Skip to main content
PMC home page
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1983 Mar;17(3):516–520. doi: 10.1128/jcm.17.3.516-520.1983

Comparison of the Staph-Ident System with a Conventional Method for Species Identification of Urine and Blood Isolates of Coagulase-Negative Staphylococci

Kenneth E Aldridge 1, Charles W Stratton 2, Lyndell S Patterson 2, Martin E Evans 2, Rondy L Hodges 3
PMCID: PMC272677  PMID: 6841586

Abstract

The Staph-Ident system (Analytab Products) for species identification of coagulase-negative staphylococci was compared with the conventional method of Kloos and Schleifer (21). A total of 101 clinical isolates from urine cultures and 95 clinical isolates from blood cultures were studied: overall agreement between the two methods was 86%. We concluded that the Staph-Ident system is a practical test for most clinical microbiology laboratories and that results obtained from this rapid test are comparable to those obtained from the more cumbersome conventional method. Additional investigations are needed to determine the clinical relevance of such species identification.

Full text

PDF
516

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aldridge K. E. Coagulase-negative staphylococci. Infect Control. 1982 Mar-Apr;3(2):161–165. doi: 10.1017/s0195941700055958. [DOI] [PubMed] [Google Scholar]
  2. BRANDT L., SWAHN B. Subacute bacterial endocarditis due to coagulase-negative Staphylococcus albus. Acta Med Scand. 1960 Feb 17;166:125–132. doi: 10.1111/j.0954-6820.1960.tb17362.x. [DOI] [PubMed] [Google Scholar]
  3. Bailey R. R. Significance of coagulase-negative Staphylococcus in urine. J Infect Dis. 1973 Feb;127(2):179–182. doi: 10.1093/infdis/127.2.179. [DOI] [PubMed] [Google Scholar]
  4. Bayston R., Penny S. R. Excessive production of mucoid substance in staphylococcus SIIA: a possible factor in colonisation of Holter shunts. Dev Med Child Neurol Suppl. 1972;27:25–28. doi: 10.1111/j.1469-8749.1972.tb09769.x. [DOI] [PubMed] [Google Scholar]
  5. Bender J. W., Hughes W. T. Fatal Staphylococcus epidermidis sepsis following bone marrow transplantation. Johns Hopkins Med J. 1980 Jan;146(1):13–15. [PubMed] [Google Scholar]
  6. Breckinridge J. C., Bergdoll M. S. Outbreak of food-borne gastroenteritis due to a coagulase-negative enterotoxin-producing staphylococcus. N Engl J Med. 1971 Mar 11;284(10):541–543. doi: 10.1056/NEJM197103112841010. [DOI] [PubMed] [Google Scholar]
  7. CALLAGHAN R. P., COHEN S. J., STEWART G. T. Septicaemia due to colonization of Spitz-Holter valves by staphylococci. Five cases treated with methicillin. Br Med J. 1961 Mar 25;1(5229):860–863. doi: 10.1136/bmj.1.5229.860. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Christensen G. D., Bisno A. L., Parisi J. T., McLaughlin B., Hester M. G., Luther R. W. Nosocomial septicemia due to multiply antibiotic-resistant Staphylococcus epidermidis. Ann Intern Med. 1982 Jan;96(1):1–10. doi: 10.7326/0003-4819-96-1-1. [DOI] [PubMed] [Google Scholar]
  9. Dismukes W. E., Karchmer A. W., Buckley M. J., Austen W. G., Swartz M. N. Prosthetic valve endocarditis. Analysis of 38 cases. Circulation. 1973 Aug;48(2):365–377. doi: 10.1161/01.cir.48.2.365. [DOI] [PubMed] [Google Scholar]
  10. Feigin R. D., Shackelford P. G., Campbell J., Lyles T. O., Schechter M., Lins R. D. Assessment of the role of Staphylococcus epidermidis as a cause of otitis media. Pediatrics. 1973 Oct;52(4):569–576. [PubMed] [Google Scholar]
  11. Freeman R., Hjersing N. Species of coagulase-negative staphylococci isolated from catheter tips from open-heart surgery patients. Thorax. 1980 May;35(5):359–362. doi: 10.1136/thx.35.5.359. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Gemmell C. G., Roberts E. Toxins and enzymes of coagulase negative staphylococci isolated from human infections. J Hyg Epidemiol Microbiol Immunol. 1974;18(3):276–280. [PubMed] [Google Scholar]
  13. Geraci J. E., Hanson K. C., Giuliani E. R. Endocarditis caused by coagulase-negative staphylococci. Mayo Clin Proc. 1968 Jun;43(6):420–434. [PubMed] [Google Scholar]
  14. Hovelius B., Mårdh P. A., Bygren P. Urinary tract infections caused by Staphylococcus saprophyticus: recurrences and complications. J Urol. 1979 Nov;122(5):645–647. doi: 10.1016/s0022-5347(17)56541-6. [DOI] [PubMed] [Google Scholar]
  15. Hovelius B., Thelin I., Mårdh P. A. Staphylococcus saprophyticus in the aetiology of nongonococcal urethritis. Br J Vener Dis. 1979 Oct;55(5):369–374. doi: 10.1136/sti.55.5.369. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Hurley R. Epidemic conjunctivitis in the newborn associated with coagulase negative staphylococci. J Obstet Gynaecol Br Commonw. 1966 Dec;73(6):990–992. doi: 10.1111/j.1471-0528.1966.tb06127.x. [DOI] [PubMed] [Google Scholar]
