Figure 5. Spatial control of adenovirus immobilization to restrict cell transduction at specific sites.

(a) The image of coated PCL surfaces by phase contrast microscope observation. The wax masking technique was utilized to restrict the CVD treatment to only the left of PCL surfaces, on which Amine-PEO₃-Biotin was conjugated to immobilize avidin. (b) Biotin-fluorescein was used to label the surface avidin. Only the exposed area without wax protection expressed green fluorescence. (c) Fibroblasts were cultured on PCL surfaces for 2 days. The transduced cells stained blue after exposure to X-gal staining. Cells grew to confluence on the material surfaces; however, cell transduction was restricted to the non-masked area, which was consistent with the avidin distribution.