Abstract
In a controlled evaluation of 6,010 blood cultures, the yield of clinically significant microorganisms was greater from a lysis-centrifugation system (Isolator, Du Pont Co.) than from a nonvented vacuum bottle containing tryptic soy broth with sodium polyanetholesulfonate and CO2 and a vented bottle containing biphasic brain heart infusion medium with sodium polyanetholesulfonate. The Isolator significantly increased the frequency of isolation of Staphylococcus aureus and Candida spp. and significantly decreased the time required for the detection of S. aureus, Pseudomonas aeruginosa, and Candida spp.; however, anaerobic bacteria were recovered significantly more frequently from nonvented bottles with tryptic soy broth, and pneumococci were recovered significantly more frequently from both bottle systems. Contamination of cultures was significantly greater with the Isolator system than with either bottle system. Regardless of the number of blood cultures obtained per septic episode, the Isolator detected microbiologically proven bacteremia or fungemia in a significantly greater number of patients and significantly decreased the time required for detection.
Full text
PDFSelected References
These references are in PubMed. This may not be the complete list of references from this article.
- Dorn G. L., Haynes J. R., Burson G. G. Blood culture technique based on centrifugation: developmental phase. J Clin Microbiol. 1976 Mar;3(3):251–257. doi: 10.1128/jcm.3.3.251-257.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dorn G. L., Land G. A., Wilson G. E. Improved blood culture technique based on centrifugation: clinical evaluation. J Clin Microbiol. 1979 Mar;9(3):391–396. doi: 10.1128/jcm.9.3.391-396.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Finegold S. M., White M. L., Ziment I., Winn W. R. Rapid diagnosis of bacteremia. Appl Microbiol. 1969 Sep;18(3):458–463. doi: 10.1128/am.18.3.458-463.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Zierdt C. H., Peterson D. L., Swan J. C., MacLowry J. D. Lysis-filtration blood culture versus conventional blood culture in a bacteremic rabbit model. J Clin Microbiol. 1982 Jan;15(1):74–77. doi: 10.1128/jcm.15.1.74-77.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]