Abstract
Three commercial reagents for the rapid identification of group D streptococci by slide agglutination were evaluated. These included SeroSTAT (Scott Laboratories, Fiskeville, R.I.), Streptex (Wellcome Laboratories, Research Triangle Park, N.C.), and Phadebact (Pharmacia Diagnostics, Piscataway, N.J.). The methods included direct colony testing, enzyme extraction with pronase, and broth culture. A total of 72 strains of group D streptococci were tested. The SeroSTAT and Streptex reagents with pronase extraction each identified 65 (90%) of the strains. The SeroSTAT reagent was somewhat more specific since it did not cross-react with other streptococci of the viridans group. The Phadebact reagent was nonreactive. We conclude that the latex reagents can be very useful for the quick recognition of group D streptococci in the clinical laboratory.
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Selected References
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- Christensen P., Kahlmeter G., Jonsson S., Kronvall G. New method for the serological grouping of Streptococci with specific antibodies adsorbed to protein A-containing staphylococci. Infect Immun. 1973 Jun;7(6):881–885. doi: 10.1128/iai.7.6.881-885.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Facklam R. R., Cooksey R. C., Wortham E. C. Evaluation of commercial latex agglutination reagents for grouping streptococci. J Clin Microbiol. 1979 Nov;10(5):641–646. doi: 10.1128/jcm.10.5.641-646.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Klein R. S., Recco R. A., Catalano M. T., Edberg S. C., Casey J. I., Steigbigel N. H. Association of Streptococcus bovis with carcinoma of the colon. N Engl J Med. 1977 Oct 13;297(15):800–802. doi: 10.1056/NEJM197710132971503. [DOI] [PubMed] [Google Scholar]
- Levchak M. E., Ellner P. D. Identification of group D streptococci by SeroSTAT. J Clin Microbiol. 1982 Jan;15(1):58–60. doi: 10.1128/jcm.15.1.58-60.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lue Y. A., Howit I. P., Ellner P. D. Rapid grouping of beta-hemolytic streptococci by latex agglutination. J Clin Microbiol. 1978 Sep;8(3):326–328. doi: 10.1128/jcm.8.3.326-328.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Moellering R. C., Jr, Watson B. K., Kunz L. J. Endocarditis due to group D streptococci. Comparison of disease caused by streptococcus bovis with that produced by the enterococci. Am J Med. 1974 Aug;57(2):239–250. doi: 10.1016/0002-9343(74)90448-3. [DOI] [PubMed] [Google Scholar]
- Slifkin M., Pouchet-Melvin G. R. Evaluation of three commercially available test products for serogrouping beta-hemolytic streptococci. J Clin Microbiol. 1980 Mar;11(3):249–255. doi: 10.1128/jcm.11.3.249-255.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Toala P., McDonald A., Wilcox C., Finland M. Susceptibility of group D streptococcus (enterococcus) to 21 antibiotics in vitro, with special reference to species differences. Am J Med Sci. 1969 Dec;258(6):416–430. doi: 10.1097/00000441-196912000-00006. [DOI] [PubMed] [Google Scholar]
- Waitkins S. A., Ratcliffe J. G., Anderson R. D., Roberts C. Grouping of streptococci by Streptex. J Clin Pathol. 1979 Dec;32(12):1234–1236. doi: 10.1136/jcp.32.12.1234. [DOI] [PMC free article] [PubMed] [Google Scholar]