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Affinity determination by flow cytometry. Increasing concentrations of both IgG1 m971 and m972 were incubated with BJAB cells in a flow cytometry assay as described in the Methods. (A) the histogram of the flow cytometry. (B) the median fluorescence intensities from the flow cytometry were plotted against the corresponding concentrations used for both IgG1s m971 and m972.
