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Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1983 Jan;17(1):128–138. doi: 10.1128/jcm.17.1.128-138.1983

Virulence and phenotypic characterization of Yersinia enterocolitica isolated from humans in the United States.

B A Kay, K Wachsmuth, P Gemski, J C Feeley, T J Quan, D J Brenner
PMCID: PMC272587  PMID: 6826697

Abstract

Yersinia enterocolitica was recently reclassified into Yersinia enterocolitica sensu stricto and three additional species. With this new classification, it was of interest to reexamine pathogenicity previously ascribed to Y. enterocolitica. All available clinical isolates of Y. enterocolitica sent to the Centers for Disease Control from 1970 through 1980 were selected for characterization and comparison. One-hundred such strains had been submitted, from 21 states. Most (85%) were biotype 1, and O:8 was the most common of the 24 serotypes encountered. All strains were examined by several virulence assays. Two strains caused conjunctivitis in guinea pigs, 7 were lethal for mice, 54 invaded HEp2 cells, 18 produced a heat-stable enterotoxin, 9 were calcium dependent, 20 autoagglutinated, and 34 had a distinctive colonial morphology at 37 degrees C. Ten isolates of each of the new species that had previously been grouped with Y. enterocolitica (Y. kristensenii, Y. intermedia, and Y. frederiksenii) were characterized and were generally negative in all assays. This study points out pathogenicity differences among Yersinia species, confirms the complex nature of virulence in Y. enterocolitica, and confirms that no single current assay correlates with virulence in Y. enterocolitica.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bauer A. W., Kirby W. M., Sherris J. C., Turck M. Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol. 1966 Apr;45(4):493–496. [PubMed] [Google Scholar]
  2. Bottone E. J. Atypical Yersinia enterocolitica: clinical and epidemiological parameters. J Clin Microbiol. 1978 Jun;7(6):562–567. doi: 10.1128/jcm.7.6.562-567.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bottone E. J., Chester B., Malowany M. S., Allerhand J. Unusual Yersinia enterocolitica isolates not associated with mesenteric lymphadenitis. Appl Microbiol. 1974 May;27(5):858–861. doi: 10.1128/am.27.5.858-861.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Boyce J. M., Evans E. J., Jr, Evans D. G., DuPont H. L. Production of heat-stable, methanol-soluble enterotoxin by Yersinia enterocolitica. Infect Immun. 1979 Aug;25(2):532–537. doi: 10.1128/iai.25.2.532-537.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Dean A. G., Ching Y. C., Williams R. G., Harden L. B. Test for Escherichia coli enterotoxin using infant mice: application in a study of diarrhea in children in Honolulu. J Infect Dis. 1972 Apr;125(4):407–411. doi: 10.1093/infdis/125.4.407. [DOI] [PubMed] [Google Scholar]
  6. Gemski P., Lazere J. R., Casey T. Plasmid associated with pathogenicity and calcium dependency of Yersinia enterocolitica. Infect Immun. 1980 Feb;27(2):682–685. doi: 10.1128/iai.27.2.682-685.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Kapperud G., Berdal B. P., Omland T. Enterotoxin production by Yersinia enterocolitica and Yersinia enterocolitica-like microbes at 22 degrees C and 37 degrees C. Acta Pathol Microbiol Scand B. 1980 Feb;88(1):65–67. doi: 10.1111/j.1699-0463.1980.tb02606.x. [DOI] [PubMed] [Google Scholar]
  8. Keet E. E. Yersinia enterocolitica septicemia. Source of infection and incubation period identified. N Y State J Med. 1974 Nov;74(12):2226–2230. [PubMed] [Google Scholar]
  9. Laird W. J., Cavanaugh D. C. Correlation of autoagglutination and virulence of yersiniae. J Clin Microbiol. 1980 Apr;11(4):430–432. doi: 10.1128/jcm.11.4.430-432.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Pai C. H., Mors V. Production of enterotoxin by Yersinia enterocolitica. Infect Immun. 1978 Mar;19(3):908–911. doi: 10.1128/iai.19.3.908-911.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Pai C. H., Mors V., Toma S. Prevalence of enterotoxigenicity in human and nonhuman isolates of Yersinia enterocolitica. Infect Immun. 1978 Nov;22(2):334–338. doi: 10.1128/iai.22.2.334-338.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Pedersen K. B., Winblad S., Bitsch V. Studies on the interaction between different O-serotypes of Yersinia enterocolitica and HeLa cells. Acta Pathol Microbiol Scand B. 1979 Apr;87B(2):141–145. doi: 10.1111/j.1699-0463.1979.tb02417.x. [DOI] [PubMed] [Google Scholar]
  13. Portnoy D. A., Moseley S. L., Falkow S. Characterization of plasmids and plasmid-associated determinants of Yersinia enterocolitica pathogenesis. Infect Immun. 1981 Feb;31(2):775–782. doi: 10.1128/iai.31.2.775-782.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Quan T. J. Biotypic and serotypic profiles of 367 Yersinia enterocolitica cultures of human and environmental origin in the United States. Contrib Microbiol Immunol. 1979;5:83–87. [PubMed] [Google Scholar]
  15. SERENY B. Experimental shigella keratoconjunctivitis; a preliminary report. Acta Microbiol Acad Sci Hung. 1955;2(3):293–296. [PubMed] [Google Scholar]
  16. Schiemann D. A., Devenish J. A., Toma S. Characteristics of virulence in human isolates of Yersinia enterocolitica. Infect Immun. 1981 Apr;32(1):400–403. doi: 10.1128/iai.32.1.400-403.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Toma S., Lafleur L., Deidrick V. R. Canadian experience with Yersinia enterocolitica (1966--1977). Contrib Microbiol Immunol. 1979;5:144–149. [PubMed] [Google Scholar]
  18. Toma S. Survey on the incidence of Yersinia enterocolitica in the province of Ontario. Can J Public Health. 1973 Sep-Oct;64(5):477–487. [PubMed] [Google Scholar]
  19. Une T. Studies on the pathogenicity of Yersinia enterocolitica. II. Interaction with cultured cells in vitro. Microbiol Immunol. 1977;21(7):365–377. doi: 10.1111/j.1348-0421.1977.tb00301.x. [DOI] [PubMed] [Google Scholar]
  20. Yolken R. H., Greenberg H. B., Merson M. H., Sack R. B., Kapikian A. Z. Enzyme-linked immunosorbent assay for detection of Escherichia coli heat-labile enterotoxin. J Clin Microbiol. 1977 Nov;6(5):439–444. doi: 10.1128/jcm.6.5.439-444.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]

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