematococca MPVI compared to other fungi.

(0.05 MB DOC)

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Table S5

Carbohydrate-active enzymes in N. haematococca MPVI compared to other fungi.

(0.07 MB DOC)

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Table S6

The number of cytochrome P450 genes in Nectria haematococca MPVI compared to other fungi.

(0.06 MB DOC)

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Table S7

Number of predicted genes in Nectria haematococca MPVI that contain transcription factor motifs compared to other fungi.

(0.10 MB DOC)

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Table S8

The number of chromatin genes in N. haematococca MPVI compared to other fungi.

(0.05 MB DOC)

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Table S9

Distribution of repeat elements in the genome of Nectria haematococca MPVI.

(0.08 MB DOC)

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Table S10

Properties of the chromosomes and genes on each chromosome in N. haematococca MPVI.

(0.09 MB DOC)

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Table S11

The protein kinases of N. haematococca MPVI compared to S. cerevisiae.

(0.03 MB DOC)

Click here for additional data file. (30.5KB, doc)
Table S12

The number of polyketide synthases (PKS) and nonribosomal peptide synthetases (NRPS) of Nectria haematococca MPVI compared to other fungi.

(0.06 MB DOC)

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Table S13

Distribution of Small Secreted Proteins (SSP) among filamentous Ascomycetes as identified by SignalP.

(0.05 MB DOC)

Click here for additional data file. (51.5KB, doc)

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