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Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1983 Jun;17(6):1061–1065. doi: 10.1128/jcm.17.6.1061-1065.1983

Urine screening with the MS-2.

D J Hoban, J C Koss, C A Gratton, A R Ronald
PMCID: PMC272802  PMID: 6348075

Abstract

A study was undertaken to evaluate the effectiveness of the MS-2 (Abbott Laboratories, Dallas, Tex.) in screening urine specimens in a large clinical laboratory. A total of 15,319 urine specimens (9,954 midstream specimens and 5,365 catheter specimens) were evaluated with the MS-2 and by a surface streak procedure. The study was conducted in two phases, differing in that phase II urine specimens were evaluated in the MS-2 by using a program software update (03.01). For midstream urine specimens, MS-2 detection rates in phases I and II were, respectively, 94.5 and 94.3% at a plate count of greater than 10(5) CFU/ml, 74.4 and 65.3% at plate counts of 5 X 10(4) to 1 X 10(5) CFU/ml, 55.0 and 52.4% at plate counts of 1 X 10(4) to 5 X 10(4) CFU/ml, 31.2 and 20.5% at plate counts of 10(3) to 10(4) CFU/ml, and 15.7 and 6.4% at plate counts of less than 10(3) CFU/ml. For catheter urine specimens, the MS-2 detection rates in phases I and II were, respectively, 95.4 and 96.8% at plate counts greater than 10(5) CFU/ml, 74.4 and 85.7% at plate counts of 5 X 10(4) to 1 X 10(5) CFU/ml, 50.0 and 44.4% at plate counts 10(4) to 5 X 10(4) CFU/ml, 25.6 and 14.9% at plate counts of 10(3) to 10(4) CFU/ml, and 14.7 and 5.2% at plate counts less than 10(3) CFU/ml.

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Selected References

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  1. Alexander D. N., Ederer G. M., Matsen J. M. Evaluation of an adenosine 5'-triphosphate assay as a screening method to detect significant bacteriuria. J Clin Microbiol. 1976 Jan;3(1):42–46. doi: 10.1128/jcm.3.1.42-46.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. BULGER R. J., KIRBY W. M. SIMPLE TESTS FOR SIGNIFICANT BACTERIURIA. Arch Intern Med. 1963 Nov;112:742–746. doi: 10.1001/archinte.1963.03860050129015. [DOI] [PubMed] [Google Scholar]
  3. Barbin G. K., Thorley J. D., Reinarz J. A. Simplified microscopy for rapid detection of significant bacteriuria in random urine specimens. J Clin Microbiol. 1978 Mar;7(3):286–289. doi: 10.1128/jcm.7.3.286-289.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cady P., Dufour S. W., Lawless P., Nunke B., Kraeger S. J. Impedimetric screening for bacteriuria. J Clin Microbiol. 1978 Mar;7(3):273–278. doi: 10.1128/jcm.7.3.273-278.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Jorgensen J. H., Jones P. M. Comparative evaluation of the Limulus assay and the direct Gram stain for detection of significant bacteriuria. Am J Clin Pathol. 1975 Jan;63(1):142–148. doi: 10.1093/ajcp/63.3.142. [DOI] [PubMed] [Google Scholar]
  6. KASS E. H. Asymptomatic infections of the urinary tract. Trans Assoc Am Physicians. 1956;69:56–64. [PubMed] [Google Scholar]
  7. KUNIN C. M. The quantitative significance of bacteria visualized in the unstained urinary sediment. N Engl J Med. 1961 Sep 21;265:589–590. doi: 10.1056/NEJM196109212651207. [DOI] [PubMed] [Google Scholar]
  8. Lewis J. F., Alexander J. Microscopy of stained urine smears to determine the need for quantitative culture. J Clin Microbiol. 1976 Oct;4(4):372–374. doi: 10.1128/jcm.4.4.372-374.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. McCarthy L. R., Gavan T. L., Robson J., Corlett C. Evaluation of the MS-2 urine screening method for detection of bacteriuria. J Clin Microbiol. 1982 Aug;16(2):250–252. doi: 10.1128/jcm.16.2.250-252.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Pezzlo M. T., Tan G. L., Peterson E. M., de la Maza L. M. Screening of urine cultures by three automated systems. J Clin Microbiol. 1982 Mar;15(3):468–474. doi: 10.1128/jcm.15.3.468-474.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. SLEIGH J. D. DETECTION OF BACTERIURIA BY A MODIFICATION OF THE NITRITE TEST. Br Med J. 1965 Mar 20;1(5437):765–767. doi: 10.1136/bmj.1.5437.765. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Stamm W. E., Wagner K. F., Amsel R., Alexander E. R., Turck M., Counts G. W., Holmes K. K. Causes of the acute urethral syndrome in women. N Engl J Med. 1980 Aug 21;303(8):409–415. doi: 10.1056/NEJM198008213030801. [DOI] [PubMed] [Google Scholar]
  13. Wallis C., Melnick J. L., Longoria C. J. Colorimetric method for rapid determination of bacteriuria. J Clin Microbiol. 1981 Sep;14(3):342–346. doi: 10.1128/jcm.14.3.342-346.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]

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