  17. Jordan P. A., Iravani A., Richard G. A., Baer H. Urinary tract infection caused by Staphylococcus saprophyticus. J Infect Dis. 1980 Oct;142(4):510–515. doi: 10.1093/infdis/142.4.510. [DOI] [PubMed] [Google Scholar]
  18. Keys T. F., Hewitt W. L. Endocarditis due to Micrococci and Staphylococcus epidermidis. Arch Intern Med. 1973 Aug;132(2):216–220. [PubMed] [Google Scholar]
  19. Kloos W. E., Schleifer K. H. Simplified scheme for routine identification of human Staphylococcus species. J Clin Microbiol. 1975 Jan;1(1):82–88. doi: 10.1128/jcm.1.1.82-88.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Liekweg W. G., Jr, Greenfield L. J. Vascular prosthetic infections: collected experience and results of treatment. Surgery. 1977 Mar;81(3):335–342. [PubMed] [Google Scholar]
  21. Mabeck C. E. Significance of coagulase-negative staphylococcal bacteriuria. Lancet. 1969 Nov 29;2(7631):1150–1152. doi: 10.1016/s0140-6736(69)92481-7. [DOI] [PubMed] [Google Scholar]
  22. Maki D. G. Nosocomial bacteremia. An epidemiologic overview. Am J Med. 1981 Mar;70(3):719–732. doi: 10.1016/0002-9343(81)90603-3. [DOI] [PubMed] [Google Scholar]
  23. Marsik F. J., Brake S. Species identification and susceptibility to 17 antibiotics of coagulase-negative staphylococci isolated from clinical specimens. J Clin Microbiol. 1982 Apr;15(4):640–645. doi: 10.1128/jcm.15.4.640-645.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Maskell R. Importance of coagulase-negative staphylococci as pathogens in the urinary tract. Lancet. 1974 Jun 8;1(7867):1155–1158. doi: 10.1016/s0140-6736(74)90634-5. [DOI] [PubMed] [Google Scholar]
  25. Mårdh P. A., Colleen S., Hovelius B. Attachment of bacteria to exfoliated cells from the urogenital tract. Invest Urol. 1979 Mar;16(5):322–326. [PubMed] [Google Scholar]
  26. Patterson F. P., Brown C. S. The McKee-Farrar total hip replacement. Preliminary results and complications of 368 operations performed in five general hospitals. J Bone Joint Surg Am. 1972 Mar;54(2):257–275. [PubMed] [Google Scholar]
  27. Rubin J., Rogers W. A., Taylor H. M., Everett E. D., Prowant B. F., Fruto L. V., Nolph K. D. Peritonitis during continuous ambulatory peritoneal dialysis. Ann Intern Med. 1980 Jan;92(1):7–13. doi: 10.7326/0003-4819-92-1-7. [DOI] [PubMed] [Google Scholar]
  28. Schoenbaum S. C., Gardner P., Shillito J. Infections of cerebrospinal fluid shunts: epidemiology, clinical manifestations, and therapy. J Infect Dis. 1975 May;131(5):543–552. doi: 10.1093/infdis/131.5.543. [DOI] [PubMed] [Google Scholar]
  29. Sewell C. M., Clarridge J. E., Young E. J., Guthrie R. K. Clinical significance of coagulase-negative staphylococci. J Clin Microbiol. 1982 Aug;16(2):236–239. doi: 10.1128/jcm.16.2.236-239.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Sitges-Serra A., Puig P., Jaurrieta E., Garau J., Alastrue A., Sitges-Creus A. Catheter sepsis due to Staphylococcus epidermidis during parenteral nutrition. Surg Gynecol Obstet. 1980 Oct;151(4):481–483. [PubMed] [Google Scholar]
  31. Speller D. C., Mitchell R. G. Coagulase-negative staphylococci causing endocarditis after cardiac surgery. J Clin Pathol. 1973 Jul;26(7):517–522. doi: 10.1136/jcp.26.7.517. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Stratton C. W. Endemic staphylococcal pseudobacteremia. Infect Control. 1981 May-Jun;2(3):251–252. doi: 10.1017/s0195941700055156. [DOI] [PubMed] [Google Scholar]
  33. Valenton M. J., Brubaker R. F., Allen H. F. Staphylococcus epidermidis (albus) endophthalmitis. Report of two cases after cataract extraction. Arch Ophthalmol. 1973 Feb;89(2):94–96. doi: 10.1001/archopht.1973.01000040096004. [DOI] [PubMed] [Google Scholar]
  34. WILSON T. S., STUART R. D. STAPHYLOCOCCUS ALBUS IN WOUND INFECTION AND IN SEPTICEMIA. Can Med Assoc J. 1965 Jul 3;93:8–16. [PMC free article] [PubMed] [Google Scholar]
  35. Wallmark G., Arremark I., Telander B. Staphylococcus saprophyticus: a frequent cause of acute urinary tract infection among female outpatients. J Infect Dis. 1978 Dec;138(6):791–797. doi: 10.1093/infdis/138.6.791. [DOI] [PubMed] [Google Scholar]
  36. Wilson P. D., Jr, Amstutz H. C., Czerniecki A., Salvati E. A., Mendes D. G. Total hip replacement with fixation by acrylic cement. A preliminary study of 100 consecutive McKee-Farrar prosthetic replacements. J Bone Joint Surg Am. 1972 Mar;54(2):207–236. [PubMed] [Google Scholar]
  37. Wilson P. D., Jr, Salvati E. A., Aglietti P., Kutner L. J. The problem of infection in endoprosthetic surgery of the hip joint. Clin Orthop Relat Res. 1973 Oct;(96):213–221. [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES