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. Author manuscript; available in PMC: 2009 Nov 25.
Published in final edited form as: Biochemistry. 2008 Nov 25;47(47):12540–12550. doi: 10.1021/bi801027k

Fig. 5. Mg(II) Dependence of the Single Turnover Rate Constant kobs.

Fig. 5

Data were collected with 300 nM cognate duplex and 2 uM enzyme dimers at 50 mM Tris, variable MgCl2, and NaCl normalized to constant ionic strength at pH 7.5, 37°C. Data fit to the Hill equation to yield an nH of 4.0 ± 0.3 per homodimer.