Fig. 5.

Compromised Ca²⁺ handling in TRIC-B-knockout type II cells. (A) Impaired ATP-evoked Ca²⁺ transients and facilitated Ca²⁺ responses induced by ionomycin (IM) in TRIC-B-knockout type II cells. Fura-2 Ca²⁺ imaging was performed in type II cells derived from more than four neonates of each genotype, and averaged ratiometric traces are shown. (B) Statistical analysis of resting cytoplasmic Ca²⁺ levels in normal and Ca²⁺-free bathing solutions. (C) Statistical analysis of ATP-evoked Ca²⁺ transients and the subsequent ionomycin-induced Ca²⁺ responses. (D) Enhanced ionomycin-induced Ca²⁺ responses and normal store-operated calcium entry (SOCE) activity in TRIC-B-knockout type II cells. (E) Statistical analysis of ionomycin-induced responses and following SOCE activities. The data represent the mean±s.e.m., the numbers of cells examined are shown in parentheses, and statistical differences between the genotypes are marked with asterisks (P<0.01 by Student's t-test).