Abstract
Thirty sera from eight patients with disseminated or localized hydatid disease have been examined for the presence of circulating immune complexes (CICs) by the conglutinin-binding assay and for immunoglobulin levels. The highest levels of CICs were of the immunoglobulin A (IgA) class, with lower values of IgG-CIC and IgM-CIC; these results did not correlate, except for IgG, with the free immunoglobulin levels. Efforts to identify parasitic antigen(s) involved in the CIC formation with different methods have been unsuccessful. In the follow-up of each patient, CIC appeared to be better correlated to clinical conditions than to hemagglutination titers. We have concluded that the presence of CIC in hydatid disease is probably an expression of B-cell polyclonal activation and that these complexes are valuable in the clinical monitoring of the disease.
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- BOYDEN S. V. The adsorption of proteins on erythrocytes treated with tannic acid and subsequent hemagglutination by antiprotein sera. J Exp Med. 1951 Feb;93(2):107–120. doi: 10.1084/jem.93.2.107. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bekhti A., Schaaps J. P., Capron M., Dessaint J. P., Santoro F., Capron A. Treatment of hepatic hydatid disease with mebedazole: preliminary results in four cases. Br Med J. 1977 Oct 22;2(6094):1047–1051. doi: 10.1136/bmj.2.6094.1047. [DOI] [PMC free article] [PubMed] [Google Scholar]
- CASTAGNARI L., TOLU A. L'EMOAGGLUTINAZIONE INDIRETTA NELLA DIAGNOSI BIOLOGICA DELL'IDATIDOSI. Policlinico Med. 1964 Dec;71:395–407. [PubMed] [Google Scholar]
- Casali P., Bossus A., Carpentier N. A., Lambert P. H. Solid-phase enzyme immunoassay or radioimmunoassay for the detection of immune complexes based on their recognition by conglutinin: conglutinin-binding test. A comparative study with 125I-labelled Clq binding and Raji-cell RIA tests. Clin Exp Immunol. 1977 Aug;29(2):342–354. [PMC free article] [PubMed] [Google Scholar]
- Cuatrecasas P. Affinity chromatography. Annu Rev Biochem. 1971;40:259–278. doi: 10.1146/annurev.bi.40.070171.001355. [DOI] [PubMed] [Google Scholar]
- D'Amelio R., Brighouse G., Barnet M., Lambert P. H. Antigen-specific detection of soluble immune complexes in conglutinin-binding assays. Clin Exp Immunol. 1981 Aug;45(2):283–289. [PMC free article] [PubMed] [Google Scholar]
- Greenwood B. M. Possible role of a B-cell mitogen in hypergammaglobulinaemia in malaria and trypanosomiasis. Lancet. 1974 Mar 16;1(7855):435–436. doi: 10.1016/s0140-6736(74)92386-1. [DOI] [PubMed] [Google Scholar]
- Maire M. A., Barnet M., Lambert P. H. Purification of bovine conglutinin using pepsin digestion. Mol Immunol. 1981 Feb;18(2):85–89. doi: 10.1016/0161-5890(81)90073-0. [DOI] [PubMed] [Google Scholar]
- McConahey P. J., Dixon F. J. A method of trace iodination of proteins for immunologic studies. Int Arch Allergy Appl Immunol. 1966;29(2):185–189. doi: 10.1159/000229699. [DOI] [PubMed] [Google Scholar]
- NISONOFF A., WISSLER F. C., LIPMAN L. N., WOERNLEY D. L. Separation of univalent fragments from the bivalent rabbit antibody molecule by reduction of disulfide bonds. Arch Biochem Biophys. 1960 Aug;89:230–244. doi: 10.1016/0003-9861(60)90049-7. [DOI] [PubMed] [Google Scholar]
- Sorice F. La diagnosi biologica dell'idatidosi umana. G Mal Infett Parassit. 1966 Mar;18(3):192–228. [PubMed] [Google Scholar]
- Theofilopoulos A. N., Dixon F. J. Immune complexes in human diseases: a review. Am J Pathol. 1980 Aug;100(2):529–594. [PMC free article] [PubMed] [Google Scholar]
- Vergani D., Mieli-Vergani G., Alberti A., Neuberger J., Eddleston A. L., Davis M., Williams R. Antibodies to the surface of halothane-altered rabbit hepatocytes in patients with severe halothane-associated hepatitis. N Engl J Med. 1980 Jul 10;303(2):66–71. doi: 10.1056/NEJM198007103030202. [DOI] [PubMed] [Google Scholar]