Skip to main content
NCBI home page
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1983 Feb;17(2):187–191. doi: 10.1128/jcm.17.2.187-191.1983

Selective recovery of oral Capnocytophaga spp. with sheep blood agar containing bacitracin and polymyxin B.

P A Mashimo, Y Yamamoto, M Nakamura, J Slots
PMCID: PMC272606  PMID: 6833474

Abstract

On the basis of in vitro susceptibility testing of antibiotics, dyes, and other antimicrobial agents, we developed and evaluated a medium, TBBP, for the selective isolation of oral Capnocytophaga spp. TBBP medium consists of 4% Trypticase soy agar (BBL Microbiology Systems, Cockeysville, Md.), 5% sheep blood, 0.1% yeast extract, 50 micrograms of bacitracin per ml, and 100 micrograms of polymyxin B per ml. A total of 34 Capnocytophaga stock cultures grew well on TBBP medium. Except for some streptococcal strains, TBBP medium inhibited growth of all test stock culture isolates of common oral gram-positive and gram-negative species. In a clinical study of 15 deep periodontal pockets, TBBP medium demonstrated Capnocytophaga recoverability that was similar to or higher than that shown by a nonselective blood agar medium. Typical Capnocytophaga colonial morphology enabled us to readily distinguish this organism from the few other bacteria which could grow on TBBP medium.

Full text

PDF
187

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Appelbaum P. C., Ballard J. O., Eyster M. E. Septicemia due to Capnocytophaga (Bacteroides ocharaceus) in Hodgkin's disease. Ann Intern Med. 1979 Apr;90(4):716–717. doi: 10.7326/0003-4819-90-4-716. [DOI] [PubMed] [Google Scholar]
  2. Ellen R. P., Balcerzak-Raczkowski I. B. Differential medium for detecting dental plaque bacteria resembling Actinomyces viscosus and Actinomyces naeslundii. J Clin Microbiol. 1975 Oct;2(4):305–310. doi: 10.1128/jcm.2.4.305-310.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Fitzgerald R. F., Adams B. O. Increased selectivity of Mitis-Salivarius agar containing polymyxin. J Clin Microbiol. 1975 Feb;1(2):239–240. doi: 10.1128/jcm.1.2.239-240.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Forlenza S. W., Newman M. G., Horikoshi A. L., Blachman U. Antimicrobial susceptibility of Capnocytophaga. Antimicrob Agents Chemother. 1981 Jan;19(1):144–146. doi: 10.1128/aac.19.1.144. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Forlenza S. W., Newman M. G., Lipsey A. I., Siegel S. E., Blachman U. Capnocytophaga sepsis: a newly recognised clinical entity in granulocytopenic patients. Lancet. 1980 Mar 15;1(8168 Pt 1):567–568. doi: 10.1016/s0140-6736(80)91057-0. [DOI] [PubMed] [Google Scholar]
  6. Gilligan P. H., McCarthy L. R., Bissett B. K. Capnocytophaga ochracea septicemia. J Clin Microbiol. 1981 Apr;13(4):643–645. doi: 10.1128/jcm.13.4.643-645.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Gold O. G., Jordan H. V., Van Houte J. A selective medium for Streptococcus mutans. Arch Oral Biol. 1973 Nov;18(11):1357–1364. doi: 10.1016/0003-9969(73)90109-x. [DOI] [PubMed] [Google Scholar]
  8. Kilian M., Schiott C. R. Haemophili and related bacteria in the human oral cavity. Arch Oral Biol. 1975 Dec;20(12):791–796. doi: 10.1016/0003-9969(75)90055-2. [DOI] [PubMed] [Google Scholar]
  9. Kornman K. S., Loesche W. J. New medium for isolation of Actinomyces viscosus and Actinomyces naeslundii from dental plaque. J Clin Microbiol. 1978 Jun;7(6):514–518. doi: 10.1128/jcm.7.6.514-518.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Newman M. G., Hulem C., Colgate J., Anselmo C. Antibacterial susceptibility of plaque bacteria. J Dent Res. 1979 Jul;58(7):1722–1732. doi: 10.1177/00220345790580071401. [DOI] [PubMed] [Google Scholar]
  11. Newman M. G., Socransky S. S., Savitt E. D., Propas D. A., Crawford A. Studies of the microbiology of periodontosis. J Periodontol. 1976 Jul;47(7):373–379. doi: 10.1902/jop.1976.47.7.373. [DOI] [PubMed] [Google Scholar]
  12. Newman M. G., Sutter V. L., Pickett M. J., Blachman U., Greenwood J. R., Grinenko V., Citron D. Detection, identification, and comparison of Capnocytophaga, Bacteroides ochraceus, and DF-1. J Clin Microbiol. 1979 Oct;10(4):557–562. doi: 10.1128/jcm.10.4.557-562.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Newman M., Angel I., Karge H., Weiner M., Grinenko V., Schusterman L. Bacterial studies of the Papillon-Lefévre syndrome. J Dent Res. 1977 May;56(5):545–545. doi: 10.1177/00220345770560052201. [DOI] [PubMed] [Google Scholar]
  14. Slots J., Reynolds H. S., Genco R. J. Actinobacillus actinomycetemcomitans in human periodontal disease: a cross-sectional microbiological investigation. Infect Immun. 1980 Sep;29(3):1013–1020. doi: 10.1128/iai.29.3.1013-1020.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Slots J. Selective medium for isolation of Actinobacillus actinomycetemcomitans. J Clin Microbiol. 1982 Apr;15(4):606–609. doi: 10.1128/jcm.15.4.606-609.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Slots J. The predominant cultivable organisms in juvenile periodontitis. Scand J Dent Res. 1976 Jan;84(1):1–10. doi: 10.1111/j.1600-0722.1976.tb00454.x. [DOI] [PubMed] [Google Scholar]
  17. Socransky S. S., Holt S. C., Leadbetter E. R., Tanner A. C., Savitt E., Hammond B. F. Capnocytophaga: new genus of gram-negative gliding bacteria. III. Physiological characterization. Arch Microbiol. 1979 Jul;122(1):29–33. doi: 10.1007/BF00408042. [DOI] [PubMed] [Google Scholar]
  18. Sutter V. L., Pyeatt D., Kwok Y. Y. In vitro susceptibility of Capnocytophaga strains to 18 antimicrobial agents. Antimicrob Agents Chemother. 1981 Aug;20(2):270–271. doi: 10.1128/aac.20.2.270. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Williams B. L., Hollis D., Holdeman L. V. Synonymy of strains of Center for Disease Control group DF-1 with species of Capnocytophaga. J Clin Microbiol. 1979 Oct;10(4):550–556. doi: 10.1128/jcm.10.4.550-556.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES