Figure 4.—

Effect of the Rx NBLet on Rx function. (A) PVX resistance conferred by Rx in its complete genomic context (Rx:4HA) and without the Rx NBLet (Rx:4HA ΔNBLet). Rx constructs were transiently coexpressed in Nicotiana benthamiana leaves with an infectious PVX:GFP clone via agroinfiltration. PVX:GFP accumulation was monitored as GFP fluorescence under UV light at 5 d after infiltration. PVX:GFP infiltrated in the absence of Rx is shown at bottom left for comparison. (B) Western blot analysis showing Rx accumulation when expressed with and without NBLet. N. benthamiana leaves were infiltrated with Agrobacterium-containing Rx constructs, and samples were collected 2 days later. Rx protein levels were detected by anti-HA immunoblotting.