Abstract
Four agar media, Jones-Kendrick (JK) charcoal agar unsupplemented, JK agar supplemented with 0.5 U of penicillin per ml, JK medium supplemented with 2.5 micrograms of methicillin per ml, and JK medium supplemented with 40 micrograms of cephalexin per ml, were evaluated to determine their ability to support growth of Bordetella pertussis, their ability to selectively inhibit normal pharyngeal flora while maintaining growth of B. pertussis, and their stability during storage. Five stock cultures of B. pertussis were plated on each of the media. Penicillin- and cephalexin-supplemented media were more inhibitory for early growth of B. pertussis than was medium supplemented with methicillin. However, after 5 days of incubation at 35 degrees C, all media supported good growth of this organism. When employed to detect B. pertussis in sham specimens, prepared by mixing normal pharyngeal material with each of the five B. pertussis stock cultures, the medium containing cephalexin was judged superior to all other media tested in its combined ability to suppress the growth of normal pharyngeal flora and to allow early detection of Bordetella colonies. All media tested retained their efficacy after 9 weeks of storage at 2 to 8 degrees C.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Cohen S. M., Wheeler M. W. Pertussis Vaccine Prepared with Phase-I Cultures Grown in Fluid Medium. Am J Public Health Nations Health. 1946 Apr;36(4):371–376. [PMC free article] [PubMed] [Google Scholar]
- Jones G. L., Kendrick P. L. Study of a blood-free medium for transport and growth of Bordetella pertussis. Health Lab Sci. 1969 Jan;6(1):40–45. [PubMed] [Google Scholar]
- LACEY B. W. A new selective medium for Haemophilus pertussis, containing a diamidine, sodium fluoride and penicillin. J Hyg (Lond) 1954 Sep;52(3):273–303. doi: 10.1017/s0022172400027492. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MISHULOW L., SHARPE L. S., COHEN L. L. Beef-heart charcoal agar for the preparation of pertussis vaccines. Am J Public Health Nations Health. 1953 Nov;43(11):1466–1472. doi: 10.2105/ajph.43.11.1466. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Regan J., Lowe F. Enrichment medium for the isolation of Bordetella. J Clin Microbiol. 1977 Sep;6(3):303–309. doi: 10.1128/jcm.6.3.303-309.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sutcliffe E. M., Abbott J. D. Selective medium for the isolation of bordetella pertussis and parapertussis. J Clin Pathol. 1972 Aug;25(8):732–733. doi: 10.1136/jcp.25.8.732. [DOI] [PMC free article] [PubMed] [Google Scholar]