Figure 5.

Dose-dependent Raji B-cell apoptosis induced by Jacalin. Raji B cells were treated with Jacalin at various concentrations, as indicated above, for 72 hr, followed by assessment of apoptosis. Poly (ADP-ribose) polymerase (PARP) cleavage was analysed by Western blotting for apoptosis detection. The arrows indicate 116 000 PARP (upper bands) and the 89 000 apoptosis-related cleavage fragment (lower bands). As a positive apoptosis control, Raji B cells were exposed to ultraviolet irradiation at 254 nm for 15 min. GAPDH is shown as a control for protein loading (bottom). The results are representative of three independent experiments.