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. 1983 Feb;17(2):267–271. doi: 10.1128/jcm.17.2.267-271.1983

Characterization of coagulase-negative staphylococci from urinary tract specimens.

L E Nicolle, S A Hoban, G K Harding
PMCID: PMC272620  PMID: 6833480

Abstract

Species of coagulase-negative staphylococci isolated from urine specimens submitted from both inpatients and outpatients to the clinical microbiology laboratory of a teaching hospital were identified with a biotyping system, with species then correlated by clinical features and antimicrobial susceptibility. Of 145 isolates, 102 (70%) were Staphylococcus epidermidis, 24 (17%) were Staphylococcus saprophyticus, 7 (4.7%) were Staphylococcus haemolyticus, 4 (2.8%) were Staphylococcus hominis, 3 (2.1%) were Staphylococcus simulans, and 5 (3.4%) were other species. Features characterizing persons with bacteriuria with S. saprophyticus compared with bacteriuria with any other species included female sex (95% versus 52%), young age (median age, 22 years versus 61 years), ambulatory status (hospital outpatients, 86% versus 23%), and absence of indwelling catheters (4.5% versus 49%). All other coagulase-negative staphylococci were isolated in a setting suggesting nosocomial acquisition, were more frequently resistant to common antimicrobial agents (42% multiply resistant versus 4.2% of S. saprophyticus), and were not distinguished by clinical features. Novobiocin susceptibility, with a sensitivity of 100% and specificity of 96%, provided a simple and reliable test for differentiation of S. saprophyticus from other coagulase-negative staphylococci and should be routinely used for urinary tract specimens in the clinical laboratory.

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Selected References

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  1. Anderson J. D., Clarke A. M., Anderson M. E., Isaac-Renton J. L., McLoughlin M. G. Urinary tract infections due to Staphylococcus saprophyticus biotype 3. Can Med Assoc J. 1981 Feb 15;124(4):415–418. [PMC free article] [PubMed] [Google Scholar]
  2. Facklam R. R., Smith P. B. The gram positive cocci. Hum Pathol. 1976 Mar;7(2):187–194. doi: 10.1016/s0046-8177(76)80022-6. [DOI] [PubMed] [Google Scholar]
  3. Hovelius B., Mårdh P. A., Bygren P. Urinary tract infections caused by Staphylococcus saprophyticus: recurrences and complications. J Urol. 1979 Nov;122(5):645–647. doi: 10.1016/s0022-5347(17)56541-6. [DOI] [PubMed] [Google Scholar]
  4. Hovelius B., Mårdh P. A. On the diagnosis of coagulase-negative staphylococci with emphasis on Staphylococcus saprophyticus. Acta Pathol Microbiol Scand B. 1977 Dec;85B(6):427–434. doi: 10.1111/j.1699-0463.1977.tb01998.x. [DOI] [PubMed] [Google Scholar]
  5. John J. F., Jr, Gramling P. K., O'Dell N. M. Species identification of coagulase-negative staphylococci from urinary tract isolates. J Clin Microbiol. 1978 Oct;8(4):435–437. doi: 10.1128/jcm.8.4.435-437.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Jordan P. A., Iravani A., Richard G. A., Baer H. Urinary tract infection caused by Staphylococcus saprophyticus. J Infect Dis. 1980 Oct;142(4):510–515. doi: 10.1093/infdis/142.4.510. [DOI] [PubMed] [Google Scholar]
  7. Kloos W. E., Schleifer K. H. Simplified scheme for routine identification of human Staphylococcus species. J Clin Microbiol. 1975 Jan;1(1):82–88. doi: 10.1128/jcm.1.1.82-88.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kloos W. E., Wolfshohl J. F. Identification of Staphylococcus species with the API STAPH-IDENT system. J Clin Microbiol. 1982 Sep;16(3):509–516. doi: 10.1128/jcm.16.3.509-516.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Marsik F. J., Brake S. Species identification and susceptibility to 17 antibiotics of coagulase-negative staphylococci isolated from clinical specimens. J Clin Microbiol. 1982 Apr;15(4):640–645. doi: 10.1128/jcm.15.4.640-645.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Meers P. D., Whyte W., Sandys G. Coagulase-negative staphylococci and micrococci in urinary tract infections. J Clin Pathol. 1975 Apr;28(4):270–273. doi: 10.1136/jcp.28.4.270. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Mitchell R. G. Classification of Staphylococcus albus strains isolated from the urinary tract. J Clin Pathol. 1968 Jan;21(1):93–96. doi: 10.1136/jcp.21.1.93. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Namavar F., De Graaff J., De With C., MacLaren D. M. Novobiocin resistance and virulence of strains of Staphylococcus saprophyticus isolated from urine and skin. J Med Microbiol. 1978 Aug;11(3):243–248. doi: 10.1099/00222615-11-3-243. [DOI] [PubMed] [Google Scholar]
  13. Oeding P., Digranes A. Classification of coagulase-negative staphylococci in the diagnostic laboratory. Acta Pathol Microbiol Scand B. 1977 Apr;85(2):136–142. doi: 10.1111/j.1699-0463.1977.tb01687.x. [DOI] [PubMed] [Google Scholar]
  14. Price S. B., Flournoy D. J. Comparison of antimicrobial susceptibility patterns among coagulase-negative staphylococci. Antimicrob Agents Chemother. 1982 Mar;21(3):436–440. doi: 10.1128/aac.21.3.436. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Schleifer K. H., Kloos W. E. A simple test system for the separation of staphylococci from micrococci. J Clin Microbiol. 1975 Mar;1(3):337–338. doi: 10.1128/jcm.1.3.337-338.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Sellin M., Cooke D. I., Gillespie W. A., Sylvester D. G., Anderson J. D. Micrococcal urinary-tract infections in young women. Lancet. 1975 Sep 27;2(7935):570–572. doi: 10.1016/s0140-6736(75)90166-x. [DOI] [PubMed] [Google Scholar]
  17. Shrestha T. L., Darrell J. H. Urinary infection with coagulase-negative staphylococci in a teaching hospital. J Clin Pathol. 1979 Mar;32(3):299–302. doi: 10.1136/jcp.32.3.299. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Wallmark G., Arremark I., Telander B. Staphylococcus saprophyticus: a frequent cause of acute urinary tract infection among female outpatients. J Infect Dis. 1978 Dec;138(6):791–797. doi: 10.1093/infdis/138.6.791. [DOI] [PubMed] [Google Scholar]
  19. Wilkinson B. J., Maxwell S., Schaus S. M. Classification and characteristics of coagulase-negative, methicillin-resistant staphylococci. J Clin Microbiol. 1980 Aug;12(2):161–166. doi: 10.1128/jcm.12.2.161-166.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Williams D. N., Lund M. E., Blazevic D. J. Significance of urinary isolates of coagulase-negative Micrococcaceae. J Clin Microbiol. 1976 Jun;3(6):556–559. doi: 10.1128/jcm.3.6.556-559.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]

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