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. 1983 Jun;17(6):958–964. doi: 10.1128/jcm.17.6.958-964.1983

Serological classification of Haemophilus paragallinarum with a hemagglutinin system.

K Kume, A Sawata, T Nakai, M Matsumoto
PMCID: PMC272783  PMID: 6874914

Abstract

Antigens, prepared from 17 strains of Haemophilus paragallinarum by treatment with potassium thiocyanate followed by sonication, uniformly agglutinated glutaraldehyde-fixed chicken erythrocytes and formed specific hemagglutination inhibition antibodies in rabbits. Attempts were made to classify the strains into serotypes by a combination of cross-hemagglutination inhibition and cross-absorption tests, using the hemagglutinating antigens, designated as HA-L hemagglutinin, and their antisera. The cross-hemagglutination inhibition tests showed the existence of three distinct groups among the 17 strains. Further cross-absorption studies indicated that two of the three groups could be subdivided into three serotypes each, forming a total of seven serotypes, designated HA-1 through HA-7. Classification based on the serotype-specific HA-L system was found to be superior in its wider and more clearly defined specificities to other previous classifications, which are based on the agglutination test. There appeared to be a correlation between serotypes and geographic origins of the strains.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bing D. H., Weyand J. G., Stavitsky A. B. Hemagglutination with aldehyde-fixed erythrocytes for assay of antigens and antibodies. Proc Soc Exp Biol Med. 1967 Apr;124(4):1166–1170. doi: 10.3181/00379727-124-31953. [DOI] [PubMed] [Google Scholar]
  2. Gaunt G., Moffat R., Mukkur T. K. Fowl cholera: immunization of chickens with potassium thiocyanate (KSCN) extract of Pasteurella multocida serotype 3. Avian Dis. 1977 Oct-Dec;21(4):543–548. [PubMed] [Google Scholar]
  3. Hinz K. H. Heat-stable antigenic determinants of Haemophilus paragallinarum. Zentralbl Veterinarmed B. 1980;27(8):668–676. doi: 10.1111/j.1439-0450.1980.tb01730.x. [DOI] [PubMed] [Google Scholar]
  4. Iritani Y., Iwaki S., Yamaguchi T., Sueishi T. Determination of types 1 and 2 hemagglutinins in serotypes of Hemophilus paragallinarum. Avian Dis. 1981 Apr-Jun;25(2):479–483. [PubMed] [Google Scholar]
  5. Kume K., Sawata A., Nakase Y. Haemophilus infections in chickens. 3. Immunogenicity of serotypes 1 and 2 strains of Haemophilus paragallinarum. Nihon Juigaku Zasshi. 1980 Dec;42(6):673–680. doi: 10.1292/jvms1939.42.673. [DOI] [PubMed] [Google Scholar]
  6. Kume K., Sawata A., Nakase Y. Haemophilus infections in chickens. I. Characterization of Haemophilus paragallinarum isolated from chickens affected with coryza. Nihon Juigaku Zasshi. 1978 Feb;40(1):65–73. doi: 10.1292/jvms1939.40.65. [DOI] [PubMed] [Google Scholar]
  7. Kume K., Sawata A., Nakase Y. Immunologic relationship between Page's and Sawata's serotype strains of Haemophilus paragallinarum. Am J Vet Res. 1980 May;41(5):757–760. [PubMed] [Google Scholar]
  8. Kume K., Sawata A., Nakase Y. Relationship between protective activity and antigen structure of Haemophilus paragallinarum serotypes 1 and 2. Am J Vet Res. 1980 Jan;41(1):97–100. [PubMed] [Google Scholar]
  9. Matsumoto M., Yamamoto R. Protective quality of an aluminum hydroxide-absorbed broth bacterin against infectious coryza. Am J Vet Res. 1975 Apr;36(4 Pt 2):579–582. [PubMed] [Google Scholar]
  10. PAGE L. A. Haemophilus infections in chickens. I. Characteristics of 12 Haemophilus isolates recovered from diseased chickens. Am J Vet Res. 1962 Jan;23:85–95. [PubMed] [Google Scholar]
  11. Rimler R. B., Davis R. B., Page R. K. Infectious coryza: cross-protection studies, using seven strains of Haemophilus gallinarum. Am J Vet Res. 1977 Oct;38(10):1587–1589. [PubMed] [Google Scholar]
  12. Sawata A., Kume K., Nakase Y. Antigenic structure and relationship between serotypes 1 and 2 of Haemophilus paragallinarum. Am J Vet Res. 1979 Oct;40(10):1450–1453. [PubMed] [Google Scholar]
  13. Sawata A., Kume K., Nakase Y. Biologic and serologic relationships between Page's and Sawata's serotypes of Haemophilus paragallinarum. Am J Vet Res. 1980 Nov;41(11):1901–1904. [PubMed] [Google Scholar]
  14. Sawata A., Kume K., Nakase Y. Haemophilus infections in chickens. 2. Types of Haemophilus paragallinarum Isolates from chickens with infectious coryza, in relation to Haemophilus gallinarum strain no. 221. Nihon Juigaku Zasshi. 1978 Dec;40(6):645–652. doi: 10.1292/jvms1939.40.645. [DOI] [PubMed] [Google Scholar]
  15. Sawata A., Kume K., Nakase Y. Hemagglutinin of Haemophilus paragallinarum serotype 2 organisms: occurrence and immunologic properties of hemagglutinin. Am J Vet Res. 1982 Jul;43(7):1311–1314. [PubMed] [Google Scholar]
  16. Yamaguchi T., Iritani Y. Occurrence of two hemagglutinins on Haemophilus paragallinarum strain 221 and comparison of their properties. Nihon Juigaku Zasshi. 1980 Dec;42(6):709–711. doi: 10.1292/jvms1939.42.709. [DOI] [PubMed] [Google Scholar]

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